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分裂生长

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In this paper, we studied seasonal variation of endogenous IAA、 GA_3、ZR and ABA content of Leymus chinensis、 Agropyron michno、 Stipa grandis、 Cleistogenes sguarrosa、 Koeleria cristata、 Carex korshinskyi、 Allium bidentatum、 Artemisia frigida, Kochia prostrate and Potentilla acaulis in different restoring succession stages, which were determined by ELISA in the growing season in 2005. At the same time we recorded the phenological development and growing regulation of each kind of plant.

本文于2005年生长季,对处于不同恢复演替阶段的羊草、米氏冰草、大针茅、糙隐子草、草、黄囊苔草、双齿葱、冷蒿、木地肤和星毛委陵菜十种植物,运用酶联免疫吸附法,测定内源生长素、赤霉素(GA_3)、细胞分裂素和脱落酸含量的季节动态,同时记录每种植物的物候期和生长规律情况。

The ratio of monthly multiplications was 3-6, the vitrification phenomena of clump shoots was seldom happened, and efficiency seedlings were easily rooted besease of the optimal length between two bud burls. 3. Clump shoots subculture: On the ground of the basic media: amended MS+ BA0.2mg L-1+NAA0.1 mg L-1+saccharose3% 11 experiment was tested:(1)add to AC 0.01%, 0.03%, 0.06%, 0.1% respectively, at the same time, improving the concentration of BA to 0.5mg L-1 and NAA to 0.2 mg L-1 (2) add to VH 1mg L-1, 2 mg L-1 3 mg L-1 respectively;(3) amended MS (organic compoung and Fe2+ 1.5 double), add to saccharose 3%, 4% 5% respectively.

壮苗和有效芽诱导:以改良MS+BA0.2mg·L~(-1)+NAA0.1mg·L~(-1)+3%蔗糖+0.55%卡拉胶为基础和对照,①分别加入0.01%、0.03%、0.06%、0.1%的活性炭,同时提高BA的浓度为0.5-1mg·L~(-1),IAA浓为0.1-0.5mg·L~(-1);②分别加入1mg·L~(-1)、2mg·L~(-1)、5mg·L~(-1)的生物素,③改良MS(铁盐、有机物1.5倍),蔗糖分别增加为4%和5%,共11个处理,结果表明:活性炭对邓恩桉组培苗的生长起抑制作用,生物素所起的作用不明显,邓恩桉组培继代苗的生长离不开细胞分裂素和生长素,一旦缺少继代苗很快枯萎死亡。

Also unlike auxin, cytokinins promote the growth of lateral buds, not shoot tips.

同样不同于生长激素,细胞分裂素促进侧芽的生长,而不是顶芽的生长。

PART ONE CONSTRUCTION OF VECTOR CARRYING HUMAN INSULIN-LIKE GROWTH FACTOR-1 GENE AND THE GENE EXPRESSION IN EUCARYOTE Insulin-like growth factor-1(IGF-1) is one of the most important regulate factors which can stimulate cartilage cell to differentiate and synthesis collagen typeⅡand proteoglycan to maintain cartilage phaenotype.

第一部分:携带hIGF-1基因重组载体的构建及在真核细胞的表达胰岛素样生长因子-1(IGF-1)是在软骨发育和软骨自稳态调节中最重要的生长因子之一,能刺激软骨细胞分裂增殖,并促进软骨细胞合成Ⅱ型胶原和蛋白聚糖,维持软骨细胞的表型等。

Secondary growth Plant growth derived from secondary or lateral meristems, i.e.

次生生长:由次生分生组织或侧生分生组织的分裂活动所引起的植物生长。

Fibroblast growth factor is a kind of the polypeptide growth factors, which is known to be a mitogen for a wide variety of mesoderm-and neuroectoderm-derived cells and a neurotrophic factor for several neuronal cell types.

