分离用的

Highly purified and viable human nasoseptal chondrocytes were obtained by means of mechanical dissection and enzymatic digestion with collagenase type Ⅱ.

1。用机械分离和胶原酶消化方法可得到高纯度和高活性的人鼻中隔软骨细胞。

The dissertation consists of six chapters: Chapter 1 provides a review on the mechanism, the different method and application of nonaqueous capillary electrophoresis.

首次用毛细管电泳方法分离和测定了某些藏药中几类活性成分，并建立了鉴别龙胆科藏药和中药的新方法； 3。

Technological extrction process of f;avonoid compound with organical solvent was experimented .

研究了用氧化铝、硅胶薄层分和柱状色谱分离银杏叶提取液的工艺条件。

Taking advantage of the techniques of osteoblastic culture, we can observe the direct effect of TKR on osteoblast.

取第3代细胞，培养24h，换含不同分离组份的培养液，继续培养72h，细胞增殖检测用MTT法。

These methods are useful to se parate the constituents of stellera chamaejasmel.

用此实验方法可有效地分离瑞香狼毒的化学成份。

Methods DCs were prepared from peripherad blood mononuclear induce d with gra-nulocyt e/macrophage colony-stimulating factor and interleukin-4. Apoptosis of glioma cells were induced with γ-radiation. We design these experiment groups including (1) coculture of DCs and apoptotic glioma cells and T cells,(2) coculture of DCs and U937 cells and T cells,(3) coculture of DCs and cultured glioma cell and T cells,(4) coculture of Des and T cell.

用粒－巨噬细胞集落刺激因子加白介素－4(IL-4)从人外周血分化、诱导DCs、γ-射线在体外诱导培养的人脑胶质瘤细胞凋亡，将DCs、T淋巴细胞和凋亡胶质瘤细胞共培养，同时设计不同类型肿瘤细胞（U937及培养胶质瘤细胞）作对照，分离、富集DCs、T淋巴细胞进行免疫应答及肿瘤细胞杀伤试验。

Methods DCs were pre pared from peripherad blood mononuclear induce d with gra-nulocyt e／macrophage colony-stimulating factor and interleukin-4.Apoptosis of he patochlangioma cells were induced with γ-radiation.We design these experiment groups including(1)coculture of DCs and apoptotic cancer cells and T cells,(2)co culture of DCs and necrosis cancer cells and T cells,(3)coculture of DCs and cul tured cancer cell and T cells,(4)cocultu re of DCs and T cell,(5)cocuture of DCs and cultured cancer cell.

用粒-巨噬细胞集落刺激因子加白介素-4(IL-4)从人外周血分化、诱导DCs、γ-射线在体外诱导培养的人胆管癌细胞凋亡，将DCs、T淋巴细胞和凋亡胆管癌细胞共培养，同时设计不同类型肿瘤细胞(坏死胆管癌细胞及培养胆管癌细胞)作对照，7d后，分离、富集DCs、T 淋巴细胞进行免疫应答及肿瘤细胞杀伤试验。

Planococcus can metabolize electric insulating oil.

对动性球菌代谢变压器绝缘油进行了研究，发现动性球菌能够代谢绝缘油，利用薄层层析、液-质联机等技术从变压器绝缘油的动性球菌代谢物中分离得到一种脂肪酸，用质谱、核磁共振对其结构表征。

Calcium humic acid resin is synthesized by one step gel method. With the column filled with the resin made,the column poiser kept at pH 5～6, and with 0.01 mol/L HCl as an eluent, Ca 2+, Mg 2+ can be separated quantitatively.

利用一步凝胶法合成了钙型腐植酸树脂，用自制树脂装柱，控制柱平衡液的pH为5～6并以0.01mol／LHCl为洗脱液，使Ca2＋、Mg2＋离子与其它12种离子定量分离。

The method has been applied to preconcentration, separation and determination of trace Pt and Pd in mineral samples.

方法用於矿石中微量铂、钯的富集分离和测定，结果与等离子体发射光谱法及推荐值相符。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。