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The acquisition of friable calli and the establishment of the cell suspension cultures of tobacco NC89 leaf were studied.

研究表明细胞分裂素与生长素浓度的比值高时,有利于芽的分化,相反细胞分裂素与生长素浓度的比值低时,有利于根的分化。

The determination of cytokinin levels is a primary and basic approach in the cytokinin studies. The Amaranthus betacyanin bioassay was modified for the rapid and convenient determination of cytokinin activity. Meanwhile, the procedures for quantitative analyses of cytokinins by HPLC and ELISA were estabolished, and these gurantee the analyses of cytokinin levels with high precision.

在细胞分裂素的活性测定中,通过改进尾穗苋苋红素合成法建立了一种简便、准确的生物试法,同时还建立了根据物理化学和免疫学原理而测定细胞分裂素的HPLC和ELISA方法,使得细胞分裂素的定量更加准确。

Our observations with transgenic plants showed that the PF40 gene affected the growth of lateral shoots developing in the basipetal pattern early after floral transition.

测量结果表明PF40基因过量表达的转基因谷子生长素的含量降低为对照的0.6~0.8倍,而细胞分裂素的含量较对照约升高0.2~0.35倍,生长素与细胞分裂素的比值在转基因植物中改变明显,降低到对照的一半。

We suppose that the changes ofcytokinin sorts are results of catabolic enzyme.

我们推测细胞分裂素的这种变化可能是细胞分裂素代谢酶活性变化的结果。

Arabidopsis response regulators,ARR4 and ARR5, were induced by the increased cytokinin contents at different time after seed germination. ARR4 responded to cytokinins at early time of seed germination, especially on the 6 d when seedlings were around true leaf initiation, while cytokinins induced ARR5 activation after 6 d of seed cultivation in light conditions, an obvious increase was on the 20 d when seedlings were around inflorescent shoot initiation.

拟南芥植物细胞分裂素反应调节基因ARR4和ARR5在植物发育的不同时期应答植物体内增加的植物细胞分裂素,ARR4的应答反应比ARR5早,种子萌发后的第6天幼苗真叶形成初期,ARR4基因被明显诱导;而ARR5的应答反应在幼苗真叶形成后的几个时间段均能检测到,并且在种子萌发后的第20天,花枝形成开始时特别明显。

Low temperature increased IAA content,but decreased the ratio of flower to apical point,ZR was similar to IAA.The change of GA was complicate,ABA content increased;Parthenocarpical cultivar had a higher ratio of flower to apical point which more than one atABA and ZR,the ratio was the highest among cultivars at GA and IAA.

低温增加了IAA含量,但降低了花器官与生长点的比值,细胞分裂素含量变化表现与IAA相似,赤霉素含量变化复杂,ABA含量增加;单性结实品种其ABA、细胞分裂素含量在低温下具有较高的花器官和生长点比值且大于l,赤霉素和生长素含量该比值也高于其他品种。

It is zeatin that mainly determines root activity and sustainleaf physiological function. However, not every kind of cytokinins hasthe capacity to promote or keep physiological function.

决定根系活力和维持叶片生理功能的细胞分裂素是玉米素,但是,不是每一种细胞分裂素都有促进或保持生理活性的作用,部分种类的细胞分裂素很可能与器官的衰老有关。

It was demonstrated that DPU itself acted as a kind of cytokinin in delaying senescence. However, it also postponed the decline of endogenous cytokinins of half-fronds.

实验结果表明,二苯脲本身具有细胞分裂素的活性,能代替内源细胞分裂素起到抗衰老的作用;二苯脲同时也能推迟半叶状体中内源细胞分裂素含量的降低。

Cytokinin KT and 6-BA were evaluated for their function in callus induction and plant regeneration. The results showed that cytokinins improved the regeneration capacity of maize and 6-BA was more effective than KT.

研究了细胞分裂素在玉米愈伤组织诱导和植株再生中的作用,结果表明低浓度(0.2mg/L)的细胞分裂素能促进玉米幼胚诱导的愈伤组织再生,6-BA的效果比KT更好。

It is zeatin that mainly determines root activity and sustainleaf physiological function.However,not every kind of cytokinins hasthe capacity to promote or keep physiological function.Some kinds ofcytokinins are related to senescence.

决定根系活力和维持叶片生理功能的细胞分裂素是玉米素,但是,不是每一种细胞分裂素都有促进或保持生理活性的作用,部分种类的细胞分裂素很可能与器官的衰老有关。

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