分级

In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2～0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%～80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0～0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法，将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合，建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺：胰酶用pH4.5醋酸缓冲溶液提取后，粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5，洗脱缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5)含0.2～0.35mol／LNaCl分两步洗脱，收集激肽释放酶的洗脱峰；DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液：0.01mol／LTris-HCl缓冲液(pH7.5，洗脱液：0.5mol／LNaCl溶液，一次洗脱，收集胰蛋白酶洗脱峰；最后，亲和层析未吸附液用30％～80％硫酸铵分级盐析处理，沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5含1mol／L(NH_4)_2SO_4，洗脱缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5)含0～0.6mol／L(NH_4)_2SO_4，分两步洗脱，收集糜蛋白酶的洗脱峰。

And then a struct model of security storage system and relative security mechanism is designed, in witch a grade-accessing control sheme and a data-encrypted key distributing arithmetic based on common module general linear congruent equation class are designed to realize that storage data is accessed safely and shared by grade.

在此基础上，详细给出了iSCSI-SAN存储系统安全机制的设计方案，重点设计了基于权限证书的访问控制机制和基于共模p的线性同余方程组的数据加密密钥分配算法，实现了存储数据的安全访问与分级共享。

It is a binary format for representation of planar geometric shapes, text labels and some other information in hierarchical form.

它是一个二进制格式为代表的平面几何图形，文本标签和其他一些信息的分级形式。

In support of De Beers, GIA diamond grading produced a set of standard system.

在德比尔斯公司，GIA钻石分级支持制作了一套标准体系。

This procedure can effectively avoid glutenin fraction from contamination with other protein fractions generated by traditional Osborne fractionation scheme.

该方法避免了谷蛋白与传统的Osborne分级法所产生的其他蛋白质组分之间交叉污染的现象，而且快速稳定。

The pacemaker in Chinese self-propelled turner industry Baoding Limin Machine manufactured goldbug self-propelled turner , fermented manure turner , mud turner , garbage disposal equipment, dejecta ferment turner , tiller, organic fertilizer equipment, mushroom feeding medium turner , cow dropping turner , roll granulating machine, classification revolving screen, chain disintegrator etc.

中国自行式翻堆机行业的领跑者-保定利民机械制造金甲虫自走式翻堆机、堆肥发酵翻堆机、污泥翻堆机、垃圾处理设备、蓄勤粪便发酵翻堆机、翻抛机、翻拌机、有机肥设备、蘑菇培养料翻堆机、牛粪翻堆机、对辊制粒机、分级回转筛、链式粉碎机等。

Each country has its own system of movie gradation and examination.

世界各国都有自己的电影审查和分级制度。

Movie gradation is the development and transformation of movie examination.

分级制是电影审查制的延续和变型。

Classified information system ; gradational security ; network security

涉密信息系统；分级保护；网络安全

Combining fuzzy clustering analysis and genetic algorithm, a gradational searching algorithm of shortest path in laser cutting is presented.

结合激光切割最短路径的计算问题，将模糊聚类分析与遗传算法相结合，提出了一种分级搜索最短路径的方法，该方法对于同类问题的解决具有借鉴作用。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。