分裂间期

Objective:To improve the detecting resolution of genes on interphase cells for assisting in clinical diagnosis.

目的为了提高细胞分裂间期细胞核基因检测的分辨率，以协助临床诊断。

PinX1 is a potent telomerase inhibitor in interphase; however, its function in mitosis is not well documented.

PinX1是一个潜在的间期端粒酶抑制因子，它在细胞有丝分裂期中的报道很少。

A dramatic remodeling of interpolar microtubules into an organized central spindle between the separating chromatids is required for the initiation and execution of cytokinesis.

因此为了保证有丝分裂期核内事件的准确进行，在间期，对相关的重要蛋白质的核内稳定性的准确调控尤其重要。

Interval observations under thefluorescent microscop were performed with excition waves of 470—490 nm.The results displayed that GFP genes were expressed effectively in E.octocarinatus, and GFP was distributed unifimly around macronuclearand diffused to the entire cell gradually. The chromosomes can be kept inthe macronucleus for 90h.

用优化的脂质体转化方法将含有人工染色体的重组质粒pBTub-tel2转化到处于有性生殖分裂间期阶段的游仆虫细胞中，结果表明，GFP基因在游仆虫细胞中得以高效表达，36-48小时绿色荧光均匀分布在细胞核周围，逐渐扩散到整个细胞，染色体能够在大核中保持约90小时。

More cleavage phases can be counted by FISH than G-band dyeing on the diagnosis of mosaicism kyrotype, even many intermediate stage cells can be analyzed by FISH procedure. Time was saved and more precise results can be found.

与常规染色体核型分析方法比较，FISH技术检测嵌合体核型能够观察到更多的分裂相，还可分析核膜完整染色体未分散的间期细胞，计数更多的细胞，为临床提供更准确的数据，且省时。

It was showed that throughout the nuclear division, nuclear membrane did not disintegrate,lamina always existed; But only one lamin peptide (similar to lamin B in higher eukaryotes) was expressed during division,ant its expression quantity changed according to the progress of division: in interphase, lamin was constant; when entering G1 phase, lamin increased sharply; then, increased slowly untill division finished.

结果表明：在整个核分裂过程中，核膜不崩解，核纤层结构一直存在；但随着分裂的进展，始终只有一种成分（类似于高等生物lamin B ）的表达；其表达量随着分裂时相的推移而变化：间期时，比较恒定，进入G1期时明显增加；以后不断略有增加，直到分裂完成。

Results: hALP distributed predominantly in the nucleoli of HeLa and Saos2 cells, and colocalized with rDNA.

结果：hALP在HeLa及Saos2细胞分裂间期主要定位于核仁，并与rDNA有共定位。

LSD1 localizes on centrosomes during interphase and spindle poles during mitosis.

LSD1在细胞间期能够定位在中心体上，在细胞有丝分裂期定位在纺锤体两极。

For transgenic mice,98%~100% of metaphases and 85%~94% of interphases showed hybridization signal.For negative control mice,100% of metaphases and 95%~96% of interphases showed no hybridization signal.

阳性转基因小鼠98%~100%的中期分裂相，85%~94%的间期核出现杂交信号；阴性对照小鼠100%的中期分裂相、95%~96%的间期核未出现杂交信号。

C-banding analysis of satellites in 9 accessions of Ae.tauschii native to China was carried out with C-banded miotic interphases,prophases and metaphases,The results showed that the c-banded heterochromatin of satellites of Ae.tauschii did not change in size during the whole miotic stage and novariaton was observed among plants and cells within a accession,while polymorphism in c-banded satellites between different accessions was found.

5D染色体是节节麦整个染色体组中唯一的1对随体染色体，其随体位于5D短臂的末端。对来源于中国的9个不同的节节麦居群的吉姆萨C带分析表明：同一份节节麦居群内不同植株或不同细胞，这个端部随体在整个有丝分裂前期、中期和间期都很稳定，表现为大小、强弱一致的端部特征带。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。