分离能力

Experimental transmission of an unnamed bovine Babesia by Hyalomma spp., Haemaphysalis longicornis and Boophilus microplus: Experiments were undertaken to determine the mode of transmission to cattle of an unnamed Babesia sp.

二。采用蜱传播试验，研究了璃眼蜱蜱种、长角血蜱和微小牛蜱对牛的巴贝斯虫未定种的传播能力与传播方式：为了确定小亚璃眼蜱、残缘璃眼蜱、麻点璃眼蜱、长巴贝斯虫未定种的发现及牛巴贝斯虫病的免疫诊断和药物防治研究角血蟀和微小牛蟀对在我国分离的牛的一种未命名的巴贝斯虫的传播能力与传播方式而进行了研究。

We get to the conclusion that the function set of DCRBFN is dense in a varible-decomposable function set. The result shows that DCRBFN has excellent approximation ability.

DCRBFN系统的逼近能力分析，我们得到DCRBFN的函数集在变量可分离函数集中稠密的结果，因此DCRBFN具有良好的逼近能力。

The recognition capability and adsorption rate of MIP can be significantly enhanced by selective modification.Separation factor could be increased from 1.60 to 3.06 after modification when naringenin was chosen as competitive molecules, and the time needed for adsorption equilibrium could be reduced from 100 min to 60 min.Within a certain range, increasing template molecule concentration or diazomethane dosage, or adopting solvent which had weaker hydrogen bond action with template molecules could all improve the modification effect.

结果表明，选择性修饰能明显提高MIP的识别能力与吸附速度，以柚皮素为竞争分子，分离因子可从修饰前的1.60提高到修饰后的3.06，吸附平衡时间从修饰前的100 min降低至修饰后的60 min；在一定范围内提高模板分子浓度，增大重氮甲烷用量，采用形成氢键能力较弱的溶剂，均能提高修饰效果。

The most disturbing of the Geomancer's power, however, is his ability to separate his being into multiple selves, each as powerful as the original and making him potentially four times the trouble for the unlucky who encounter him.

然而土占师最扰人的能力是他能将自身的存在分离产生多个另体，每个另体的能力都与本体不相上下，让他成为惹上他的倒楣鬼的四倍大的大麻烦。

The research of the characteristics of biology and the osteogenic and adopocitic induction are comparatively mature, showing many biological characteristics such as adherence, plasticity, heterology, self-renewal, rapid clone formation and transplantation. Adherence combined with density gradient centrifugation method is widely used in the isolation and purification of BMSCs.

骨髓间充质干细胞现阶段研究方向主要包括生物学特性、诱导分化和调控机制等内容，对其生物学特性和成骨成脂诱导方面研究比较成熟，在培养分化过程中展示出贴壁性、可塑性、异质性、自我更新能力、快速形成克隆能力、可移植性等生物学特征，贴壁法联合密度梯度离心法在骨髓间充质干细胞的分离、纯化过程中被广泛采用。

Bacterial strains were isolated from humus mud by employing the dilution methods. Seven cellulose-degradation Strains marked as B-37, B-35,B-31, B-25, B-17, Z-a, and Z-b were selected according to the hyaloid rings on the medium of carboxymethylcellulose sodium Congo red and their efficacy of decomposing filter paper. Z-b had the strongest ability in decomposing filter paper among the seven strains and 16S rDNA analyzed indecated that it was Stenotroplaomnonas maltophilia owning to 99.8% homology. Comparing clony size of the seven strains in media with different carbohydrates and inorganic salt, we found that the potato extract and inorganic salt which contained CaCl2 0.20, MgSO4 1.25, NaCl5.00,(NH4) SO4 1.30, KH2 PO4 1.35, FeSO47H2O 0.015, Na-EDTA 0.02 were suitable for the bacteria growth.

用稀释法从腐殖泥中分离菌株，根据它们在梭甲基纤维素钠-刚果红培养基上的透明圈直径及对滤纸的崩解能力，获得7株纤维素分解细菌（编号：B-37、B-35、B-31、B-25、B-17、Z-a和Z-b），其中菌株Z-b的纤维素崩解能力最强，分子鉴定结果表明它与Stenotrophomonas maltophilia的16S rDNA序列有99.8%的同源性，初步确定为嗜麦芽窄食单胞菌。

Our cell line belongs to neural precursor and is multipotent,which has the ability to undergo self-renew.So,they are the stem cells of the central nervous system.

我们分离的细胞具有自我更新能力和多分化潜能，属于胚胎早期细胞，有很强的增殖能力，是中枢神经系统的干细胞。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

Activity of alkaline phosphatase and ability of formation for calcifying nodules were determined.

抽取健康成人骨髓组织，用Percoll分离液分离出骨髓中的单个核细胞，在含体积分数为10%小牛血清的高糖DMEM培养液中，置于37℃、含体积分数为5%的CO2湿化空气孵箱中培养，通过传代培养扩增骨髓基质干细胞，传三代时改用含地塞米松、β-甘油磷酸和维生素C的条件培养基培养，用倒置显微镜、HE染色观察增殖和分化情况，并测定碱性磷酸酶活性和钙结节形成能力。

This thesis put that, although ETF and BATF had the same biogas yield, their hydrogen yields were remarkably different in the same testing condition because the hydrogen content in their produced biogases were different due to the difference of their metabolic pathways.

本研究先后从连续流反应系统的乙醇型发酵阶段和丁酸型发酵阶段分别7次采集分离样品，从中分离到215株产酸发酵细菌，其中有36株具有产氢能力。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了