分离用的

The invention discloses a method for extracting polysaccharide from chiretta and an application, which comprises breaking dry chiretta via a breaker into powder, arranging into an extraction pot, adding ligarine to reflux and defat, adding distilled water into defatted residues to extract, processing alcohol precipitation and centrifugation to obtain deposits, removing protein, processing acid hydrolysis and gel chromatography separation, freezing and drying to obtain active chiretta polysaccharide.

本发明公开了一种从当归中提取多糖的方法及应用。干燥当归用粉碎机粉成粉末，置入提取罐中，加入石油醚回流脱脂，向脱脂后的残渣中加入蒸馏水提取，醇沉、离心得沉淀，除去蛋白、酸解、凝胶色谱分离、冷冻干燥得到活性当归多糖。

By n-butanol extraction, ammonium sulfate fractionation and sodium chloride dialyzation, the partially purified enzyme was gained by means of gel filtration on sephadex G-150 column, and some properties of the enzyme were determined.

方法］以DFRKN-1为提酶材料，经正丁醇提取，硫酸按分级沉淀分离，透析获得酶的粗提取液，用SephadexG-150凝胶过滤柱层析纯化，获得纯化碱性磷酸酶，并测定纯化碱性磷酸酶的理化性质。

AIM: To investigate the effect of PD98059, a specific repressor of extracellular signal regulated kinase1/2 (ERK1/2), on the proliferation of the murine lymphocytes. METHODS: Lymphocytes were isolated from lymphoid nodes of BALB/c mouse. After stained with carboxy fluorescein diacetate, succinimidyl ester, the cells were stimulated with polyclonal activators, concanavalin and phorbol 12,13 dibutyrate plus ionomycin.

分离小鼠淋巴结细胞，藉羧基荧光素乙酰乙酸染色，用多克隆刺激剂刀豆A蛋白或者佛波醇酯加离子霉素刺激，以流式细胞术分析PD98059对淋巴细胞增殖的影响；利用碘化丙锭染色分析PD98059对细胞周期的影响。

Objective To explore the effect of different anticoagulants on quantitative assays for HBV DNA using real-time PCR technique. Methods Peripheral blood samples were obtained from 40 patients infected with HBV. Serum and plasma samples from three kinds of anticoagulants such as heparin, trisodium citrate, and dipotassium EDTA were stored at -20℃.

目的 探讨用实时荧光定量PCR技术检测乙型肝炎病毒DNA拷贝时，不同抗凝剂对乙型肝炎病毒DNA定量检测的影响方法采集40例乙型肝炎患者血液，分别置于不含抗凝剂及含肝素钠、枸缘酸钠、EDTA-K2抗凝剂的无菌试管中，分离血清和血浆后于-20℃冰箱保存备用。

In order to carry out the separation of the azeotropic mixture dipropyl ether + 1-propyl alcohol by extractive distillation, we are studied the influence on the phase equilibria behavior four different solvent: 1-pentanol [2], butyl propionate [3], N,Ndimethylformamide [4] and 2-ethoxyethanol.

为了用萃取性蒸馏法分离二丙醚和1－丙醇组成的共沸混合物，我们正在研究4种不同溶剂对相平衡行为的影响：1-戊醇[2]、丙酸丁酯[3]、N，N－二甲基甲酰胺[4]和2-乙氧基乙醇。

The extraction and separation of total alkaloids from Wu-He Dipsacus by continuous extraction are described. Then the analysis of the quantity of alkaloids is carried out by UV method.

采用索氏提取法对不同生长时期的五鹤续断中总生物碱进行提取并分离，探讨用紫外分光光度法测定总生物碱质量分数的方法。

The extraction and separation of total alkaloids from Wu-He Dipsacus by continuous extraction are described.Then the analysis of the quantity of alkaloids is carried out by UV method.Furthermore,the determination method of alkaloids is discussed.

采用索氏提取法对不同生长时期的五鹤续断中总生物碱进行提取并分离，探讨用紫外分光光度法测定总生物碱质量分数的方法。

Based on the trait of adsorption and desorption of chicken red blood cell with AIV under some conditions, virus enrichment was performed in 48 samples diagnosed by CRBC, and the enrichment products were detected by the multiplex RT-PCR In whih, 30 of 48 were showed to be H5 subtype AIV, completely accorded with the result of same samples tested by embryonated eggs propagation and hemagglutinin inhibition assay.

结合AIV能够吸附鸡红细胞并在一定条件下解离的特点，利用鸡红细胞对48份已确诊样品进行病毒富集，然后用建立的多重RT-PCR方法检测富集产物结果显示48个样品中，30个为H5亚型AIV阳性，与病毒分离结果完全一致。

Three strains of bacteria were isolated from haemolymph, hepatopancreas, ascites and muscle of the diseased Eriocheir sinensis caused by aeromonas. All of the three isolates were Gram-negative rods, motile by means of single polar flagella, and were facultatively anaerobic; Glucoce was catabolized with production of acid and gas; Oxidase was positive.

利用硫酸铵盐析、Sephadex G-100凝胶层析和DEAE-Cellulose离子交换柱层析等方法，从其胞外产物中分离纯化出了2种胞外蛋白酶，用SDS-PAGE电泳测得两种蛋白酶的分子量分别为39.6kD和20kD.39.6kD蛋白酶活性在50℃～60℃范围内热稳定性最好，最适pH为9，而20kD蛋白酶的最适温度为50℃，最适pH为8。

Binders are helically applied colored thread, yarn, or plastic ribbon. They're used to confine, separate and identify groups of fibers or wires in a cable.

绑带就是螺旋形绑定这些导线的彩线、纱线或塑料绳，用来定义、分离和识别电缆中的光纤或电线组。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。