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After 4 passages in culture, the MSCs were implanted into 96 foveola with cell density of 0.08,0.1,0.2,0.4,0.6,0.8,1,2,4,6,8,10(x104/ml) respectively. Meanwhile, the MSCs were cultured in Dulbecco's modified Eagle's medium culture medium with different concentration of fetal bovine serum, the 3-4,5dimethylthiazol-2-yl-2,5-diphenyl tetrazelium bromide assay were used to determine the proliferation index of MSCs 72 hours later, the cell cycle were determine by cytometer also.

贴壁筛选法分离、纯化MSCs,取4代以后细胞,以不同密度接种于96孔培养板内,分别以不同浓度血清进行培养,72h后,采用四甲基偶氮唑盐比色法测定MSCs增殖能力并用流式细胞仪测定细胞周期。

The authors have separated and purified four strains that can b e used to decompose the cellulose and starch at a great rate from the zymotic wa ste,starch factory's sludge and putrid haulm.

从发酵的垃圾、淀粉厂污泥、腐烂桔杆等样品中,分离、纯化、诱变、筛选出分解纤维素、淀粉能力较强的 4种菌株,按不同比例混合制备出有机固体废物发酵剂。

In this study, 12 strains of alginic acid decomposing bacteria in surface of Laminaria japonica were isolated using microbiological methods. Five strains of them exhibited highly infective activity.

本文利用微生物学的方法对病烂海带表面的褐藻酸降解菌进行了分离和筛选,得到五株强降解能力的菌株。

The results of hot test show that Am can be well separated from fission product lanthanides by HBTMPDTP extraction.

HBTMPDTP具有较好的辐照稳定性,吸收剂量<104Gy时,HBTMPDTP萃取分离Am与镧系元素的能力基本不变。

Through morphological study, biophysical and biochemical tests ,we discovered that this strain was a kind of osmophilic yeast and can fermented glucose,sucrose and maltase.

分离筛选到了一株产赤藓糖醇能力高的菌种B84 5 ,通过对B84 5的形态、生理生化特性试验,发现该菌耐高渗,能发酵葡萄糖、蔗糖、麦芽糖,细胞为圆形、椭圆形,单边或多边芽殖,有真菌丝,在固体培养基上培养 7d后颜色开始变深,由黄色变成褐黑色,参阅真菌鉴定手册,初步鉴定为圆酵母属菌 Torulasp。

PartⅠTheoretical Research MSCs is stem cells capable of multi-directional differentiation arising from mesoblast, which principally lies in stroma of connective tissues and organs, especially abundant in marrow, even divorted from fetal umbilic blood, differentiates into osteocytes, chondrocytes, tendon cells, myoctes, nerve cells, adipose cells and hematopoiesis stromal cells and so on.

理论研究间充质干细胞(mesenchymal stem cells,MSCs)是中胚层来源的具有多向分化能力的干细胞,主要存在于全身结缔组织和器官间质中,以骨髓组织中含量最为丰富,胎儿脐血中亦可分离得到。

In chapter five, effect of temperature on the chiral separation of the molecular imprinting polymer was investigated and it was found that the chiral separation process was enthalpy-controlled.

第五章考察了温度对分子烙印聚合物的手性拆分能力的影响,进一步加深了对分子烙印手性固定相分离对映体的作用机理的认识。

Objective To explore the culture method of rat embryonic neural stem cells and the ability of neurogenesis after being cultured long in vitro.

目的 探讨神经干细胞培养的分离传代技术及其在长期培养条件下的神经发生能力。

We would like to make our students to know and master the basic way of the chemistry experiments, the use of the normal apparatus of inorganic and analytical chemistry, the character of the normal inorganic compounds and

课程的目标是使学生了解和掌握化学实验的基本方法和常见的无机与分析化学仪器的使用,了解和掌握一般无机化合物的性质以及分离提纯的方法;熟悉常见的无机化合物的制备方法,掌握测定有关重要化学常数的方法和原理;熟悉滴定分析的基本操作,掌握定量测定无机化合物的主要化学滴定的方法和原理,从而培养学生能用化学观点与方法来观察分析和解决实际问题的能力。

In this study, cow manure was collected from the pasturage of National Chung Hsing University.

本研究从中兴大学牧场采集乳牛粪便,尝试从中分离具有强木质纤维素分解能力的厌氧真菌。

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