切下

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

Its mechanism is downward and backward rotation of the mandible, the retrusion of the lower incisors, the protrusion of the upper incisors and the forward growth of maxilla.

FR-Ⅲ是矫治替牙期前牙反的理想方法之一，其机理是下颌骨向下、向后旋转，舌倾下切牙，唇倾上前牙，促进上颌骨向前生长。

Result］all of enzyme tests were normal.the author could observe the dissolved degeneration of part of sacrospinal muscle fibers,muscle fiber hypertrophy,and a small quantity of inflammatory cell infiltration with a light microscope.focal solution of muscle fiber,the aggregation of mitochondria around the nucleus,the increase of lipid droplet and lysosome in cyte,and the proliferation and differentiation of muscle satellite cell could be observed with an electron microscope.

术中切取竖脊肌标本用于组织病理学观察和透射电镜观察。［结果］各项酶学检验无异常；组织学光镜下观察到竖脊肌纤维部分溶解变性，肌纤维肥大，少量炎性细胞浸润；电镜下观察到肌纤维灶状溶解，核周线粒体聚集，胞内脂滴、溶酶体增多，肌卫星细胞增殖分化。

The author could observe the dissolved degeneration of part of sacrospinal muscle fibers, muscle fiber hypertrophy, and a small quantity of inflammatory cell infiltration with a light, microscope. Focal solution of muscle fiber, the aggregation of mitochondria around the nucleus, the increase of lipid droplet and lysosome in cyle, and the proliferation and differentiation of muscle satellite cell could be observed with an electron microscope. Pathogenesis of chronic lumboscaral compartment syndrome may be as followed.

术中切取竖脊肌标本用于组织病理学观察和透射电镜观察［结果］各项酶学检验无异常；组织学光镜下观察到竖脊肌纤维部分溶解变性，肌纤维肥大，少量炎性细胞浸润；电镜下观察到肌纤维灯状溶解，核周线粒体聚集，胞内脂滴、溶酶体增多，肌卫星细胞增殖分化。

The decreased threshold shear velocity and unsaturated sand flows at the stoss slope of the dunes resulted the erosion at the toe of the dune. Under the medium and low drifting-sand winds the sands being transported have divergence with that on the surface, the mean grain size of transported sands is lower than that on the surface and the sand transporting is a stochastic process.

起动剪切速度减小与不饱和风沙流是沙物质在横向沙丘迎风坡坡脚不能产生沉积的主要原因；再者，平均风速并不能完全反映野外实际状态下气流的性质，在低－中强度的风沙活动过程中，被输送的沙物质的粒度组成与下垫面物质有明显的差异。

The total effective rate was 90%.Conclusion Indicated by ECoG, combined resection with corpus callosotomy or multiple subpial transection is an effective method for FLE.

在皮层电极指导下采用痫灶切除并联合应用胼胝体切开或多软膜下横切是治疗额叶癫痫的有效方法。

Pathologic histology with vaginoscope is a simple and effective method for diagnosing CIN, conization of cervix can remedy the defect of the pathologic histology with vaginoscope and shows a therapeutical effect.

阴道镜下活检是诊断CIN的一种简单而有效的方法，宫颈锥切术可以弥补阴道镜下活检的缺陷，且有治疗作用。

Pathologic histology with vaginoscope is a simple and effective method for diagnosing CIN, conization of cervix can remedy the defect of the pathologic histology with vaginoscope and shows a therapeutical effect.

阴道镜下活检是诊断CIN的一种简单而有效的方法，宫颈锥切术可以弥补阴道镜下活检的缺陷，且有作用。

Pathologic histology with vaginoscope is a simple and effective method for diagnosing CIN, conization of cervix can remedy the defect of the pathologic histology with vaginoscope and shows a therapeutical effect.

阴道镜下活检是诊断CIN的一种简单而有效的方法，宫颈锥切术可以弥补阴道镜下活检的缺陷，且有治疗作用。中国论文联盟http：//www.lwlm.com

Pathologic histology with vaginoscope is a simple and effective method for diagnosing CIN, conization of cervix can remedy the defect of the pathologic histology with vaginoscope and shows a therapeutical effect.

阴道镜下活检是诊断CIN的一种简单而有效的方法，宫颈锥切术可以弥补阴道镜下活检的缺陷，且有治疗作用。中国诺宇综合网http：//www.siaaa.com

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。