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It can do engraving and cutting on wood and MDF.

它可以做雕刻和切割的木材和中密度纤维板。

This investigation would be able to lay a foundation for enterokinase activity research and farther application of expression products on a large scale.

该粗酶经脱盐后在适宜的缓冲体系中21℃温育过夜,显示出较高的自催化切割活性,为进一步进行重组牛肠激酶活性的研究及应用奠定了基础。

After fermentation and product purification, we got some purified fusion protein SOD-Thyα1. And the Enterokinase digestion of fusion protein was also studied.

经发酵和纯化得到了融合蛋白SOD-Thyα1的纯品,并对融合蛋白的肠激酶切割特性进行了初步研究。

Recombinant hK5 with native N-terminus was generated by enterokinase cleavage and affinity chromatography purification.

经肠激酶切割和纯化,获得了与天然蛋白N-末端相同的hK5重组蛋白。

We also inserted an Enterokinase recognition site between the hSOD gene and the small peptide gene, then we can cleave away the small peptide from fusion protein easily.

在构建时还在小分子多肽与人SOD的连接区插入肠激酶识别位点,这样可以使小分子多肽能从融合蛋白中切割下来。

The resulting was cleavaged with Enterokinase afte lectrelution and dialysis to carries on the inhibiting test and demonstrated activated.

对目的条带电洗脱、透析回收、肠激酶切割后进行抑菌实验,显示有活性。

The fused protein from pPIC9KBC/KM71 yeast strain had the insecticidal activity and could be digested by enterokinase in vifro.

酵母中表达的融合蛋白具有杀虫活性,并且可以被肠激酶进行体外切割

The expressed IL-29 fusion protein was purified by Ni-NTA affinity chromatography and the fusion tag was removed from IL-29 fusion protein by cleavage with enterokinase.

纯化后的融合蛋白经肠激酶切割和回收后,所得目的蛋白(IL-29)纯度大于96%,该蛋白N-端序列与理论值一致,其抗病毒活性与IFN-α2b相当。

The fused insecticidal protein may delay the process of insects?developing resistance. After investigation of the secondary structure and action mode of Bt insecticidal protein and Cowpea trypsin proteinase inhibitor, as well as the action mechanism of insect enterokinase, we devised the fusion gene on the base of maintaining their functions respectively. The method follows: CpTI gene is linked to 3?-end of GFM crylA gene by a nucleotide sequence encoding cleavage site of insect enterokinase.

根据Bt杀虫蛋白Cry1A和豇豆胰蛋白酶抑制剂的高级结构、杀虫机理及昆虫体内肠激酶的作用机理,本着尽量不影响二者功能的原则,设计出了构建Cry1A和CpTI融合杀虫基因CryCI的方案,即将CpTI基因的编码序列连接到GFM Cry1A基因编码区3'端,并在其间用一段编码肠激酶切割位点的序列相连。

The objective of the study was to obtain the gene of bovine enterokinase light chain,which would be used in the cleavage and purification of fusion proteins.The fragment of bovine enterokinase light chain cDNA was obtained by RT-PCR from a sold bovine′s duodenal mucosa, then cloned into the pUCmT cloning vector and sequenced.Compared with the sequence deposited in GenBank,the cloned gene sequence is correct.

为克隆表达牛肠激酶轻链编码基因,以期应用于融合蛋白的切割与纯化,从市售北方黄牛十二指肠组织中提取总RNA,以RT-PCR方法扩增其cDNA片段,将此片段克隆于pUCmT载体中,经过特异性限制性内切酶酶切分析片段正确后,进行全序列分析。

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