分离用的

Methods Silica gel column chromatography and PTLC were used to separate and purify the compounds from the petroleum ether soluble fraction of ethanol extract.

方法用硅胶柱色谱和制备薄层等方法从乙醇提取物的石油醚萃取部位分离化合物，通过波谱方法鉴定其结构。

Methods After ethyl alcohol extraction, the sample was separated by GC FID detector.

样品经乙醇提取后用具有FID检测器的气相色谱仪分离测定。

And 4 of experimental group and 2 of control group were killed at 1, 3, 5, 10, 14, 20, 25 and 30 days postinfection, respectively. All the sera, the lymphocyte suspension of peripheral blood, thymus, Fabricius bursa and spleen, were collected.

本研究用AEV-NH937株组织毒1∶100倍稀释，0.03ml／只脑内接种1日龄SPF雏鸡，分别在攻毒后1、3、5、10、14、20、25和30天迫杀实验攻毒组4只，对照观察组2只，分离血清，制备外周血、胸腺、法氏囊和脾脏的淋巴细胞悬液，同时采集脑组织，10％福尔马林固定，制备石蜡切片，做H.E。

With water as urea solvent and an organic chlorine compound as activator, the adducts formed into small spheres which are easily filterable.

用尿素饱和水溶液，有机氯化物为活化剂，生成的尿素-正烷烃络合物成球状，易於过滤分离。

This paper presents the method to separate the major components from hawthorn by ultrasonic and macroporous resin.

介绍了用超声提取及大孔树脂分离山楂中主要成分的方法。

Methods:(1) Bone marrow stromal cells isolated from Beagle dogs were cultured in vitro for the primary culture,in basic medium consisted ofDMEM^iew horning cattleerum,etc. Parts of subculture were cultured in mineralization medium which additionally contained 50 y g/ml ascorbic acid, 10mmol/LNa- beta -Glycerophosphate and 10-8mol/L dexamethasone in order to induce cells differentiating into osteoblasts.

①抽取毕格犬髂骨骨髓体外分离培养获得骨髓基质细胞，DMEM、新生牛血清培养基进行原代培养，部分传代细胞以含10~(-8)mol/L地塞米松、50μg/ml抗坏血酸和10mmol/Lβ—甘油磷酸钠的矿化培养液诱导其向成骨细胞增殖分化，用倒置相差显微镜进行细胞形态学和细胞生长增殖观察。

The natural or dry process, where the cherries are dried in the sun or in dryers, and the fruit is then separated from the bean by processing them through a mechanical husker.

有两种脱去外皮的方法：自然法，也秤干法；即晒干或烘干后用机械方法分离。

The discrete stiffener solution and orthotropic plate solution are used for analyzing the buckling behavior of intermediately stiffened plates theoretically in this paper. Thus, closed-form solution of critical buckling stress and required adequate stiffening rigidity of the stiffener is obtained.

本文分别用分离加劲肋法和各向异性板法对纵向中间加劲板件的屈曲性能进行了理论分析，得到了屈曲应力和理论充分加劲刚度解析表达式。

Strains M110 and M113 isolated from Aedes albopictus and Armigeres durhami, respectively, which were fatal to suckling mice at 4 days after intracerebrally inoculation and had cytopathologic effects on BHK21 cells, showed a positive reaction to dengue antibody and could be amplified Ly using flavivirus primers and dengue type-4 NS1 gene primers and dengue type-4 NS2a gene primers.

结果 从白纹伊蚊和达勒姆阿蚊中各分离到一株病毒，分别命名为M110和M113，这两株病毒对BHK21细胞能产生明显的细胞病变，脑内接种乳鼠4d左右导致乳鼠死亡。该病毒经血凝、血凝抑制和间接免疫荧光试验提示为黄病毒。分别用黄病毒属特异引物、登革4型病毒NS1和NS2a基因片段特异引物进行RT-PCR扩增、序列测定，证实为登革4型病毒。

An orange-red crystalline substance was isolated from the chloroform extract of red callusdry powder by silica column chr

从干燥的红色愈伤组织中，用氯仿提取、硅胶柱层分离到一种橙红色结晶，经鉴定为隐丹酮。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。