分离用的

METHODS: Eight fresh patellae specimens were divided into two groups at random, AO steel wire group (n = 2) and absorbable tension band group (n = 6) to create models of transverse fractures, which were separately fixed by absorbable tension band, steel wire tension band + Kirschner wire to measure their biomechanics function. If fracture gap reached 1.0 mm, the fixation failed.

随机分成AO钢丝组2个和可吸收张力带组6个，制成横断骨折模型，分别用可吸收张力带、钢丝张力带加克氏针进行内固定，测定它们的生物力学性能，骨折端分离1.0 mm为固定失效。

Langone JJ (1982) Protein A of Staphylococcus aureus and related immunoglobulin receptors produced by streptococci and pneumococci.

其中，醛基免疫磁性载体的捕获和分离效果最佳，且用乙醛—右旋糖苷进行包被可以消除交叉反应。

METHODS: In mobilization group (M, n=10), granulocyte-colony stimulating factor (30 μg·kg-1·d-1) was injected subcutaneously 3 hours after MI and every 24 hours for 5 days. On the 5th day, the BMCs from 10 mL peripheral blood were labeled with bromodeoxyuridine for 24-48 hours, then reinjected intravenously. In transplantation group (T, n=10), BMCs transplantation was performed 5-7 days after MI.

将30只新西兰兔采用结扎前降支的方法复制心肌梗死模型，随机分为动员组、移植组和对照组，动员组（n=10）心梗后3 h开始皮下注射粒细胞集落刺激因子30 μg·kg-1·d-1，连续使用5 d，第5 d抽取静脉血约10 mL，分离单个核细胞用5-溴脱氧尿嘧啶核苷标记后，经静脉注入动物体内。

Samples were saponified with 20 g/L sodium hydroxide. The sexual hormones were then extracted with dichloromethane-acetic acetate (40∶1, v/v) under acidic conditions (pH 3, adjusted with 1 mol/L HCl ). An XTerraTMRP18 column was employed and a mixture of water-methanol-acetonitrile (50∶32∶18, v/v) was used as mobile phase. The seven sexual hormones were detected at 230 nm. The average spiked recoveries for the seven sexual hormones ranged from 75.6% to 97.8% with relative standard deviations of 1.9% to 7.2%. The linear ranges of determination were from 5 to 50 mg/L with correlation coefficients of 0.9999, and the limits of detection were from 3.7 to 12 ng.

先在试样中加入20 g/L氢氧化钠溶液与油脂进行皂化反应，然后用二氯甲烷-乙酸乙酯(体积比为40∶1)混合液在酸性条件下(pH 3)萃取，选择XTerraTMRP18色谱柱，以水-甲醇-乙腈(体积比为50∶32∶18)混合液为流动相，在波长230 nm处检测。7种性激素分离良好并排除了样品中杂质峰的干扰，低、高浓度平均回收率范围为75.6%~97.8%；相对标准偏差为1.9%~7.2%；检出限为3.7~12 ng。

The scrap processor buys this raw material, segregates it, and. prepares it into specified grades that consumers -- steel mills and foundries -- buy for melting.

废料处理者购买这种原料，将它分离，把它整理成为规定的等级，使买方——钢厂和铸造厂购买用来熔炼。

Water was syringed between lens nucleus layers to free the lens from its layers and extract it from the little incision,and fulfiled lens crush and liberation.

用钝头针插入晶状体核层间注水，使硬核周围的核壳层层分离，游离出硬核并将之溢出切口，完成碎核、娩核。

The latter is chlorinated in the side chains under free-radical conditions to give 2,4-dichloro-5-fluoro-3-dichloromethyl-1-trichloromethylbenzene. The latter is hydrolysed via 2,4-dichloro-5-fluoro-3-dichloromethylbenzoic acid, which can be isolated if necessary, to give 2,4-dichloro-5-fluoro-3-formyl-benzoic acid, the aldehyde group of which is reacted to give 2,4-dichloro-5-fluoro-3-N-hydroxyiminomethyl-benzoic acid, from which, with simultaneous conversion of the carboxyl group into the chlorocarbonyl group, water is eliminated using an acid chloride to give the nitrile 2,4-dichloro-3-cyano-5-fluoro-benzoyl chloride.

随后，在游离基条件下进行侧链的氯化，得到2，4-二氯-5-氟-3-二氯甲基-1-三氯甲基苯、后者被水解，经过2，4-二氯-5-氟-3-二氯甲基苯甲酸(如果需要此化合物可以分离)，得到2，4-二氯-5-氟-3-甲酰苯甲酸，将其醛基反应，得到2，4-二氯-5-氟-3-N-羟基亚氨基甲基苯甲酸，用酰氯从其中除去水，同时将羧基转化为碳酰氯，得到一种腈，即2，4-二氯-3-氰基-5-氯-苯甲酰氯。

Its extraction, stability and the reaction with amino acid were studied.

结果表明，用板层析方法可分离、制备较纯的橙色素。

The reaction between antigen and antibody was carried out by one step balance method and incubated in 4℃ for 24 hours, then separated bond and free antigen by PR reagent.

用氯氨T法制备125I标记IL-6，经Sephadex G-25纯化，抗原抗体反应采用平衡一步法，4℃温育24 h后经PR试剂分离结合和游离的标记抗原。

The reaction betwe en antigen with antibody was carried out by one step balance method and cultured i n 4℃ for 24 h,then separated binding and free antigen by PR reagent.

用氯氨T法制备125I标记IL-8，经Sephadex G-25 纯化，抗原抗体反应采用平衡一步法，4℃培养24 h后经PR试剂分离结合和游离的标记抗原。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。