切下

A branch cut off from the adjacent branch must of necessity be cut off from the whole tree also.

从邻枝上切下的一根枝条必定也是从整个树上切下的。

And then adding NAA at low concentration in MS was used to give rise to roots. We also found that lower level of hormone could control effectively browninng and vitrifaction during the culture and G1 n (6mg/L) and AgNOj (2mg/L) supplemented in shoot induction media could improve the shoot information rates apparently (about90%).The whole period of plant regeneration from leaflets of peanut could be divided as five steps: germination - shoot induction -shoot elongation-rooting- tranplant.

用1／2MS培养基萌发花生种子，9-10d后，从无菌花生苗上切取幼嫩叶片中部为外植体。2500Lux光照和27±1℃条件下，在诱芽培养基(MS+BA3mg／L+NAA0.8mg／L+AgNO_32mg／L十Gln 6mg／L)培养12-14d即可观察到明显芽点或瘤状突起，较前人报道的培养时间大大缩短了，4w后芽点进一步发育成丛生芽，芽诱导率达90.2％，每个外植体平均产9个丛尘芽，然后转至培养基MS+BA3 mg／L+AgNO_32 mg／L上诱导芽的伸长，3-4w后可长至3-4cm，切下带有2-3片叶片的幼芽移至生根培养基(MS+NAA0.8mg／L+AgNO_32mg／L)，1w后切口处可见白色不定根形成。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

In the study of defect problems in elastic media, first, the uniform stress fields within a three-phase anisotropic elliptic inclusion in anti-plane shear is discussed; second, an exact solution is given for an edge dislocation in a three-phase composite cylinder model with a sliding interface; finally, an analytic solution is derived for two circular inclusions with circumferentially inhomogeneously imperfect interfaces interacting with a circular Eshelby inclusion in anti-plane shear.

在对弹性介质缺陷体问题的讨论中，我们首先探讨了面外剪切变形下各向异性三相椭圆夹杂中均匀应力场；其次研究了具有滑动界面的三相圆柱复合模型中的刃型位错问题；最后分析了面外剪切下具有环向非均匀界面的两个圆柱异相夹杂与一个圆柱Eshelby夹杂的相互作用问题。

The function of cutting knife was to cut bamboo strands from a bamboo piece. Before they were cut off, a scoring-knife was used to precut a deep groove in the bamboo peice to section the strands.

切削刀片的作用是将竹刨花从竹毛坯上切下，但在竹刨花被切下之前已被定长划线刀片在竹材表面划出一定深度的槽沟，切断竹材纤维，使被切削下的竹刨花自然形成分段状态。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

According to this phenomenon, we analyze the original circuit structure of unreasonable, in the original circuit has added a intermediate relay (5J), a limit switch, and take advantage of the original paper cutting, the unused in the buttons on the normally closed contact, specifying the control switch some of cam shape change, so that you solve the Cutter knife to cut the ?

根据这种现象，我们分析了原电路的不合理结构，在原电路中添加了一只中间继电器（5J）、一只行程开关，并利用了原切纸按钮中未用上的常闭触点，同时将控制行程开关的凸轮形状稍加改变，这样就彻底解决了切纸刀切下后自动复位难的问题，使切纸速度达到了要求的次数。

Full length LMP2A cDNA was firstly incised from pGEM-T-LMP2A with EcoR Ⅰ, Sma Ⅰ digestion, and then inserted into eucaryote. expression plasmid pCIcc controlled by CMV promoter. The CMV-LMP2A-SV40 expression unit was digested by ClaI, and inserted into E1-substituted adenovirus vector pAx1cw. Then the LMP2A recombinant adenovirus vector was cotransfected into 293 cells together with EcoT22I digested Ad5-TPC.

将带有LMP2A cDNA的重组质粒pGEM-T-LMP2A用EcoR Ⅰ、Sma Ⅰ双酶切下LMP2A cDNA，并将其插入含同样酶切位点的真核表达质粒pCIcc中，使其受控于CMV启动子下；用ClaI切下CMV-LMP2A-SV40表达单元，插入E1、E3区替代的腺病毒载体pAX1CW，选择正确的克隆pAX1CW-LMP2A与Ad5 DNA-末端肽复合体共转染293细胞，通过同源重组获得复制缺陷型的重组腺病毒(Ad5-LMP2A)。

He sliced his finger by accident when cutting vegetables.

他切菜时不小心割破了手指。v。切，切下，切成薄片

Eats the meat: Starts from the left side to cut, stops by the fork from left side the meat, again uses the knife to cut open along fork right flank the meat, like cuts the meat is unable under a stuttering, but straight takes over the use of the knife to cut small somewhat again, cuts open a just size the meat, then delivers in directly the population by the fork.

吃肉：从左边开始切，以叉子从左侧将肉叉住，再用刀沿着叉子的右侧将肉切开，如切下的肉无法一口吃下，可直接用刀子再切小一些，切开刚好一口大小的肉，然后直接以叉子送人口中。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了