切

RESULTS:①Enzymolysis and nucleotide sequence analysis of recombinant plasmids showed that two VEGF-C siRNA eukaryotic expression vectors were constructed successfully.

结果：①重组质粒的酶切、核苷酸序列分析结果：经酶切和测序鉴定证实成功构建了血管内皮生长因子C小干扰RNA表达载体，建立了稳定转染细胞株。

Methods The LPL cDNA was isolated from the human epiploon adipose tissue by means of RT-PCR. The LPL cDNA was ligated into the pcDNA3.1Zeo. The recombinant pcDNA3.1Zeo-LPL cDNA was identified by endonucleases, PCR and DNA sequencing. COS-1 cells were transfected with the recombinant LPL gene plasmid using Lipofectamine 20001M The LPL mass in cells and the culture medium were determined by a Markit-M LPL Kit. Spectrophotometry was used to measure the LPL activity.

采用RT-PCR法从人大网膜脂肪组织获取LPL cDNA基因，以pcDNA3.1Zeo质粒作为载体，运用基因克隆技术构建野生型人LPL cDNA重组质粒，限制性内切酶酶切、PCR以及双向测序鉴定重组质粒；运用Lipofectamine 2000将重组pcDNA3.1 Zeo-LPL cDNA质粒导入COS-1细胞，RT-PCR法检测LPL mRNA，ELISA法和比色法分别检测细胞裂解液及细胞培养基中LPL浓度及活性。

Episiotomy cuts through muscle tissue as well as skin, which often lead to urinary incontinence.

侧切手术是直接切过肌肉和皮肤，会导致小便失禁症。

Tears actually heal better than episiotomies. With proper perineal support, both tearing and episiotomy can be avoided.

自然撕裂其实比会阴侧切手术更好，在合适的医疗手段的帮助下，侧切和撕裂都是可以避免的。

It was found that there were 36 distinct differences in 184 protein spots. In-gel digestion and liquid chromatography mass spectrometry C LC-MS were made to analyze the protein SSP2801, and Mascot software was applied to identify the protein. The results showed the protein was large subunit of Rubisco, and its content in yellow mutant decreased distinctly, only about 26% of the comparison. Maybe it has relation to etiolation.

切耿其中差异最为明显的SSP2801位点蛋白进行胶内酶切、LC-MS分析，并应用Mascot软件检索鉴定，结果表明该蛋白组分为RUBP羧化酶的大亚基，其在突变体中含量明显减少，仅为对照的26％，表明突变体叶色黄化与RUBP羧化酶大亚基含量减少也有关系。

In this paper chip shapes were recognized by using of radbas neural network. We put forward area ratio feature, Euler number feature, etc. geometry feature of chip shape image and thing of those features as inputting vector of neural network. Adopt radbas neural network and training the network using RLS.

研究了径向基函数神经网络在硬质合金刀具切屑形态图像识别中的应用，提出了面积比、欧拉数、分散度等硬质合金刀具切屑形态图像的几何特征，以上述特征作为神经网络的输入矢量，利用径向基函数网络，采用了递推最小二乘法训练该网络。

Under the same nominal bond strength, newly devised model achieved much evener stress distribution with the peak stress level positioned in the middle area of upper edge.

在相同表观强度的剪切加载中发现：新建的方形均匀截面模型在应力分布上明显优于传统的剪切测试模型，模型的上边缘应力分布平滑连续且应力最大值位于边缘中央区域。

It is also presented that shear modulus of unsaturated cohesive soils varies with water content or saturation degree evidentially, so notable change of share wave velocity is aroused.

分析中还指出非饱和粘性土的剪切模量随含水量的变化较大，从而会使剪切波速度发生较大的改变。

In exterior differentiation, one studies tensors, exterior algebra, tangent bundle, tangent vector fields, one-parameter groups action on a manifold, Frobenius's theorem, tensors fields, exterior differential form, integration on manifolds, Stokes's theorem etc.

外微分部分主要涉及外代数、切丛，光滑切向量场，单参数变换群，光滑张量场，外微分形式，Frobenius定理，外微分形式的积分，Stokes定理及其应用等。

The three-ply, five-ply, seven-ply, and multi-function corrugated cardboard production line mainly consist of the following equipment; Mill Roll Stand, Surface Paper Preheater , Single Facer, Conveying Span Bridge, Glue Application Machine, Double Facer, Slitter Scorer, Cut-off, Conveyer and Stacker, Heating System, Air compression system and Glue Making System, etc.

主要由纸架、面纸预热器、单面瓦楞机、天桥输送机、涂胶机、双面机、纵切压线机、横切机、堆码机以及加热系统、空气压缩系统、制胶系统、电气控制系统等主要设备组成。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。