切的

The main factors affecting the soil-structure interface behaviors were found experimentally and theoretically, including: 1 the thickness of the interface that is five to six times the average grain size of the soil; 2 the aeolotropy of interface, which is responsible for anisotropic response of the stress-strain response of the interface; 3 two physical states, including crashing and compression of the soil near the structure surface, which govern the stress-strain response of the interface strongly; 4 two shear deformation components due to sliding and constraint of the structure surface relative to the soil respectively, which forms the deformation of the interface; 5 the volumetric strain due to dilatancy, which is found to be composed of a reversible dilatancy component and an irreversible dilatancy component. 4. A unified constitutive model of the interface, based on new elasto-plasticity damage theory, was developed. It was confirmed to be effective for the conditions considering monotonic and cyclic shearing, coupling effect of shear and volumetric strains, evolution of physical state, micro-structure aeolotropy of the soil and the resulting aeolotropy of the interface as well as the three normal boundary conditions stated above. 5. 2D and 3D finite element formulations of the present model were derived and incorporated into the FEM codes. They were applied to the evaluation of practical engineering problems with different typical interfaces between soil and structure. The new model was shown to be reasonable and effective.

确定了粗粒土与结构接触面厚度约为5～6倍的平均粒径，首次揭示了接触面的细观结构异向性以及由此所引起的宏观剪切异向性，发现了在单调和往返剪切荷载作用下土颗粒破碎和剪切压密两种物态变化机制共同支配着接触面力学性质的变化，通过细观分析证实了接触面的变形可分解为一般同时发生的土与结构交界面上的滑移变形以及结构面位移约束范围之内土体本身的剪切变形两部分，观测到接触面受剪时表现出明显的相对法向位移，并可分解为可逆性和不可逆性两个分量；(4)建立了第一个能够统一地描述单调与往返剪切特性、剪应变与体应变耦合特性、细观结构和宏观剪切异向性以及土颗粒破碎等物态变化特性的土与结构接触面弹塑性损伤本构数学模型，并采用多种法向边界条件复杂加载路径的试验成果验证了新模型的合理性和有效性；(5)提出了新模型的二、三维有限元格式并结合实际边值问题进行了应用计算分析，比较了不同接触面本构模型对计算结果的影响，证实了新模型及其有限元格式不仅能够合理地描述土与结构接触面的主要力学特性，还能够较好地反映土体与结构物在接触面处的滑移、脱开等不连续现象。

Five main phenomena were found, firstly, the high plasticity clay could well suitable to large deformation, and had strain-hardening behavior obviously, as well as, the little dilatancy; secondly, there was no correlation between stress paths and shear strength but the stress-strain relations were influenced by stress paths; thirdly, the little stress increment ratio, the higher increase speeding of shear stress and breaking strength under the condition of same initial normal stress; the fourth, the relations between normal strain and normal stress in simple shear test was consistent with which in the single-direction compression test, if there was no dilatancy; the last, the curve of relation between stress ratio and shear strain could be well simulated by hyperbola.

试验结果表明，高塑性粘土能够较好地适应大变形，接触面剪应力与切向应变关系呈剪切硬化型曲线，法向剪胀不明显；接触面剪切强度与应力路径无关，应力应变关系与应力路径密切相关；初始法向应力一定，应力增量比越小，剪应力增长越快，对应的破坏剪应力也越高；无剪胀发生情况下，法向应变与法向应力关系曲线与单向压缩试验具有一致性；应力比与切向应变呈良好的双曲线关系。

And obtained recombinant plasmid PGEM-VP3 by linking prokaryotic expressionby vecto r PGEM-4T-1,and identified by PCR、double restriction enzyme analysis and sequence analysis,the result showed that it's true.

用EcoR I和XhoⅠ双酶切该片段并回收该片段，将此基因片段克隆至相同酶切回收后的PGEM-4T-1原核表达载体中，获得重组质粒PGEM-VP3，经PCR鉴定，限制性内切酶双酶切分析，证实了重组质粒的正确性。

The experimental results show that the impeller with high ratio of circulation to shear is suit to be used in circulation control process, the impeller with low ratio of circulation to shear is suit to be used in shear control process, and the impeller with medium ratio of circulation and shear is suit to be used in circulation and shear co-control process.

实验结果表明，循环与剪切配比高的桨叶适用于循环控制的混合过程，循环与剪切配比低的桨叶适用于剪切控制的混合过程，而循环与剪切配比适中的桨叶适用于循环与剪切共同控制的混合过程。

The shear modulus or modulus of rigidity describes an object's tendency to shear (the deformation of shape at constant volume) when acted upon by opposing forces; it is defined as shear stress over shear strain.

剪切模量或刚度模量，描述了一个物体在反力作用下的受剪切程度；它被定义为剪切应变下的剪切应力。

The reasons why quarter-sawn board of Larch had smaller wet compressing shear strength than flat-sawn board of Larch for repeatedly boiling samples were as follows: first of all, antiswelling efficiency between springwood and summerwood of Larch was different; secondly, pits of Larch had great effect on this aspect.

造成桦木反复煮沸压缩剪切强度径切板的比弦切板的要大的主要原因是在弦切面木射线是被横断的，因此，在弦面上留下射线管胞的孔隙，在反复煮沸时这些孔隙成为了水分的通道，因而使桦木反复煮沸压缩剪切强度径切板的比弦切板的要大。

As the results, the ultra-thin films of 500SN and hexadecane exhibited nonlinear shearing response, which was different from that of the Newtonian fluids in their bulk state.

结果表明：在超薄膜润滑条件下，5 0 0 SN和十六烷均表现出明显的非牛顿剪切响应，即剪切稀释现象；摩擦力幅值随剪切速度的增大急剧上升到最大值，然后下降至某一固定值附近并产生波动；剪切挤压下的临界膜厚小于静态挤压的临界膜厚，剪切运动对吸附层有序结构产生破坏作用

Gravure press is not discharge on down during the mode of production, the per-color business card printing and membership card after harvest cylindrical insertreleased, offline after the subject with a single eyefor a cross-cutting machine rolled business card printing and membership card making for easier and more precise printing long broken sected collection, and then set the broken sected collected leaflets business card printing and membership card making products in spinons convex, indentation, die-cutting, waste, collection, testing, packaging; business card printing and membership card making products for stamping or compact label positioning easy-care, and the increase in the operation, the disadvantage is that the above process, the operation covers an area of large plant, to make more, and sometimes the drum to the materials offline operation in cross-cutting precision |interrupted Zhang superstandard, it will be a large number of categorizing people and a large area of the workspace.

凹印机是采用上料下料不停机的生产方式，进行逐色制卡和会员卡制作后收成卷筒状取下，离线后通过一台识标横切机把卷状制卡和会员卡制作品以单幅印长断切收集，再将断切收集来的单张制卡和会员卡制作品进行棘凸、压痕、模切、剔废、收集、检验、包装；制卡和会员卡制作品如需要烫金或镭射标定位烫，还需增加工序，以上工艺缺点是工序多，占地面积大，使用机台多、人员多，有时卷筒印材离线作业在横切过程中断张精度超标，还需大量的分检人员和大面积的工作空间。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

On the basis of intervene area of two platforms of die cutting machine in dieing process, the model of press was established.

根据模切机的两大平台在模切过程的干涉面积，建立了模切压力模型，利用计算机编程分析得到了模切压力在一个运动周期内的变化情况，为提高模切机的工作性能提供了理论依据。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。