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We also discovered that selenium can decrease hematolysis and effects function of vacuole toxin of HP. morphological conversion from spiralto coccoid forms occurredwhen HP was caltured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E. coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E. coli, but can not influence activity and gene equence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

结果:体外实验结果表明:一定浓度的硒作用HP后,HP菌落数量随着硒浓度的增加而减少,菌落颜色由灰白色变为砖红色,溶血现象随硒浓度的增加和作用时间的延长而逐渐消失,HP的形态由弯曲形变为球形或椭圆形,鞭毛及菌毛出现脱落现象;硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显;一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素,颜色随硒浓度增加而加深;一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长,减少重组VacA表达量,但不影响其空泡毒活性及编码基因序列;硒能增强重组VacA对胃癌细胞的抑制作用,使细胞分裂指数和集落形成率降低、S期细胞数量减少,G1期细胞总数增加;纯化后的VacA重组蛋白可被VacA抗体识别,具有良好的抗原性。

Under the light microscope, we found that transfected SGC7901 gastric cancer cells with NHE1 antisense gene were larger with abundant cytoplasm and comparatively same size of nuclei, and the ratio of nuclei to cytoplasm, mitotic count and heteromorphism decreased when they were compared with their parent cells.

与亲本细胞相比,光镜下NHE1反义基因转染的SGC7901胃癌细胞胞体增大,胞质丰富,核大小相对一致,核质比和核分裂象减少,异型性减少。

The results suggest that there might be a common biological cause underlying the atypical cerebral lateralization and schizotypal personality features.

本研究建议可能有共同的生物导因潜在於非典型大脑功能半侧化和精神分裂病性人格之间。

The first division of protoplast occurred within 3 to 5 days in modified MS liquid media (1/10 strength macroelement) supplemented with 2 mg/L NAA+0.5 mg/L 6-BA and several organic compounds.

培养在1/10 MS附加2 mg/L NAA+0.5 mg/L 6-BA和多种有机成分培养基上的铁皮石斛无菌苗叶片原生质体,3~5 d后开始第一次分裂,30d后长成胚性细胞团。

Meiosis is a special form of cell division that takes place in the reproductive organs that produce sex cells.

Meiosis是的细胞分裂的一种特殊格式在生殖器发生导致性细胞。

A core needle biopsy misdiagnosed PASH in 13 cases out of 28 cases and vacuum-assisted biopsy correctly identified PASH in all 3 cases.

4细胞性纤维腺瘤和囊肉瘤:PASH缺乏真正的间叶细胞过生长的现象,其细胞缺乏多形性,无核分裂象,也没有纤维腺瘤或囊肉瘤的上皮成分的生长类型。

Studies also indicated that genistein exsists not only in soybeans but also in many kinds of plants such as vegetables and fruits.As a potential anticancer agent, the actional mechanisms of genistein mainly includes as follows:First, genistein can depress the activity of protein tyrosine kinase and transduction pathways for the phosphorylation of receptors and mitosis signal. So genistein can lead to cells' proliferation depressed. Second, genistein has minimal effects of phytoestrogens. It can be combined with estrogen receptor and improve the synthesis of cellular sex hormone binding glulobin, and improve the activity of UDP-glucuronyl transferase. Through these pathways, it can inhibit the cell activity of breast cancer and prostate cancer.

作为一个很有潜力的抗肿瘤物质,三羟异黄酮的作用机制主要包括:①抑制蛋白酪氨酸激酶活性,可阻抑PTK引起的受体磷酸化和有丝分裂的信号传递,导致癌细胞增殖受抑;②弱雌激素作用:可通过与体内雌激素受体结合,并可增加细胞内性激素结合球蛋白的合成,增加UDP-葡糖醛酸转移酶的活性等途径抑制乳腺癌和前列腺癌细胞活性;③拓扑酶Ⅰ和Ⅱ抑制剂,抑制细胞活性;④上调细胞周期性负性调节因子P21WAF1/CIP1的表达,使之负性调节因子作用增强;⑤可阻止胰岛素样生长因子-Ⅰ、肝细胞生长因子和神经生长因子的作用而抑制肿瘤生长;⑥其他:抗氧化作用、抑制热休克蛋白、诱导细胞凋亡、抑制新生血管生成和抑制多种耐药相关蛋白等。

The reseach on activity changes of SOD, POD and CAT during the somatic embryogenesis of Y35 showed:(1) The activty of SOD was from 52.98 to 133.20 U·g-1·h-1, and remained a rising trend after early single embryo forming, this revealed that SOD might be positively correlated to the differentiation of embryogenic cell and the development of somatic embryo.(2) The activty of POD was from 0.05 to 0.50 U·mg-1·min-1, ascended firstly and desceded later, and was highest in embryogenic callus and lowest in late single embryo , this revealed that POD might be positively correlated to the division and differentiation of proembryo mass, while negatively correlated to the development of PEMⅢto late single embryo.(3) The activty of CAT was from 0.86 to 2.81 U·mg-1·min-1, showed an up-down-up trend, reaching to the highest peak at the time of early embryo formating and decreasing to the lowest at the time of early cotyledonary embryo formating, this revealed that CAT might be positively correlated to the development of early single embryo, while negatively correlated to the formation of middle single embryo and early cotyledonary embryo.The changes in activty of SOD, POD and CAT indicated these three antioxidant enzymes coregulated the differentiation and development of embryogenic cells during Larix somatic embryogenesis.4. Differentially expressed cDNA libraries of the stages of proembryo mass and somatic embryo maturation were successfully constructed by suppression subtractive hybridization.

对Y35体细胞胚胎发生过程中抗氧化酶活性变化的研究显示:(1)SOD活性在52.98~133.20 U·g-1·h-1之间,并在早期单胚形成后一直保持上升的趋势,表明其与胚性细胞的分化及体细胞胚的发育均具呈正相关;(2)POD活性在0.05~0.50 U·mg-1·min-1之间,呈现出先下降后升高的趋势,在胚性愈伤组织中最高,而在后期单胚形成时降至最低,表明其与原胚团的分裂和分化呈正相关,但与PEMⅢ向后期单胚的发育呈负相关;(3)CAT活性在0.86~2.81 U·mg-1·min-1之间,表现出升-降-升的变化趋势,在早期单胚形成时升至最高,在早期子叶胚形成时降至最低,表明其与早期单胚的发育呈正相关,而与中期单胚和早期子叶胚的发育呈负相关。

Cognitive enhancement therapy is described as an integrated approach to the enhancement of neurocognitive and social cognitive abilities, which attempts to capitalize on a presumed neuroplasticity reserve believed to respond to enriched cognitive experiences. It is used as a recovery-phase intervention for symptomatically stable schizophrenic outpatients who are socially and cognitively disabled despite reduced risk of relapse.

认知丰富疗法被形容为一种整合丰富神经认知与社会认知能力的方法,这个方法的目的在利用一种被认为会对丰富认知经验产生反应的假设性神经可塑性;这被用於尽管有复发的可能性,但是在社会上与认知上是失能的有症状稳定精神分裂病患,作为恢复期的介入性治疗。

From the narrative of the study participant ,Cabbage, the researcher discovered that because of the conflict the work brought , her internal experience was divided into two themes:"internal division and conflict" and "sexual anxiety caused from the sex offenders treatment". However, Cabbage engaged in the adjusting course of self-admittance and self-integration hard.

从白菜的叙说中可以发现到,由於工作所带来的碰撞,其个人内在的经验可区分为两大主题:「内在自我间的分裂与冲突」以及「在性侵害工作中所引发的性焦虑」,而白菜一直不断努力地进行自我的接纳与整合的调适。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。