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The 1-propanol/biuretium flour protein fractionation procedure based on the differential solubility of wheat flour protein in different aqueous solutions of 1-propanol yields three protein fractions, one monomeric in nature and two polymeric fractions corresponding to soluble glutenin, insoluble glutenin. Quantitative of the fraction was made with improved biuret reaction.

丙醇—双缩脲改良测定法用不同浓度的丙醇溶液将小麦面粉蛋白质分离为功能截然不同的单体蛋白、可溶性谷蛋白和不可溶性谷蛋白三部分,然后通过改良双缩脲测定法对不同组分进行定量分析。

METHODS: Human bronchial epithelium cells were isolated and cultured from 18 patients undergone pueumonectomy in hospital. Interleukin 4 or interleukin 6 at concentration of 10 μg/L was used to treat these cultured cells, and Iamil was measured by whole cell patch clamp techniques.

体外分离培养气道上皮细胞,经过终浓度均为10 μg/L的IL-4、IL-6处理,用全细胞膜片钳技术记录原代培养的气道上皮细胞中Iamil。

Methods A routine culture system of melanocytes was established, cells were irradiated with different dosages of UVB(30 and 90mJ/cm2 respectively), and then incubated with different concentrations of TMP for 72h. MTT method, NaOH assay and DOPA oxidization reaction were employed to determine the cell proliferation, melanin synthesis, tyrosinase activity of melanocytes.

常规分离培养正常人黑素细胞,分别用30mJ/cm2、90mJ/cm2的UVB照射细胞,然后加入100μg/ml、300μg/ml、600μg/ml的TMP孵育至72h,采用噻唑蓝法测定细胞增殖情况;多巴氧化法测定酪氨酸酶活性;NaOH裂解法测定黑素含量。

Rock a drill barrow in planting;plant with a drill barrow

把去盖的蜂房放在特定装置中转动,用产生的离心力使蜂蜜分离出来

An elution process is monitored at the position of a wavelength of 363nm on an ultraviolet spectrophotometer to collect a solution at a first wave crest and after the solution is cooled and dried for 22 hours to 28 hours, folacin coupled chitosan solid is obtained.

将反应后的产物冷却至室温,用凝胶柱进行分离,在紫外分光光度计上,于363nm波长处监测洗脱过程,收集第一个波峰的溶液,将其冷冻干燥22~28小时后,得到叶酸偶联壳聚糖固体。

The results were as follows sperm vigor parameters were all decreased in 12 patients, whereas, pattern parameters had no singnificant difference. By SEM, the results were as follows: infertility group acrosomes were much more bigger than the normal ones.

结果发现,不育症精子的运动参数:活率、活动力、平均径速度明显下降,而运动方式参数直线分离度却无明显差异,进一步用扫描电镜观察其头颈部,特别是顶体的超微结构,发现不育症精子顶体较大。

We cut out the skin tissue before separate the tissue of orbital septum by bistouries that can reduce operative reaction and advance the veracity of cutting out skin tissue and shorten the operative time. It is a good method for clinical application.

睑袋整形术中,用手术刀在分离、去除眶隔脂肪前切除皮肤组织,可以避免因麻醉及手术引起的组织反应较重后去除组织的不准确,并且一次性切除,缩短了手术时间,值得临床推广。

Methods: Dorsal root ganglions were derived from the chick embryo and dispersed into single neuron cell,The rhCNTF was added to neuron cells and incubated for 64 hours,The activity of acid phosphatase in neuron cells was determined and the biological activity of rhCNTF was analyzed quantificationally.

从鸡胚中分离出背根神经节并制成神经细胞,将重组人睫状神经营养因子加入到细胞中继续培养64h后,用酸性磷酸酶法检测活细胞内酸性磷酸酶的活性,从而定量测定重组人睫状神经营养因子的生物活性。

Using an anticoagulant it was possible to isolate plasma and intact haemocytes from Helicoverpa armigera larvae. PO and ProPO activities in plasma and haemocyte lysate were assayed when using bovine trypsin as an activator and L-DOPA as a substrate.

用抗凝剂从棉铃虫血淋巴中分离获得了血浆及完整的血细胞,以L-DOPA为底物,牛胰蛋白酶为激活剂,测定了血浆及血细胞裂解液中酚氧化酶及酚氧化酶原的活性。

The European system will try to intercept missiles in mid-course in space, where warheads separate.

在欧洲的反导系统将用来拦截片刻将至其弹头会分离的中程导弹。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。