切

A human transforming growth 〓 cDNA was inserte in reverse orientation into the retroviral vector, pLXSN. Restriction en zyme analysis was used to confirm the antisense orientation of 〓〓 in the pLXSN vector from individual transfomants.

将人的转化生长因子-〓cDNA（1467bp）反向插入逆转录病毒载体pLXSN，筛选转化菌，挑取抗性克隆，用限制性内切酶酶切鉴定重组子。

It is difficult to play Chelsea 11 against 11 so you can imagine 10 against 10,' he added with reference to the second-half red card for defender Eric Abidal.

我们不能凭空造出来，我希望切尔西走的更远，但是他们没将机会把握住，对于10打11，而且是面对的是切尔西，这很难，你能想象。

DESCRIPTION】the ingredients of the product comes from abyssopelagic fishes of special pure and clean sea areas, adopting Limited Enzymic Degraded Technology, a self-owned intellectual property, that is using compound bioenzyme preparations at a low temperature to extract active ingredients through a special low-temperature extraction process and specific sheared protein to ensure the bioactivity of Collagen.

本品原料来自纯净、无污染的特定海域中的深海鱼类，采用具有自主知识产权的限制性酶切制备多肽技术（Limited Enzymic Degraded Technology），在低温下使用复合生物酶制剂，通过特殊低温萃取工艺提取有效成分，通过特异性的剪切蛋白质，保证了胶原蛋白原有的生物活性，并将原分子量为30万左右的胶原蛋白分子降解成3500左右的胶原蛋白多肽（约含30个左右的氨基酸残基），其分子长度降至30—50nm，集中度达到99.5%以上，呈白色或淡黄色，无异味，无杂物，不添加任何激素和化学物质，没有任何毒副作用。

After a couplet of "O sole mio", Addy said : You can go back to play soccer.

海因切就唱起了《我的太阳》。只唱了几句，阿迪就告诉海因切：可以了。

After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

选择我国流行优势菌株副猪嗜血杆菌血清5型地方株为研究对象，提取细菌基因组DNA，用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切，回收大小为1～5Kb的DNA片段，将其连接入原核表达载体pET-28a-c，最后转化宿主菌，结果成功地构建了基因表达文库，为后续的基因筛选工作奠定基础；另外，本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计，以副猪嗜血杆菌血清5型地方菌株的基因组DNA为模板，进行PCR扩增反应，结果表明成功地获得两个新基因：23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸)，并进一步做了不同物种之间的分子系统发育分析，构建了基于23S rRNA基因的邻接法系统发育树，阐明了副猪嗜血杆菌与其它菌种的分子进化关系。

In equipment investment, a 台平 applanation die-cutting prices will be enough to buy more than one rotary die-cutting machine.

在摆设投资方面，一台陡压陡模切机的代价足以采办众台圆压圆模切机。

Business card printing and membership card making of aluminum foil in packing factory next processing to be embossed/triadha, rats'semitransected, applanation, die-cutting or trimming made the subject of the request by the customer.

制卡和会员卡制作后的卷筒铝箔在包装厂后面的几道工序中要经过压纹/扎孔、横断、压平、模切或冲切制成客户要求的标形。

Line Rotary die-cutting on cigarette packs such high-end application has taken shape in rrepublic, a circle applanation process with very little rrepublic is mainly used for large scale of products.

连线圆压圆模切在烟包等高端领域的应用已经初具规模。而圆压平工艺用得很少。主要用于大尺寸产品的模切。

Methods With polymerase chain reaction-single-strand conformation polymorphism combining argentation and glue retrieval, DNA sequencing, and restriction fragment length polymorphism, the SNP of the 50 exons in all the coding regions of ABCA1 gene was detected in 112 patients with established diagnosis of coronary heart disease.

通过聚合酶链反应－单链构象多态性分析结合银染后胶回收、DNA测序和限制性内切酶酶切分析方法检测112例明确冠心病诊断患者的ABCA1基因全部编码区50个外显子的SNP。

Adhesive forces and contact area between arolium and the target surface were measured when Lycorma delicatula walked on the level and slided on the vertical surface. A model of peeling theory was put forward to give relationship between contact area and adhesive forces.

测量了斑衣蜡蝉在切向和法向两种状态下的抓附力以及在切向和法向两种状态下抓附时中垫与接触面的接触面积，分析了接触面积和力的关系。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。