分离用的

The improved watermelon inbred line wbl 9 with less-branching is developed by using donor parent of wbl 1 with 1 cross, 2 backcrosses with superior inbred line 015 and 6 generations of pedigree selection.

用具有弱分枝性状的wbl 1作供体亲本，用优良的西瓜自交系015作父本，通过1次杂交、2次回交和连续6代的自交分离和纯化，育成了具有弱分枝性状的优良自交系wbl 9。

In this paper, purification of growth hormone receptor from ovine liver and identification of its biochemical character have been done.Firstly, by homogenizing, triturating, high-speed centrifuging, hypervelocity centrifuging and sucrose density grade centrifuging cell membrane of ovine liver was separated and its electron microscope slice wasprotein was disL~olved with TritonX-100,amL-thebinding activity between cell membrane or membrane protein and ~I-hGH using RRAwas measured, perfect saturated curve wasgained. Thirdly, specific binding of GILR protein was measured ,by SDSPAGE, its molecular weight is about 1 19KD.

本研究采取新鲜羯羊肝脏，匀浆、研磨，经高速离心，超速离心和蔗糖密度梯度离心，分离肝脏质膜，得到肝脏质膜的电镜切片：其次，用去垢剂TritonX-100增溶膜蛋白，利用放射受体分析法，测定细胞膜及膜蛋白与~(125)I-hGH的结合活性，均得到理想的饱和曲线；再次，将两点结合试验法与凝胶层析法结合检测到特异结合物，然后，经SDS-PAGE电泳分析，纯化后的样品呈现一条带，与标准分子量比较，得出分子量为119KD。

Two of the 9 phenol degrading bacteria were isolated from activated sludge for treating the wastewater from coke industry, while the rest from soil samples.The 2 strains from a coke plant showed one major band of 1.1 kb in ERIC-PCR.

Bruijin用ERIC特异的引物以R.meliloti的染色体DNA为模板进行PCR扩增，发现供试的30多个菌株的产物在琼脂糖凝胶电泳分离时能各自显出独特的条带特征［3］。

The product line based on the current packing line adopts the design of separating the principle machine from the conveyer belt Counting, filling in the paper and screwing the lid using one conveyer belt get over the hard problem of adjusting the product and knocking over the bottles easily Counting machine and swing machine use the local advanced structure and avoid the phenomena of damaged easily and big noises To fix up an automatic weeding device on the screwing machine can abate the pollution and improve the producing efficiency largely and accord with the standard of GMP mostly.

根据目前的产品线包装线采用了分离设计原则，从输送带计数机、在充填和倒盖，用一纸输送带上摆脱了产品结构调整的问题难以在1338瓶易票机、摇摆机采用先进局部结构破坏的现象，避免大的噪音，况且费力除草器就自动倒机可以消减污染，提高生产效率，并在很大程度上符合GMP的主要标准。

Its principle is as follows:red blood cells are labeled with NHS-Biotin,participated in the circulation and ages in vivo.Using the high affinity between biotin and avidin,the labeled RBCs with specific ages are separated.

其基本原理是用生物素标记一定量的红细胞，使之参与血液循环，并在体衰老，利用biotin与抗生物素蛋白之间的高亲和力，分离带有标记的、具有特定年龄的红细胞。

Methods: The cerebral cortical astrocytes and neurons of rats were cultured and the model of simulated cerebral ischemia and reperfusion was set up in vitro. The activity, survival rate, death rate of the neurons, leakage rate of lactate dehydrogenase and the activity of nitric oxide synthase of cerebral cortical neurons were measured after 18 boors of reperfusion.

分离纯化培养大鼠大脑皮质Ast和神经元，体外模拟脑缺血再灌注损伤模型，用ACM培养损伤后的神经元，测定其神经元的活性、存活率、死亡率、培养液乳酸脱氢酶的漏出率和NOS强阳性细胞的表达量。

In order to investigate the influences of different drying methods on extraction and separation of ginsenosides, three drying processes, such as drying in the sun, drying in oven and microwave drying, were used to dry fresh ginsengs Methods: The ginsenosides of the dry ginsengs were extracted by poaching and microwave heating, and were separated by foam separation.

采用自然晾晒、烘箱乾燥和微波乾燥三种不同的方法对鲜人参进行了乾燥，观察乾燥效果；采用水煮和微波加热的方式对三种乾燥人参中的人参皂苷进行提取，进一步用泡沫分离法将各种不同皂苷成分进行分离和浓缩，测定总皂苷和单体皂苷的浓度，计算皂苷回收率。

The gas chromatography quantitative analysis conditions of gum rosin were studied by selection of chromatographic columns and chromatographic resolution conditions with the pure rosin sample of P.

用纯思茅松松香样品，通过选择色谱柱和色谱分离条件，研究确定了脂松香气相色谱定量分析的条件，定量分析了松香树脂酸的相对百分含量；定量分析数据通过统计学中的Grubb′s检验法进行误差检验，在确定的条件下脂松香气相色谱定量分析结果具有稳定性、准确性和复现性结果；对马尾松、湿地松松香定量分析也取得同样结果。

objectiveto explore a newsimple and effective surgery technique of eyebow lackness.methodstake aitem ofintegrity scalp with hair from the rest head or the back of the auris.separate the hair trunk and bursa with special appliance.

目的：寻求一种治疗眉毛缺损的简单有效的手术方法方法：在耳后或枕后发际内切取一条带毛发的完整头皮。利用特殊器械将毛干及毛囊完整分离，用植毛针把带完整毛囊的毛发移植于眉毛缺损处。

The marriage of Adgar Snow and Helen Snow not only made some necessary conditions for Adgar to create his "Red Star Over China", but also stimulated greatly the creation desire of Helen; whereas their divorce made Helen herself into her literary creation of the reportage and finished about 50 reportages, most of which were about the revolution and the construction of China.

爱德加·斯诺与海伦·斯诺的结合，为爱德加写作《红星照耀中国》创造了条件，也极大地激发了海伦的文学创作欲望；他们的分离又使海伦·斯诺全身心地投入到报告文学创作中，使她用毕生精力写出几十本有关中国革命与中国建设的报告文学，爱德加与海伦的文学创作既有相似之处，又风格各异。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。