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Methods Eukaryotic expression plasmid pCMVCD was constructed , and identified by re2 striction endoenzyme digestion. CD gene was transfected into NSCs from new2born Wistar rats using Lipofec2tamine2000. Positive clones (named NSCs/ CD cells) were screened by G418 presence. 52Fluorocytosine (52FC) ad2ministration of different concentrations were incubated with NSCs/ CD cells. NSCs/ CD cells viability ratios were mea2 sured by MTT assay.

通过构建真核表达质粒pCMVCD ,限制性内切酶消化鉴定后,采用Lipofectamine 2000 脂质体介导法转染新生大鼠室管膜下区神经干细胞(Neural stemcells , NSCs),G418 筛选阳性克隆,加入不同浓度的52氟胞嘧啶(52Flourocytosine , 52FC),MTT比色法测定NSCs的生存率。

The formation of entrenched valley is the mutual effect of tectonic movement and sea level.

下切谷的形成是构造运动和海平面共同作用的结果。

Finally, we establish the exponential decay rates for Naghdi's model under some checkable geometric conditions on the middle surface.

算例的计算结果与文献中给出的实验值吻合较好,并表明壳体的剪切效应在一些情形下会对环肋圆柱壳的稳定性产生一定的影响。

Based on the fact that different metal system hold a same exponential law, we can expect that there must be a physical nature which control the experimental law.

但从某些单晶金属的实验上看,似乎有下面的规律:在滑移系统不变的情况下,在一定的温度范围内,临界切应力和温度呈近指数变化关系。

To begin the months-long process, doctors removed one of Thornton's canine teeth—aka an eyetooth—along with part of the jaw and cut it all down to a shape small enough to replace the cornea.

长达几个月的治疗过程开始了,医生们拔下了桑顿的一颗犬齿——也就是上尖牙,并且取了颌骨的一部分,把颌骨切的足够小,以取代角膜。

On the basis of overwhelming evidence it is now widely accepted that earthquakes are caused by the dynamic spreading of shear rupture on a fault plane.

浅源构造地震是在构造应力作用下地壳内的断层发生快速的剪切破裂而引起的弹性振动。

The specially designed and made flatbed ensures the perfect matches of the beds under different pressure and the smooth conveying of the paper to meet the high requirement of the customers.

经特殊设计的平台保证了在不同压力下上下平台的吻合,纸张顺畅的输送,确保模切的产品能满足客户严格的要求。

A game five days earlier had been abandoned after a floodlight failure with Chelsea cruising 2-0 and only 15 minutes to go.

五天前的比赛中,在切尔西2-0领先而比赛仅剩15分钟的情况下,由于照明灯故障,比赛被取消。

Using this kind of fluxmeter, the heat-flux distribution in a pulverized-coal tangentially-fired test furnace under different operating conditions, the effect of sandwiched-air on the heat-flux and the dependence of the distribution on the coal properties were measured and investigated.

利用该辐射式热流计,作者测试并研究了四角布置切向燃烧锅炉炉膛内在不同燃烧工况下的辐射热负荷分布规律、夹心风的投入与关闭对炉内热负荷分布的影响,以及煤种变化时炉内热负荷分布的变化。

The greatest height of footwall cutting is determined through the theoretical analysis of cut-off grade method and breakeven point method.

通过对边界品位法及盈亏平衡法的理论分析,确定下盘最大切岩高度。

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