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Fig 1 Negative control Female umbilical cord sample has no Y-specific signal after in situ hybridization with the biotinylated Y-repeated sequence DNA probe PY3.4 Fig 2 Positive control Flow-sorted male umbilical cord cells hybridized to Y-specific DNA probe PY3.4,Every cell contains a Y-specific signal Fig 3 Fetal cells sorted from maternal blood Flow-sorted cells from a pregnant woman at 20 weeks of gestation hybridized to Y-specific DNA probe PY3.4,containing a Y-specific signal Fig 4 The result of polymerase chain reaction Lane 1:male umbilical cord NRBCs sorted by FITC-conjugated anti-monoclonal glycophorin A;Lane 2:sample of 2;Lane 3:male umbilical cord NRBCs sorted by FITC-conjugated anti-CD36;Lanes 4-5:samples of 1 and 3;Lane 6:50 cells of male;Lane 7:5 cells of male;Lane 8:200ng male DNA(positive+control);Lane 9:nonpregnant female DNA(negative+control);Lane 10:ΦX174 HaeⅢ Maker,271bp:amplified band of Y-specific gene SRY;383bp:amplified band of human β-globin gene

图1 阴性对照女性脐带血标本,经与生物素标记的Y-染色体重复序列DNA探针原位杂交后未见Y-染色体特异信号图2 阳性对照分选的男性脐血细胞经与Y-特异DNA探针杂交后,每一细胞都含有Y-染色体特异信号图3 母体外周血中分选的胎儿细胞从一位妊娠20周的孕妇外周血中分选出的细胞经与Y-特异DNA探针杂交细胞中含有Y-染色体特异信号图4 聚合酶链反应结果 1:GPA-FITC单抗分选男胎脐血NRBCs;2:2号标本;3:CD36-FITC单抗分选男胎脐血NRBCs;4、5:1、3号标本;6:50个男性细胞标本;7:5个男性细胞标本;8:200ng男性DNA;9:未孕女性DNA;10:ΦX174 HaeⅢ标准,271bp:为SRY基因扩增带;383bp:为人β-珠蛋白基因扩增带内参照

In single-cell transplants, an average of 27% of unselected melanoma cells from four different patients formed tumours.

在单细胞移植中,来自四个不同患者未分选的黑色素瘤细胞中平均27 %会形成肿瘤。

ABSTRACT This paper summarized the study and application state of the technology on coal dry beneficiation with common fiuidized bed and vibration fluidized bed , elaborated the theoretical basis that VFB can beneficiate fine coal.

本文综述了普通流化床干法选煤技术及振动流化床技术应用与研究的现状,阐述了振动流化床分选细粒煤的理论依据。研究了适合于细粒煤分选的振动流化。。。

Aiming at the key issue to solve urgently in signal processing of electronic warfare, system model and algorithms for signal sorting of unknown radar emitter in complex environments are studied systematically and exploringly in this dissertation.

针对雷达对抗信号处理研究工作中迫切需要解决的关键理论问题,本论文对复杂环境下未知雷达辐射源信号分选的体系结构和新方法进行了探索性和系统性的研究,获得了如下研究成果:1提出一种新的未知辐射源雷达信号分选模型结构。

The development and application of high-throughput sorting methods has significantly improved the efficiency of cell sorting and expanded the range of screening. It resultantly increased the probability of getting the higher produce cell line.

高通量分选方法的发展和应用,提高了转染后细胞的分选效率、扩大了细胞分选的范围,从而显著地提高了获得外源基因高表达细胞株的几率。

Three types of keratinocytes were studied: the SP, the main population, and the unsorted initial population.

本研究中研究了三群细胞:侧群细胞、主群细胞、以及未分选的细胞群。

The industrial practice indicate that the circle centrifugal flotation cell is efficient in coal flotation,especially in fine particle separation,and has the advantage of high flotation_speed,sharper separation and high productivity of per cubic co...

工业试验结果表明:圆形离心浮选机对煤泥特别是细煤泥的浮选效果好,具有浮选速度快,分选选择性好,单位容积处理能力大等优点,是实现微细矿物分选的有效设备

In order to satisfy the demand of fruit integrative grading by appearance quality and weight, a weighing module for fruit integrative grader was designed.

为满足水果外观品质和质量综合分选的实际需要,设计了水果机器视觉质量综合分选机的称重模块。

NDT300 series gradation system for sucker rods and tubings developed by Jianghan Machinery Researd Institute can solve the problem of online gradation of rods and tubings.

大港油田从江汉机械研究所引进了NDT3 0 0系列杆管级别分选系统,有效地解决了杆管级别在线分选的问题。

The facilities selection and process system for steam coal separation and steam coal blending problem are discussed and analyzed in this paper.

阐述并分析了动力煤分选的设备选择,工艺系统及动力配煤问

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。

Closes the eye, the deep breathing, all no longer are the dreams as if......

关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。