切下

With primers designed according to sequence(AY052417) of diapause pupae of oriental tobacco budworm registered on GenBank, a double-strand cDNA fragment of DH gene was obtained from RT-PCR amplification and was further cloned into pGEM-T Easy vector.

根据GenBank发表序列(AY052417)设计引物，以烟夜蛾(Helicoverpa assulta Guenée)滞育蛹为材料，利用RT-PCR技术扩增到滞育激素(diapause hormone，DH)cDNA，并通过TA克隆的方法将其克隆至pGEM-T Easy载体上，对目的基因进行测序，并利用设计的酶切位点将目的基因从T载体上切下。

In this research we obtained the banana ACO gene with about 1.2kb length by cuffing from the cloning vector pUCm-ACO with restriction endonucleases Xba Ⅰ and Sac Ⅰ, and then directionally linked the gene to the plasmid vector pBⅠ-121 in the same endonucleases digestion sites, finally we got the construct of plant expression vector pBI-aACO.

用限制性内切酶Xba Ⅰ和sac Ⅰ从克隆载体pUCm-ACO上切下约1.2kb的香蕉ACO基因，将其定向连接在经相同酶切的质粒载体pBⅠ-121上，构建成植物表达载体pBⅠ-aACO。

In our experiment, the GADes gene from rat brain was cloned and expressed in E.coli, so as to make the recombinant GADes produced by gene technology for clinical application and further study on the relation between GAD and type 1 diabetes mellitus.Firstly, with the pUClS/GADes recombinant plasmid as template, the full-length encoding sequence of GADes was amplified by PCR, and cloned into pGEM-T vector. Secondly, the target gene was cut by EcoRI and inserted into restriction sites of pET42a vector for expression.

本研究首先用PCR方法，以pUC18/GAD_(65)为模板，扩增目的基因，克隆到pGEM-T载体中，构建pGEM-T/GAD_(65)重组质粒，然后用EcoRI酶切，切下的目的基因片段插入pET42a融合型表达载体中，经酶切鉴定和测序，证实插入方向、读码框架正确；重组表达质粒转化大肠杆菌BL21(DE_3)中诱导表达，表达产物经亲和层析纯化后获得了融合蛋白GST-GAD_(65)。

Expression of Bilirubin Oxidase cDNA from Myrothecium Verrucaria in E. coliThe 1. 6kb bilirubin oxidase cDNA from Myrothecium Verrucaria was excised from plasmid pGEM-T/BOX, and inserted into expression vector, pET-3a which were under the control of a T7 promoter.

用限制性内切酶将1.6kb疣疱漆斑菌胆红素氧化酶cDNA从质粒pGEM-T／BOX切下，插入到表达质粒pET-3a的T7启动子下游，构建成疣疱漆斑菌胆红素氧化酶表达质粒pET-3a／BOX。

Bring a medium pot of water to a boil; add asparagus (make sure water covers asparagus). Cook 5 minutes. Drain, reserving 1 cup asparagus water. Remove 16 asparagus tips; cut in half lengthwise; set aside.

拿一个罐子装水煮，放入芦笋，煮五分钟后，将水倒出，储存一杯芦笋水，切下16个芦笋顶部，切成两段，放在一旁。

One use is a short-cut when making a Star Cane - cut lots of stars using a star cutter, stack and compress, then do the same with the background colour, and cut one side open to insert the centre star shape Kemper Brand cutters are also useful - they make a range of different size cutters with plungers to help remove clay plug.

例如要做一个星形的软陶棍，可以用星形的模具切下多个星形的胚子，将他们叠在一起压合，在另一个用来做背景的陶泥上用同样的方法切出星形镂空的胚子，再把他们叠合在一起，然后切开一边把先前做好的星形胚子放入再封口即成。

The working principle: 1, with the membrane machine, Slitter linkage: in the production process, from bits and pieces of the film strip was placed in the feed granulating machine body, the binder device, irregular bits and pieces have been pressure neatly into the pattern with the ribbon, and then by the high-speed rotary cutting blade alloy materials for the flaky particles, instantaneous complete recovery.

工作原理：1、与制膜机、分切机联动：在生产过程中，排出的带状薄膜边角料立即被送入造粒机的进料机构，经压料装置，不规则的边角料被压成整齐带花纹的带状，再由高速旋转合金刀片裁切为片状颗粒材料，回收瞬间完成。2、单独使用：将分切下的边料有序的卷到纸芯上，成卷地送到造粒机进行造粒。

The most suitable explants for in vitro growth were shoot-tips and stem segments from sprouting cutting which were plugged in water or in vermiculite under weak light more than 24 h.

最适合的外植体是新切下的枝条插入含有蛭石和充足水的营养钵中24h以上，使其在弱光下迅速长出嫩枝的茎切段和茎尖。

Results The recombinant plasmid pSNAV2.0-TK was verified byPCR and restricton endonuclease. The coat protein of AAV was detectedby SDS-PAGE method, the purity of rAAV2/HSV-TK was＞98% byHPLC method and the titre of rAAV2/HSV-TK was 1×10~(12)v.g./ml byDNA dot blot hybridization.

结果：重组质粒pSNAV2.0-TK PCR扩增的片段大小和酶切鉴定所切下的片段与预计结果一致；SDS-PAGE法检测到三条特征性的AAV外壳蛋白条带，HPLC法检测rAAV2／HSV-TK的纯度＞98％；用DNA斑点杂交方法检测rAAV2／HSV-TK的滴度为1×10~(12)v.g。

The sectional surface of the cut products are quite plane and smooth, it can save raw material.

切下的制品断面平滑，节约制品原料，对制品的切制可在生产线上随时同步进行，也可不在生产线上随时随样切割，切割平稳、无噪音。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了