分离用的

Methods］The total proteins of untreated and ethambutol treated mc2155 cells were separated by ampholyte pH gradients based two-dimensional gel electrophoresis (2-DE). The differential expression proteins were analyzed by using image analysis software.

方法］采用载体两性电解质pH梯度双向凝胶电泳技术分离乙胺丁醇处理前后耻垢分枝杆菌mc2155细胞的总蛋白质，用PDQuest 7.3.1图像分析软件比较分析，以识别差异表达的蛋白质。

Filter the separated aqueous layer, wash the diphenyl ether with 5.0ml of ice-cold water, pass the washings through the filter, and collect the filtrate and washings in a 25-ml volumetric flask.

过滤已分离的水层，用5.ml的冰水洗涤二苯醚，合并滤液和洗涤液。

A method to finely separate saturated hydrocarbon and aromatic hydrocarbon by high performance liquid chromatography was introduced.

该文介绍了用高压液相色谱对族组分中的饱和烃、单环芳烃、二环芳烃及三环以上芳烃的精细分离方法。

METHOD: tetracaine was isolated from plasma and cerebrospinal fluid impurity by RP-HPLC.

用RP-HPLC法将地卡因同人血浆和和脑脊液中的杂质分离。

The hydraulic diameter of polymer molecular and the inaccessible pore volume in rocks of different permeability are measured by using the electrical resistivity method.

采用汞压仪和AMT—2400孔径分析仪，研究纳米硬质合金粉末&桥接&团粒对粉末及其压坯中孔隙体积分布曲线的影响。用沉降分离、高能剪切粉碎。

The germinal vesicle can beisolated successfully in such a physical method and it can undergo chromatincondensation in vitro just as the oocyte does in vivo.

用直接取核法可成功分离出游离的生发泡，且它们在体外培养条件下可以和体内一样发生染色质凝集现象。

Methods Streptomycin and dihydrostreptomycin were extracted from honey samples using a liquid extraction with a phosphate buffer followed by a solid-phase extraction based on weak cation-exchange columns. After sample preparation, the hydrophilic interaction chromatography was used to retain streptomycin and dihydrostreptomycin on the column for the separation of the analytes from endogenous compounds. The aminoglycosides were detected and quantified with electrospray ionization tandem mass spectrometry.

方法用磷酸盐缓冲溶液提取蜂蜜中的链霉素和双氢链霉素后，经弱阳离子交换柱进行固相萃取，样品制备后采用亲水作用色谱，使链霉素和双氢链霉素实现保留，从而与内源性物质达到分离，采用电喷雾离子化串联质谱法检测和定量。

As support, Agarose can benot only used in normal condition, but also in denaturalization (for example, 6 mol/Lhydrochloric acid carbamidine or 8 mol/L urea). Herein, the preparation of IMACmatrixes were conducted using agarose as support, carboxymethylated aspartate as chelating ligand and Co~(2+) and Ni~(2+) as chelating center ions.

本实验用琼脂糖作为基质，选用四配位，与金属离子有较强作用力的羧甲基天冬氨酸为配基，以Co~(2+)、Ni~(2+)作为螯合金属离子，合成了一系列金属螯合亲和色谱填料，并对其蛋白质分离性能进行了研究。

Methods Retrospective analysis was perform to analyze 2138 blood samples using the automated BacT/Alert3D culture system. The isolated bacteria ere identified using the API system.

采用回顾性分析，对BacT/Alert3D全自动快速血液细菌培养系统培养的2138份血液标本进行检测，分离所得菌株用VITTEK32全自动微生物分析／药敏系统进行鉴定和药敏试验。

The results indicated that these isolatesof Newcastle disease virus were high virulent Newcastle disease virus.

用23株分离的鸡新城疫病毒接种30日龄新城疫非免疫鸡，其发病率为100％，病死率为70％～100％。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。