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12 Strains possessing higher peptidase activity were isolated from Chinese traditional fermented food.

试验从中国传统发酵食品中分离出12株具有较高肽酶活力的菌株,经进一步筛选,得到了两株具有较好促进干酪成熟能力的菌株,有望作为附属发酵剂应用于切达干酪生产。

Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合,而且结合是饱和性的、可被竞争的,符合配体-受体结合的典型特征,同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性,表明水稻细胞质膜上存在nsLTP的受体;利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜,再进行结合实验,结合活性几乎不受影响;而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失,表明其受体为没有经过糖基化修饰的蛋白质;利用交联剂BS~3交联配体一受体后,再进行SDS-PAGE分离和放射自显影,结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合,可能是其受体;细胞外碱化实验表明,nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应,却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应,表明nsLTP和Cryptogein结合细胞膜上相同的位点,保护了植物细胞免受Cryptogein导致的细胞程序性死亡,并诱导系统获得性抗性的产生;体外Pull-down实验表明,nsLTP144和水稻的OsCaM1具有相互作用,暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

"These results support the idea that there is a shared system between preverbal infants and nonverbal animals for representing numbers," she said.

从我们的研究结论来看,他们很快就会以某种方式获得这一感知数字的能力,并将其从感官信息中分离出来。

Is better than that of using Na〓S merely and the profit per year is over 6, 000, 000 yuan. External potential-control flotation column and The Cu-Mo bulk concentrates flotation separation. With appropriate combination of the principle of sulphide flotation pulp electrochemistry and the technology of flotation column show the advantage over flotation equipment, External potential-control flotation column was studied successfully, laboratory potential-control flotation column was designed and its structural principle, the relationship of electrode structure and the activity of separation, the electrochemistry principle of potential-control flotation column also was investigated.

用具有较强电化学还原能力的无机物(如硫化钠、连二亚硫酸盐等)与能和硫化铜矿物表面发生选择性吸附的小分子有机化合物组合使用、成功地实现了混合精矿体系中黄铜矿的抑制、解决了德兴铜矿铜钼混合精矿浮选分离时、硫化钠用量大、生产成本高,生产指标不稳定的技术难题,研究结果表明,用组合抑制剂3.8kg/t可取代生产中硫化钠45kg/t的高用量,并获得优于单用硫化钠的好指标,年直接经济效益600万元以上。

With the isolated and purified PSP as test materials, the chemical model systems were used to produce and OH. resp. And the scavenging capability of PSP on free radicals, its inhibition effect on pyrogallol autoxidation and the effects of PSP on SOD activity and MDA content in tumor-bearing mice were studied.

方法]以已分离提纯的甘薯多糖为试验材料,利用分别产生氧自由基O2(上标-和羟自由基的化学模型体系,研究甘薯多糖清除舟自由基能力及其对邻苯三酚自氧化的抑制作用和甘薯多糖对荷瘤小鼠SOD活性和丙二醛含量的影响。

Quadrature couplers are an excellent way for power distribution due to a high isolation and high power capability.

正交耦合器是一个很好的方式,具有配电,高分离和高功率的能力。

Separation of enantiomer for the acidic racemate could be improved with a lower pH value of the mobile phase since the dissociation of acidic compound was impressed, but it did not affect significantly the resolution of neutral compounds.

在流动相中使用低pH值,能有效抑制酸性化合物的解离,从而显著增强其手性识别能力;对于中性化合物,流动相中pH对手性分离影响不大。

Isolated cells are MSCs, with the property of stem cells. The third and fifth passage cells are pure, with strong reproductive capability.

分离培养的细胞为间充质干细胞且成分单一,具有干细胞特性,第3代和第5代细胞很纯且其增殖能力强。

A yeast strain was isolated from sesame flower and classified as Candida tropicalis, with phenol, resorcin and bengidine as the sole carbon and energy resource.

实验室分离的一株热带假丝酵母(编号为8953)具有较强的苯酚降解能力,并可以利用苯酚、间苯二酚、联苯胺等芳烃为唯一碳源和能源生长。

Majority among 39 strains can renodulated on the hosts and on Robinia pseudoacacia ___a legume.

项目分离获得的39株菌株中,多数菌株能回接宿主结瘤,多数回接结瘤的菌株在剌槐的根上也表现出侵染致瘤能力。

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