分离用的

Study on glutenin subunits,GMP content and their relationships with(T.aestivum L.)With the analysis of high and low glutenin subunits of common wheat(Triticum aestivum L.)in China,the correlations between glutenin compositions,glutenin macropolymercontentand breadmaking quality were studied.Also a rapid and efficient detection method of geneticpolymorphism at Glu-D1y loci in wheat was established using polymerase chain reaction.

分析了100份小麦农家品种和241份育成品种的高、低分子量谷蛋白亚基的组成以及有关的品质性状，研究了高分子量谷蛋白亚基频率的变化，用单向一步SDS-PAGE的方法分离LMW-GS，并对我国小麦品种进行初步分类；在亚基水平上研究了不同的亚基或亚基组合对品质性状的影响；在聚合体水平上研究了谷蛋白大聚合体的含量与其他烘烤品质性状的相关性及其在面包体积预测中所起的作用；在基因水平上利用PCR技术研究了1D染色体上控制y-型谷蛋白亚基的Glu-Dly位点基因的多态性。

FT-IRDSC tests were taken to analyze the structure,and the terms-mechanical properties,shape recovery behaviors and tensile properties were discussed to study the extender influence on SMPU.The results show that SMPU extended by ethanediol and FA has good micro-phase separation structure and soft...

结果发现，扩链剂对SMPU有一定影响，用FA、ED扩链的SMPU具有较好的微相分离，而DE扩链的具有较好的软段结晶性能；FA、HD的SMPU具有较低的形状回复温度和较快的形状回复速率，HD、DE扩链的则具有较好形状固定性能，FA、DE扩链的SMPU循环使用性能较好；同时FA、HD的SMPU具有较高的弹性模量与力学强度。

Based on this idea, the author has chosen two typical loess sections of Luochuan, and Xifeng for this study, collected the successive loess columns; To probe the conditional experiments of BeO sample preparation and measurement, and improve the existing method; On the basis of the technology improvement, the author has finished 402 samples' BeO preparation and AMS measurement; Also at the same time, to separate quartz from samples at 1 cm interval for both Luochuan and Xifeng sections systematically, to carry out measurements of quartz grain-size, SUS, grey scale, carbonate content and bulk sample density; By using the grain size model, the author has set up the chronological framework for both Luochuan, and Xifeng sections and the time sequence of millennial scale abrupt climate events recorded in loess strata, and reconstructed the East Asian monsoon history over 130ka by using multi proxies; Finally, to discuss the possible mechanisms of palaeoclimatic changes tentatively.

详细地球科学的发展离不开高精度和高分辨率的研究。运用长寿命核素如〓Be作为环境过程的示踪，已成为不可替代的有效工具。本论文选择洛川、西峰两个典型的黄土剖面，连续采集柱状样；探索了BeO样品制备、测量的条件实验，改进了原有的方法，完成了402个BeO样品的制备和AMS测量；同时还系统地分离洛川、西峰剖面高分辨率（1cm）黄土、古土壤样品中的碎屑石英，测量了碎屑石英粒度，以及全样的磁化率、灰度、碳酸盐含量和容重等古气候代用指标；采用粒度年代模式建立了洛川剖面和西峰剖面的年代标尺，建立了黄土地层记录的千年尺度的气候突变事件的时间序列，多指标重建了最近130ka东亚季风变迁史；初步讨论了古气候变迁的可能机制。

The accelerator physics problems of the upgrading program were discussed here, which include the space charge effect on the very low energy beam line, non-parity acceleration at the injector cyclotron SFC, longitudinal match between two cyclotrons, strong magnetic field leakage afluence on the beam transfer at low and very low energy, residual first harmonic magnetic field in the main cyclotron SSC injection region, the low efficiency and beam quality of heavy ions after through stripper etc.

主要问题有：超低能束流传输的空间电荷效应的影响，扇聚焦回旋加速器SFC高频加速电压不对称对束流轨道的影响，用半频聚束的方式补偿两台回旋加速器的纵向不匹配，前束线上聚束器的工作模式的选取，强杂散磁场对超低能和低能束流传输的影响，分离扇回旋加速器SSC注入区过垫补磁场对注入束流轨道的影响，SFC和SSC的单圈引出，重离子通过剥离膜后的束流损失和束流品质的变坏等。

One pair of primers that amplified the gB gene of pseudorabies viruswas designed and synthesized.PCR technique detecting the DNA of PRV was established after selecting the best reaction conditions.This technique was applied to specifically amplify the 281 bp DNA fragment of the PRV strains including Fa,Fb,Bartha,BJ,GD,V2F4,S,S3,SR,Buk,Shope,Norden,Mink Ⅲ,HB,F8,F9 and F12 in cultured samples.The negative results were achieved from Vero cells,swine vesicular disease virus,hog cholera virus,Japanese encephalitis virus,porcine reproductive and respiratory syndrome virus,porcine parvovirus,foot and mouth disease virus F29 strain,O3I3 strain,T509 strain and O Ⅱ MF249 strain.The results of sequencing showed that the PCR method was of specificity.The sensitivity of PCR reached 15.8 pg of PRV Fa strain DNA.The tissue samples obtained during 1994 and 2000 were detected,and the results showed that the sensitivity of PCR was more sensitive than virus isolation and the Sandwich ELISA.The PCR was applied to detect 191 tissue samples from 31 pig farms obtained from Guangdong,Fujian,Hainan Provinces during 1999 and 2000,50 samples(26.2%)were positive and 22 pig farms(71%)were positive.

根据伪狂犬病病毒gB基因的序列，设计并合成了一对引物，以闽A株细胞培养毒为模板，筛选最佳反应条件，建立了检测PRV的PCR方法应用该方法对Fb、Bartha、BJ、GD、V2F4、S、S3、SR、Buk、Shope、Norden、MinkⅢ、HB、F8、F9、F12等毒株的细胞培养液进行基因扩增，均获得了分子量为 2 81bp的特异性目的DNA片段，而对Vero细胞与FMDV、SVDV、HCV、PRRSV、JEV、PPV等病毒进行检测，结果均为阴性，没有出现交叉反应对PRV毒株扩增的产物测序，结果序列与文献报道一致，证明PCR扩增产物和方法的特异性对 1994～ 2 0 0 0年期间送检的临床样品和保存的PRV毒种，用病毒分离、双抗体夹心ELISA和PCR等 3种方法进行检测，结果前 2种方法检测为阳性的，PCR检测均为阳性；PCR检测为阴性，前 2种方法检测也为阴性；可是，前 2种方法检测为阴性的，PCR却检测出部分阳性；经x2 检验，证明PCR检出率明显高于前 2种方法的检出率对PRV闽A株细胞毒提取物DNA进行检测，其最低检出量为 15 8pg 对 1999～ 2 0 0 0年期间广东、福建、海南等省的 31个大中型猪场送检的 191份病料进行检测。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。