切的

However, CDH has little synergism with cellobiohydrolase or endoglucanase in cellulose degradation. But, if the substrate was pretreated with CDH in the presence of cellobiose, the rate of the hydrolysis of the substrate by cellobiohydrolase or endoglucanase increased.

CDH与纯化的外切纤维素酶或内切纤维素酶或内切纤维素酶之间没有协同作用关系，但在纤维二糖存在条件下底物事先用CDH处理后再用上述纯化的酶水解，作用明显增强。

Cut or used for cutting crosswise or across the grain.

交叉的切、用来交叉的切或者对着横纹切。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

Based on the data of dynamic shear rheometric tests, a constitutive equation was also proposed, which could characterize the dependence of the melt viscosity of PE and its blends on both shear rate and temperature.

此外，在大量mPE和mPE/LDPE共混合金动态剪切流变试验数据的基础上，拟合并推导出能够同时反映PE和mPE/LDPE共混合金熔体粘度对剪切速率和温度依赖性的本构方程，并且通过分析发现，方程中的α值对PE和mPE/LDPE共混合金的熔体粘度的剪切速率依赖性影响较大，α值越大，其熔体粘度对剪切速率越敏感。

Second, on the basis of classical girder theory, girder element stiffness matrix considering shearing deformation is deduced, the method of calculation of value of shearing deformation is illustrated, and how far effect on inner force and deformation considering sheering deformation is discussed. Through comparison inner force and deformation between considerations in shearing deformation and not, several results are achieved and several suggestions to calculation are pointed out.Third, prestressed reinforcement is considered in the paper.

其次，在经典梁理论的基础上，本文推导出考虑剪切变形的平面梁和空间梁单元刚度矩阵，阐述了剪切变形系数的计算方法，讨论了在不同箱梁截面高度情况下，剪切变形对结构内力值和变形值的影响，并与不考虑剪切变形影响的结果作比较，得出有关结论，并对工程设计计算做出了一些建议。

According to this phenomenon, we analyze the original circuit structure of unreasonable, in the original circuit has added a intermediate relay (5J), a limit switch, and take advantage of the original paper cutting, the unused in the buttons on the normally closed contact, specifying the control switch some of cam shape change, so that you solve the Cutter knife to cut the ?

根据这种现象，我们分析了原电路的不合理结构，在原电路中添加了一只中间继电器（5J）、一只行程开关，并利用了原切纸按钮中未用上的常闭触点，同时将控制行程开关的凸轮形状稍加改变，这样就彻底解决了切纸刀切下后自动复位难的问题，使切纸速度达到了要求的次数。

The main products of the sticks is die-cutting on automatic die-cutting machine requires will rely on a pipe of knife more force several narrow-necked, smooth production after the die cutting machine out, in the sense of narrow-necked reflected in the die-cutting on the box of the edge is not smooth.

影响产品美观的主要原因是在平压平自动模切机上进行模切加工时需要将靠叼口边的刀多敲破几个小口，才能顺利地将模切后的成品从机器中拉出来，敲破的小口体现在模切后的盒上是边缘不光滑。

MCP could effectively delay the softening of climacteric fruit, such as Starking and Fuji apples and Feicheng peach, but as different fruits had different softening mechanism, the mechanism of 1-MCP inhibiting different fruit softening was different: in Starking apples, the key enzyme to the softening is endo-and exo-PG, while during Feicheng peach fruit softening, the key enzyme was EGase before the 20th d and endo-PG after 20th d during storage at 0℃. 1-MCP inhibited fruit softening mainly by inhibiting PG activity in Starking apple while in Feicheng peach, by inhibiting the activity of EGase in the earlier storage time and endo-PG in the latter time. It was also found that LOX accelerated the initiation of the softening and was regulated by ethylene and 1-MCP in apple and peach.

2.1-MCP能较好的延缓红星、富士和肥城桃等跃变型果实硬度的下降，但是不同品种/树种的果实软化机理不同，1-MCP抑制其软化的机理也不一致：在红星果实中，对软化起主要作用的酶一直为内切和外切两种PG，而在肥城桃软化过程中，前20d以纤维素酶活性起主要作用，20d后软化以内切PG为主。1-MCP主要通过抑制红星果实中PG酶活性来抑制果实硬度的下降；在肥城桃果实中，1-MCP通过抑制前期纤维素酶和后期内切PG活性，延缓了果实硬度的下降。

The main contents are as follows:Based on the vortex theory and the hydrodynamic basic equations, taking account of the viscosity effect, the expressions of the tangential speed...

主要内容包括：在涡运动理论的基础上，考虑流体的粘性效应，从流体动力学基本方程出发，分析推导了旋流分离器内流体的切向速度和切向应力的分布表达式，定性讨论了旋流分离器外壁的转动对切向速度和切向应力的影响。

"The Second China Paper Packaging Industry corrugated packaging and printing exhibition-cum-high-level strategic seminars, and the second session of the China Paper Packaging Industry Color Printing and Packaging Exhibition and high-level strategic seminars, and the second session of the China Paper Boutique Packaging Industry Printing and Packaging Fair "in 2009 October 15 to 17 successfully held in Ningbo, show reached an unprecedented scale in more than 15,000 square meters, including Bost, Germany, BHS, Hubei Jingshan, this chang, Xu Heng ironwork, Yiu Branch, Yu-pang, 10000 Alliance, Shengding, Tokyo, Dexter, victory Albert, German steel, Grand View, open up, good friends, music Huang, big pine, sea power, Qatar, Ding Long, Branch Shenglong North Korea and Chang, Kam Sheung Hing, Ji Xing, Eiffel, Shengyuan, etc. are more than 160 domestic and international famous brand supplier exhibitors, fully demonstrated, including pre-press plate-making systems, tile lines, water-based printing slotting machine, slitting machine , cross-cutting machine, rotary die cutting machine, platen die-cutting machine, folder gluer, laminating machine, UV coating machine, Laminator, UV inkjet equipment, carton color box proofing equipment, including a variety of corrugated boxes, color box printing and packaging equipment, has attracted more than 9,600 industry carton color box packaging and printing business professionals to visit the exhibition, access to the industry wide acclaim, becoming in 2009 the second half of the paper packaging industry, the annual communication, making friends, dealing a big gathering.

&第二届中国纸包装工业瓦楞纸箱包装印刷展览会暨高层战略研讨会、第二届中国纸包装工业（宁波彩盒印刷包装展览会暨高层战略研讨会、第二届中国纸包装工业印刷包装精品交易会&于2009年10月15日至17日在宁波成功举办，展会规模达到了空前的15000多平方米，包括博斯特、德国BHS、湖北京山、今昌、旭恒铁工、耀科、誉邦、万联、盛鼎、东京文洪、胜利伟业、德钢、宏景、开拓、佳友、音皇、大松、海力、卡达、鼎龙、科盛隆、朝昌、锦上兴、冀星、埃菲尔、盛源等国内外160多家国内外著名品牌供应商参展，充分展示了包括印前制版系统、瓦线、水性印刷开槽机、纵切机、横切机、圆压圆模切机、平压平模切机、糊盒机、覆膜机、UV 上光机、裱纸机、UV喷墨设备、纸箱彩盒打样设备等在内的各种瓦楞纸箱、彩盒印刷包装设备，吸引了业内9600多名纸箱彩盒包装印刷企业专业人士前去参观，获得业内人士的广泛好评，成为2009年下半年纸包装行业内一年一度的交流、交友、交易的大聚会。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。