分裂生长

BACKGROUND: Basic fibroblast growth factor belongs to active peptide, which is an effective mitogenic factor.

摘要背景：碱性成纤维细胞生长因子属于活性单腱多肽，是一种有效的促有丝分裂因子。

METHODS:NIH3T3 cell line was used.Cell proliferation with MTT method was used to study the mitogenic activity.

目的：通过对人类重组型和野生型酸性成纤维细胞生长因子（raFGF，waFGF）的比较研究，探讨raFGF与促分裂性有关的生物学作用。

Abnormal mitoses were observed in embryonic cells grown in tissue cultures in media containing PVP.

在包含PVP的血管中层的组织培养中，不正常的有丝分裂在胚胎肝细胞生长中能被观察到。

The materials required for spindle formation are formed in G2, when there is a further burst of growth before prophases begin.

在 G2期纺锤体的形成需要一些原料，在细胞分裂早期开始之前细胞还有进一步的生长。

In the present study, we collected cumulus cells oocyte complex from ovaries of two different strain mice. The cumulusenclosed oocytes were cultured for 6 h in MEM supplemented with growth factor and FSH. The meiotic maturation of these oocytes has progressed to pro-metaphse Ⅰ stage and the condensed chromosomes are visible under DIC microscope, metaphase Ⅰ spindle even can be detected under Polscope. The metaphase Ⅰ spindles of oocytes were exchanged under such microscopes. After electric stimuli, 91. 6% and 91. 6% karyoplasts-cytoplasm pairs were fused respectively. The resulting oocytes were cultured further in MEM and over 80% of oocytes released the first polar body. 79% and 77% of oocytes formed two pronuclei after in vitro fertilization and the embryos were cultured in KSOM supplemented with amino acids. Over 60% of embryos developed to blastocyst stage.

在本研究中我们在取得两种不同品系小鼠的卵丘卵母细胞复合体后，先将卵丘卵母细胞复合体置于含有多种生长因子和激素的MEM培养液中培养6小时，此时卵母细胞已进入第一次减数分裂的前中期，并且在DIC倒置显微镜下可以看到浓缩的染色体，用Polscope可以发现明显的纺锤体，借助这种显微镜通过显微操作将两种不同品系小鼠来源的卵母细胞的MI纺锤体进行互换，经过三次直流电脉冲作用后，分别有91.6％的胞质—MI核质体对融合，经过进一步的培养后，超过80％的重组卵母细胞排出第一极体，体外受精后分别有79％和77％的重组卵形成双原核，受精后的胚胎在KSOM胚胎培养液中体外培养4天后，超过60％的胚胎发育至囊胚。

Compared with SVM sorter, k-means has its superiority in rate and SVM has superiority in precision. For segmentation, region-growing algorithm which combined merge and fission was applied to segment the images of insect pest and wheat leaves.

比较得出，SVM分类器和k-均值聚类算法在处理精度和速度上各有优势；在分割上，运用合并和分裂相结合的区域生长算法分割害虫和叶片，进行自动识别。

It proves that tree top leaves suffer from water stress and hydraulic limitation becomes greater with tree height.Water stress influence tree height growth in several aspects:firstly is the influence of exterior water condition,and community average tree height is positive with exterior water condition.Secondly, hydraulic limitation influences individual tree structure and function.Xeromorphic structure,Lower water potential and turgor pressure limit cell division and expansion,which restrict gas exchange and carbon assimilation capability.However,respiration consumption increases with the spread of tree crown.Shortage of nutrient limits carbon investigation on new leave growth and may ultimately limit tree growth.Thus,the main limitation of tree height is water.

水分对树高生长的限制首先是外在的水分状况，群落的总体高度随外界水分状况的变差而变矮；再就是水力限制对单株树木的结构和功能的影响，水势降低、膨压减小使细胞的分裂和扩展受限，旱生型结构加剧水力对气体交换能力的限制、使光合碳同化能力受到限制、而呼吸消耗却随着树木个体的增大而增大，制造营养物质不足、使得营养亏缺、造成投入新枝叶的营养受限，最终限制了树高继续增长。

A study was carried out to investigate effects of cadmium stress on growth of rice seedlings, mitosis and chromosomal aberration of root tip cells.

研究了镉对水稻幼苗生长、根尖细胞有丝分裂及染色体畸变的影响。

Results show that root elongation and the mitotic index of the root tip cells were significantly decreased with the increasing Cd concentration and treatment duration.

结果表明，随着Cd浓度增加和处理时间延长，水稻幼苗根的生长及根尖细胞有丝分裂指数明显降低。

Result: With a high cancer-inhibiting rate on the animal model having been transplanted the cancer, this medicine can remarkably restrain the growth of cancer cells cultivated in vitro and improve the life quality of mouse model, and also shows obvious promotion and enhancement function on the DTH ear-swelling of mouse with cancer, the proliferation of mouse spleen lymphocyte induced by ConA and the cytokine (TNF-α、IFN-γ) of mouse with cancer respectively. It can obviously decrease the adverse effects like weight reduction, damage of viscera and degradation of life quality caused by the chemotherapeutic medicines, and has a remarkable stimulation on the lymphocyte proliferation induced by ConA. It is also able to improve the ratio of SMMC-7721 cells in G0-G1 period and reduce the cell amount in G2/M period to cut down the possibility of stepping into the division period; additionally, it can obviously inhibit some cancer genes of the cancer cells and meanwhile up regulate the cancer-inhibiting gene.

结果：本品能明显抑制体外培养肿瘤细胞的生长，对在体肿瘤移植模型动物具有较高的抑瘤率，能明显提高模型小鼠的生存质量；对荷瘤小鼠DTH耳肿胀、ConA诱导的小鼠脾淋巴细胞增殖、荷瘤小鼠细胞因子（TNF-α、IFN-γ）呈现明显的促进或提高作用；能显著减少化疗药所引起的小鼠体重降低、脏器损害以及生存质量下降等不良作用；本品含药血清对ConA诱导的淋巴细胞增殖具有明显的促进作用；能提高SMMC-7721细胞G0-G1期比例，降低G2/M期细胞数目，而减少其进入分裂期；对肿瘤细胞的多个癌基因具有明显的抑制作用，同时上调抑癌基因等。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。