分离用的

The VP2 gene of Canine parvovirus isolated from a raccoon dog was amplified by PCR and cloned into the vector pMD18-T.

用PCR方法扩增犬细小病毒(Canine parvovirus，CPV)貉分离株(PV/貉/CC/1/86)的VP2蛋白基因，并将其克隆入pMD18-T，命名为pTCPV，进行测序分析。

Numerical simulations of laminar flow separation and reattachment occurred in an annular backstep passage are performed by the SIMPLER algorithm.

用SIMPLER算法对流过环形后台阶通道内的层流分离和再附着进行了数值模拟。

Methods Split the recombinant E. coli for expression of rhOP-1 by ultrasomication in ice bath and extract the inclusion body. Dissolve the inclusion body with 8mol/L urea, purify by SP-Sepharose chromatography, refold by gradient dialysis.

将表达rhOP-1的大肠杆菌菌体在冰浴下超声裂解，分离提取包涵体，用8mol/L尿素溶解，纯化后进行梯度透析复性。

One SFG rickettsia strain,named as NH 95,was isolated from Haemaphysalis yeni collected in Ninghua county and was compared with the prototype strains and Chinese reference strains of SFG rickettsiae by SDS PAGE,Western blot and PCR/RFLP.

并从宁化县收集的越原血蜱中分离到一株斑点热群立克次体，命名为NH－95株，分别用SDS－PAGE、Westernblot及PCR／RFLP进行了鉴定。

Savary used method of separation medium fungi, France BIOMERIEUX application of automatic identification systems on the EXPRESSION fungi for biological and chemical identification and drug sensitivity.

用沙氏培养基分离真菌，应用法国BIOMERIEUX的EXPRESSION自动鉴定系统对真菌进行生化及药敏鉴定。

The outer seed capsule system solvent extracts of ginkgo refers to separating methanol extracts with various organic solvent system, and obtaining different solvent extract.

银杏外种皮系统溶剂提取物，是指用各种有机溶剂系统对银杏外种皮甲醇提取物进行分离而制出不同溶剂的提取物。

The lateral displacement of lattice shell is obtained by separation of variables for fixed boundary condition.

在周边固定条件下，用分离变量函数法给出网壳的横向位移。

Now, to obtain this result, it had to be treatedeither with soda or lime.

现在需要用碱化的方法把甘油从脂肪里分离出来。

Samples of bacterium taken from Huguangyan Maar Lake are isolated and purified using spread plate method and streak plate technique. The partial characteristics is analyzed morphologically and physiologically.

采用涂布法、划线分离法以及形态学和生理生化特性分析，对湛江湖光岩微生物进行了分离纯化及部分性质研究；同时用平板菌落计数法对其异养细菌、真菌和弧菌等的数量进行初步统计。

Methods: Glipizide gliclazide glyburide glimepiride gliquidone repaglinide nateglinide pioglitazone rosiglitazone metformin phenformin and tolbutamide added in the blank ground samples were extracted with 80% methanol and were separated from an ACQUITY UPLC DBH C18 column with a gradient mobile phase of acetonitrile - 0.2 % formic acid water solution. A tandem quadrupole mass spectrometer equipped with electrospray ionization source was used in positive ion mode multiple reaction monitoring was performed to quantify above mentioned compounds.

被添加在中成药制剂及其包装物中的二甲双胍、苯乙双胍、罗格列酮、吡格列酮、格列吡嗫娶瑞格列奈、甲苯磺丁脲、格列齐特、格列苯脲、那格列奈、格列美脲、格列喹酮等西药成分用80%甲醇提取，以乙腈- 0.2%甲酸水溶液为流动相梯度洗脱，在ACQUITY UPLC DBH C18色谱柱上分离，串联四级杆质谱检测，MRM方式采集并定量。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。