分离用的

Pathological changes of bone and periarticular abnormalities have been recorded to account for osteomalacia, osteoporosis, spontaneous fractures, adult rickets,steoarthritis,osteochondritis dissecans, chrondo calcinosis, subchondral cyst formation, and azure lunulae of the fingernails.

骨的病理改变和关节周围的异常可以用来解释骨软化症，骨质疏松症，自发性骨折，成人佝偻病，骨性关节炎，分离性骨软骨炎，钙质沉着症，软骨下囊形成，指甲的天蓝色弧形影。

Level of IFNγ secreted by splenocytes of immunized mice up-on antigen-specific stimulation was detected by using a cyto-pathic effect reduction assay and splenocytes proliferation of immunized mice in response to antigen restimulation was measured by MTT method.

分离免疫小鼠的脾淋巴细胞，体外用抗原再刺激，生物学方法检测IFNγ产生水平和MTT法检测淋巴细胞增殖。结果 RT-PCR检测证实pTB30m可在CHO细胞中表达。pTB30m免疫小鼠可引起较高水平的Ag85B特异性的抗体应答。

In addition, a further study was made of the technique of extraction, isolation and purification of GLA. The factional extraction used in the laboratory with n-hexane and ethanol was changed into the whole extraction of 6〓 solvent, which simplified production technique and reduced cost of production. The fungal lipid extracted from the fermentation in the medium-scale test was concentrated by the urea adducting method with methanol as solvent, at 75～80℃ for 3 hours. After GLA had been adducted twice, the content of GLA came up to 84. 25%, around a twelfth as much as the original concentration.

并对γ—亚麻酸的提取和分离纯化工艺进行了研究，将实验室中用正己烷和乙醇分步抽提的工艺改为用6〓溶剂一步抽提技术，从而简化了生产工艺，降低了生产成本；采用脲素包合法，以甲醇作为溶剂，温度控制在75～80℃，包合时间为3h，对中试发酵所提取的菌油进行了浓缩，γ—亚麻酸两次包合后，GLA含量达到84.25%，比原浓度浓缩了近十二倍。

Current study compared the effects of the method of the low temperature storage without sterilization and preservatives with the method of sterilization under 80 ℃ for 15 min and then room temperature storage with potassium sobital acid on the gingers' flavor quality, color, nutrient and microorganism etc.

该试验对保存的泡姜进行了感官评价；采用分光光度计法对泡姜中的Vc、蛋白质、可溶性总糖和姜辣素进行检测分析；用高效液相色谱检测有机酸；采用稀释平板法对泡姜中的微生物进行纯化分离。

Three hundred and twenty-five isolates of Magnaporthe grisea collected from rice plants in Wujiang, Yixing, Tongzhou, Gaoyou and Ganyu of Jiangsu province from 1997~1999 were tested for mating type and fertility with standard tester strains. Seventy-four tested isolates produced perithecia after mated with tester strains, in which, 48 were MAT1-1 and 26 were MAT1-2 mating type.

用标准菌株对1997~1999年在江苏省吴江市、宜兴市、通州市、高邮市和赣榆县采集的325个稻瘟病菌单孢分离菌株的可育性和交配型进行了测定，结果表明江苏省稻瘟病菌菌株的育性较低，可交配率为22.77%，可育率仅为7.08%。

The recombinant viruses, with the gene of reference RV strain VP2, field strain T114 VP6 and T73 G1-serotype VP7, respectively, co-infected Sf9 cell at a multiplicity of infection of 0.5. Harvested the supernatants of culture when the cells were lysed fully (about 7-10 days), or harvested cells before cell lysates (about 4-5 days after infection) and then treated the cells with lysing solution. The supernatants of and cell lysates were ultracentrifuged twice with 40% sucrose cushion at 93 000g, and the sediment products were harvested with TNC buffer. The purified VLPs samples were separated by SDS-PAGE, and then stained by Coomassie blue and silver nitrate and detected by Western blot to analyze the composition of VP2, VP6 and VP7 and their specificity. The morphologic structure of recombinant VLPs was detected by EM.

含标准株VP2、地方株T114 VP6和地方株T73 G1型VP7蛋白编码基因的重组病毒以0.5 MOI共感染Sf9细胞制备2／6／7病毒样颗粒，待细胞完全裂解后收获培养基上清或感染后5天细胞裂解前收获细胞并用裂解液裂解，培养基上清和细胞裂解液样品两次40％蔗糖垫93000g超速离心，沉淀样品经SDS-PAGE胶分离，用考马氏亮兰和硝酸银染色以及Western blot检测样品中VP2、VP6和VP7蛋白的组成以及特异性，电镜检测重组病毒样颗粒的形态结构。

But when making positive pole with silicon film or grain when researcher, a large number of coming back the lithium atom that leave can destroy these silicon, make at the same time its and the metal below base piece detached, reduced the effect of batteries finally.

但是当研究人员用硅薄膜或颗粒制成阳极时，大量旋离的锂原子会摧毁这些硅，同时使其与下面的金属基片分离，最终降低了电池的功效。

If you buy the album with glue, but you also do not want to stick paper cutting die can be used will be transparent cellophane paper-cut separation of the glue.

如果你买的是带粘胶的相册，但你又不愿意将剪纸粘死，可以用透明的玻璃纸将剪纸同粘胶分离。

The investigation of two years showed that corn ear rot by F.moniliforme was the dominant corn ear rot,and F.moniliforme was the major pathogen to corn ear rot. When the corn was harvested,the pathogen of the corn ear rot were isolated from diseased kernel in Duoying farm in Yaan, Sichuan.

玉米收获时，从雅安市多营农场采集患有典型症状的串珠镰刀菌性穗腐病的果穗，用患病病籽粒分离得到病原物，经过鉴定，确定其为串珠镰刀菌，作为本实验的实验菌株，冰箱保存备用。

Methods Indirect immnofluorescence assay was applied to identify specificity of the two groups of monoclonal antibodies prepared with crude antigen and recombinant antigen of Aspergillus fumigatus,respectively.Two different double monoclonal antibody sandwich ELISA assays established with the two groups of antibodies were performed to detect antigents in the cell culture supermatants of 19 common species of Aspergillus,Penicillium marneffei,and 5 species of Candidas.

采用天然烟曲霉抗原免疫，获得广谱针对曲霉抗原的单克隆抗体；采用重组烟曲霉抗原获得特异性针对烟曲霉抗原的单克隆抗体，用间接免疫荧光鉴定，并分别建立2种双抗体夹心ELISA法，对19种常见的环境和临床分离曲霉株、马尔尼菲氏青霉菌及念珠菌培养液进行检测。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。