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An astaxanthin overproducing strain was obtained by mutagenesis.In 200L common stirring fomenter, 60mg/L - 70mg/L astaxanthin were harvested by fed-batch cultivation of Phaffia rhodozyma. Results of optimization of extract condition indicated astaxanthin productivity was 1.14mg/g by acid methods under optimal condition. This achievement reached to the leadinglevel of the field in China, and also advanced method internationally. In the same time, it is a good basis for the further research for the fermentation of natural astaxanthin.

分离选育出虾青素高产的海洋红酵母,在200L中试罐中发酵虾青素,虾青素的产率达60-75mg/L发酵液;对天然虾青素的无毒提取技术进行研究,虾青素的提取得率为1.14 mg/g干菌体;虾青素发酵水平和提取技术水平为国际先进,国内领先,为用发酵法生产天然虾青素打下坚实的基础。

Bacterial strains were isolated from humus mud by employing the dilution methods. Seven cellulose-degradation Strains marked as B-37, B-35,B-31, B-25, B-17, Z-a, and Z-b were selected according to the hyaloid rings on the medium of carboxymethylcellulose sodium Congo red and their efficacy of decomposing filter paper. Z-b had the strongest ability in decomposing filter paper among the seven strains and 16S rDNA analyzed indecated that it was Stenotroplaomnonas maltophilia owning to 99.8% homology. Comparing clony size of the seven strains in media with different carbohydrates and inorganic salt, we found that the potato extract and inorganic salt which contained CaCl2 0.20, MgSO4 1.25, NaCl5.00,(NH4) SO4 1.30, KH2 PO4 1.35, FeSO47H2O 0.015, Na-EDTA 0.02 were suitable for the bacteria growth.

用稀释法从腐殖泥中分离菌株,根据它们在梭甲基纤维素钠-刚果红培养基上的透明圈直径及对滤纸的崩解能力,获得7株纤维素分解细菌(编号:B-37、B-35、B-31、B-25、B-17、Z-a和Z-b),其中菌株Z-b的纤维素崩解能力最强,分子鉴定结果表明它与Stenotrophomonas maltophilia的16S rDNA序列有99.8%的同源性,初步确定为嗜麦芽窄食单胞菌。

With the dextrose manipulation of his digits,the master clammier extricates the muculant mollusk from its libidinous mana.

那位挖蛤蛎的人用他那十分敏捷的手指头把浑身涂满粘液的软体动物从它们的结合状态中分离开来。

Intelligent answering based on answer library realizes the automatic answer of user's questions based on nature language with the Chinese segmentation algorithm based on maximum matching method.

系统的实现基于J2EE三层架构体系,在中间层采用先进的MVC设计模式思想,实现页面显示与业务逻辑的分离,有效提高系统的可维护性、可扩展性和可复用性。

Sodium borate was chosen as the buffer andα- naphthylamine and NaBH3CN as the derivation agents. The optimal conditions were 60 mM pH 11 sodium borate buffer, 1.0 psi pressure injection for 90s. Compared with the normal CZE method, the detection sensitivity of glucose was enhanced by more than 20 times, and that of fructose nearly 60 times.

本文的最后一部分,选用硼砂作为缓冲液,以α-萘胺及NaBH3CN作为衍生化试剂,探索了采用场放大进样方法,用毛细管电泳对葡萄糖和果糖进行分离和富集的实验条件,最优的实验条件为60 mM,pH 11.0硼砂缓冲液,1.0 psi压力进样90s,和常规方法相比,葡萄糖的检测灵敏度提高了20多倍,果糖提高了近60倍。

To simply thd research process, they isolate the individual objec from its surroundings, and describe its performance in thd time and space domain with linear continuous-variable epuations, arriving at a deterministic analysis, such as Newton's Second Law of Motion and the Epuation of Moving Body.

为了简化研究工作,先把研究对象孤立起来,使之从其所处环境中分离出来,再把它的性能用线性连续数学公式在其所处时空领域中进行描述,得到确定性的分析,例如牛顿的力学定律、运动的方程式等。

The resolution and renaturation efficiency of lysozyme were found to be independent of the flow rate and gradient time when the volume of mobile phase was kept a constant.

在计量置换模型的基础上首次提出了流动相用量估算公式,用实验对其进行了检验,并得出在保持流动相用量不变的条件下,改变流速和梯度时间对分离度和变性蛋白的复性效率影响不大的结论。

The theory of 0.5th order differential polarog. for multistep reversible charge transfers is proposed, and a theor. polarogram was computer simulated. The effect of both the standard potential separation DE? and the no. of electrons on polarograms is discussed.

卷积伏安法是近十年发展起来的一种新电化学分析方法,本文报导了逐级电子转移过程的0.51次微分电分析法的理论和实验结果,用计算机模拟了理论极谱图,讨论了标准分离电位和电子转移数对极谱的影响。

Firstly, milling mechanism of planetary ball mill and process of flaking microsphere aluminum powder were introduced simply in milling process,and several main factors were also discussed in course of milling aluminum powder by planetary ball mill.

首先,简单介绍了球磨工艺过程中行星式球磨机的球磨机理及微球铝粉片状化过程,并讨论了用球磨机在球磨铝粉过程中的几个影响因素;其次,是分离工艺过程中筛分和过滤待改进的工艺条件;第三,探讨了捏合过程对整个产品体系稳定性的影响;最后,简易配制涂料,采用相关仪器对产品质量进行评价,并提出三方面的改进要求。

The preparation of 235 U targets and the recoil catcher assembly,determination of neutron flux by Au flux monitor,radiochemical separation and detection of 136 Cs and 137 Cs are studied.

主要研究内容包括:235U靶的制备;反冲捕集装置的设计加工;用Au作为中子监测器监测135Cs活化时的中子注量率;137Cs和136Cs的放化分离和测量。

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书,王祥生,李德昌。安徽省首次发现嗜群血蜱。

Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。