成纤维细胞生长因子是一种对中胚层及神经外胚层源的细胞都有促分裂活性的多肽生长因子,也是一种重要的神经营养因子。

At the same time, the process of the wound healing was observed histologically, and the regeneration cycle of epidermal cells and the temporal change in inflammatory cells were measured. Results Inflammatory cells infiltrated into wound surface were neutrophils, followed by macrophages and lastly lymphocytes. Epidermal cells proliferated most actively on PBD 3 and the mitoses of them increased significantly on day 7 after burn. The gene expression of PDGF, PDGFR and EGFR reached peaks on PBD 1 and the gene expression of EGF and TGFβ-R2 were highest on PBD 3. In addition, the gene expression of TGFβ-R1 and TGFβ1 increased significantly on PBDs 5 and 7 respectively.

结果 炎性细胞到达创面依次为中性粒细胞、巨噬细胞和淋巴细胞;表皮细胞周期变化为伤后3天细胞增殖活跃,伤后7天细胞分裂明显增多;伤后1,3天PDGF、PDGFR(血小板源性生长因子受体)和EGFR基因表达明显增强,伤后3,5天EGF、TGFβ-R2基因表达增强,伤后5,7天TGFβ-R1(转化生长因子β受体1)基因表达增强,伤后7,10天TGFβ1基因表达增强。

The morphology and structure of reconstructed tissue was detected by microscope and scanning electron microscope.Results:(1) Compared with the control group, the cellular proportion of laminin group increased in 62 ~M phase, and decreased in Go~Gi phase significantly. As shown by the microscope, the cells of control group were in low density. The cells in mass connected tightly. The microfilament appeared reticular formation. The nucleus were the same in size. The cells of laminin group were in high density and put out so many lamellipodia, filopodia, which connected with the surrounding cells. The microfilament increased, elongated, and changed from reticulodromous to sarciniform, which reached to the pseudopods. The nucleus were different in size .(2) As shown by the inverted microscope and the cell growth curve, comparing with the controlgroup, cells of each test group increased evidently. The cellular proportion of each test group increased in S phase and G2 ~M phase, and decreased in Go~Gi phase significantly, but there was no considerable interations between LN and EGF;(3) As shown by the morphological observations, the cultured cat corneal endothelial cells formed an integrated membrane, and attached to the Descemets membrane closely, which was similar to the natural tissue. The cells connected tightly to each other, and some of them arranged in hexagon approximately.

结果:(1)层粘连蛋白组处于G_2~M期细胞比例较对照组显著提高,Go~G_1期细胞比例显著下降,提示层粘连蛋白促进内皮细胞DNA合成,及细胞分裂增殖;光镜下,对照组细胞分布成团状,细胞密度较低,细胞间连接紧密,细胞内微丝结成网状,细胞核大小一致;与对照组相比,层粘连蛋白组细胞生长旺盛,细胞密度高,向周边伸出大量板状及丝状伪足,细胞内微丝增多、拉长、集结成束,伸入伪足中,细胞核形状大小不一致;(2)倒置显微镜观察及细胞生长曲线显示,各组细胞数目随时间增加而明显增多,各实验组较对照组增生显著,EGF和LN联合应用组各时间点细胞数目最高;实验组处于S期和G_2~M期细胞数目增加,Go~G_1期细胞数目减少;提示EGF、LN单独及联合应用均可促进细胞增殖,但尚不能认为二者有交互作用;(3)倒置显微镜下,组织培养的猫角膜内皮细胞排列成密集的单层,细胞间连接紧密;组织学观察发现,培养的猫角膜内皮细胞形成完整的内皮层,贴附于脱水基质的后弹力膜上,与正常的角膜内皮组织结构相似;扫描电镜下,组织培养的猫角膜内皮细胞间紧密镶嵌排列,可见某些细胞呈近似六边形排列,细胞大小不甚一致,胞核清晰。

The formation of the plant body.

内容包括:细胞分裂,极性与细胞生长,种子发育与发芽,植物体的形成,影响植物生长发育的因子,植物运动,老化与衰化,脱落,休眠。

Applying B to the 2nd node of B deficient pea plants increased the IAA concentration and export from the shoot apex, and resulted in the establishment of apical dominance.

与此相比,对缺硼的豌豆植株第2节处供硼和CPPU后明显促进了侧芽的生长,说明细胞分裂素对侧芽生长的促进作用只有在供硼的条件下才能表现出来。

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