切

Diagram D, JDBC Walk the defender down to the block, plant the inside foot and quickly make the V-cut back out. This time the defender is ready and is in front you, over-playing the pass. So now plant the outside foot, and make a quick back-cut.

与防守者一起游走至阻区，停住后立刻做一个V字切出来，此时防守者已经做好准备，在你前方绕前阻绝，此时你应将外侧的那只脚停住，并作一个迅速的后切。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

The dish washer includes one washer body with inside washing tank, rotary door in the front of the washer body, and one sealing latch set between the washer body and the door to raise the sealing property of the door. The sealing latch includes one hook unit set inside the hole on the washer body side, one sealing unit exposed outside the washer body and contacting closely to one side of the door in close state, one central slot for telescoping the hook unit, and one expansion joint in the joint part to avoid concentration of stress.

本发明包括洗碗机内部具备了洗涤槽的本体，设置在本体的前面转动的门，设置在本体前面和门之间，当关闭门时提高门和本体前面的封闭性的碰销；其中碰销包括：在本体前面一侧形成的插入孔中设置的挂钩部，露出于本体外部，在门关闭时，紧贴门一侧面的密封部，为了使挂钩在插入孔内直径方向伸缩，而在其中央部位形成的切缝，其特征在于密封部上形成了与切缝相通，防止挂钩伸缩时应力集中到挂钩部和密封部之间结合部位上的伸缩槽。

Based on the analysis of the experimental results of flue gas temperature distribution in horizontal flue in a 300MW pulverized coal tangentially fired boiler at variable load, the characteristics of the flue gas temperature distribution in horizontal flue of this boiler at variable load are summarized in this paper.

通过对某 3 0 0 MW四角布置切圆燃烧锅炉在变负荷工况下水平烟道内烟温分布的现场试验结果的分析，指出了四角布置切圆燃烧锅炉水平烟道内烟温分布的特点及其随负荷变化的规律。

She said. The programme also reveals that Blair calls his wife "the bolshie Scouser," a reference to her Liverpool, north-west England roots and often outspoken views.

另据节目曝光，布莱尔称呼切丽为&利物浦的布尔什维克&，因为原籍为英格兰西北部利物浦的切丽常常直言不讳。

The programme also reveals that Blair calls his wife "the bolshie Scouser," a reference to her Liverpool, north-west England roots and often outspoken views.

另据节目曝光，布莱尔称呼切丽为&利物浦的布尔什维克&，因为原籍为英格兰西北部利物浦的切丽常常直言不讳。

It also has an off brand-new Heidelberg Speedmaster four-color Second Taiwan, monochrome printer, the book version of BBJ machine, polishing machine, laminating machine, rolling and cutting machine, cutter and so on, more than 10 different models of special Taiwan printingè±.

更拥有全新海德堡速霸对开四色二台、单色印刷机、书版BBJ机，上光机、覆膜机，轧切机，切纸机等，十多台不同型号的专用印刷设备。

Product main business: 1, 1400 ~ 2500mm, single, three, five, seven corrugated cardboard production line 2, 2200 ~ 3500mm, double, three, four, five-color printing slotting die-cutting machine three, 2400 1200mm automatic visco-box machine , semi-automatic machine stick me four, all kinds of specifications±sub-machine semi-automatic paper isobar nail box machine, paper balers, etc. Friday, 800 ~ 1620 mm automatic platen die-cutting the six-ping, 1100 ~ 1450mm automatic paper mounting machines, seven semi-automatic paper mounting machine, a variety of bundlers, eight winding machine, all kinds of specifications, types of testing equipment carton¨Buck Shanghai Machinery Co., Ltd. of China's packaging industry and the packaging machinery market very well.

主要经营产品：一、1400~2500mm ，单、三、五、七层瓦楞纸板生产线二、2200~3500mm，双、三、四、五色印刷开槽模切机三、2400×1200mm 全自动粘箱机、半自动粘箱机四、各种规格的薄刀分纸压线机半自动钉箱机、废纸打包机等五、800~1620 mm 全自动平压平模切六、1100~1450mm全自动裱纸机、半自动裱纸机七、各种规格的捆扎机、缠绕机八、各种规格、类型纸箱检测设备上海川斯霸克机械有限公司对中国包装行业及包装机械市场非常了解。

Friends of Grace paper products company nanshan existing tri-color, two sets of two-color watermark machine production lines, production machinery configured carton including Taiwan detachable three-color watermark machine, MY --- 2500-type rotary slotted angle cutter, FY ---2500 points±paper type pressure-ray machine, ML --- 1300-type die-cutting machine, DX --- 1300 nail box machines that can contract a variety of specifications of high, middle and low-grade, single, double corrugated boxes, carton manufacturing, at present our factory has the production of 20 million square meters in three of five criteria for corrugated box production capacity, 50,000 cartons per day of production scale.

友宏公司南山纸品厂现有三色、双色水印机两套生产线，配置的纸箱生产机械包括台湾可分离式三色水印机、MY---2500型轮转开槽切角机、FY---2500型薄刀分纸压线机、ML---1300型模切机、DX---1300钉箱机等设备，能承揽各种规格的高、中、低档，单、双瓦楞纸箱、纸盒的制造，目前我厂已具备年生产2000万平方米三层、五层标准瓦楞纸箱的生产能力，每日5万只纸箱的生产规模。

To know that the steel ring can be as a framework, this should be the outline for the chest and then give it a load bearing capacity, but if the pressure is too small in the chest that will be cut as tofu, like the original belonging to the chest of fat to it Cut off the breast will be getting smaller.

要知道，钢圈可就象个框架一样，本应该是适合胸部轮廓然后给它一个承托力的，但如果过小压在胸部那，就会象切豆腐一样把原属于胸部的脂肪给它切出去，乳房就会越变越小了。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。