分离用的

Cloning of the Schwanniomyces occidentalis α-amylase and high expression in S.cerevisiae.The E.coli / yeast shuttle plasmid YCEpl partial library of Schwanniomyces occidentalis DNA was constructed and α-amylase gene fragments were screened in Saccharomyces cerevisiae by amylolytic activity.Several transformants with amylolysis were obtained and one of the fusion plasmids has about 5.0 kb inserted DNA fragment.It contains the upstream and downstream sequences of α-amylase gene from S.occidentalis .

以E.coli/yeast穿梭质粒YCEp1为载体构建西方许旺酵母部分基因组文库，在大肠杆菌中扩增后提取混合质粒DNA，经电转化非缺陷标志酒精酵母 AS.2.1364，在YPDS平板(含1%葡萄糖和1%可溶性淀粉)上用淀粉水解活力筛选含水解淀粉的阳性转化子，从阳性转化子中分离重组质粒证实含5.0kb的插入片段，用α-淀粉酶基因两端序列设计的引物PCR扩增及扩增片段序列分析证实该片段中含有α-淀粉酶全部编码序列。

The distribution coefficients of rubidium and caesium between Cation-42, a phenolic-sulfonated cation exchange resin manufactured in Shanghai, and nitric acid as well as nitric acid-methanol or butanol mixtures have been determined by the equilibrium method.

本文用平衡法测定铷、铯在稀硝酸或稀硝酸-甲醇与华东化工学院阳-42号树脂间的分配系数，比较用硝酸及硝酸-醇作淋洗液分离铷、铯的效果，并确定0.47M硝酸是较好的淋洗液。

The joint stock system is a form of capital organization of modern enterprises, which is favorable for separating ownership from management and raising the efficiency of the operation of enterprises and capital. It can be used both under capitalism and under socialism.

股份制是现代企业的一种资本组织形式，有利于所有权和经营权的分离，有利于提高企业和资本的运作效率，资本主义可以用，社会主义也可以用。

Methods: NGF was prepared from mouse submaxillary glands by the way of elution with CM 52 column. The molecular weight and purification of NGF were detected by SDS-PAGE polyacrylamide gel electrophoresis.

以小鼠领下腺为原料，採用CM52多次洗脱的力法分离神经生长因子，SDS-PAGE电泳检测NGF的分子品质和纯度，採用鸡胚背根神经节培养方法检测NGF的生物活性。

In order to obtain pockety aperture, this paper prepares inorganic membrane with choosing diatomite of TengChong. The quality of this choosing diatomite is alterd by adding argentic oxide to improve its sorption capacity of ammonia and separate effect of ammonia water. Diatomite is choosed through the experiments of ultrasonic.

本文用精选后的腾冲硅藻土来制膜，力求使膜的孔径分布均匀，同时用氧化银来改性硅藻土来改变硅藻土对氨的吸附能力，提高氨／水分离效果。

From the ester n, the intermediate Ⅲwas produced by the Sonogashira coupling reaction with terminal alkynes under the co-catalysts of Pd2 and Cul. These final products of alkynyl benzamide derivatives IV were obtained by the cleavage of support with primary or second amines.

液相合成兼容了固相合成可快速简便地对产物进行分离纯化以及溶液相合成可在均相条件下进行反应和用常规手段对反应中间体进行分析检测的优点，用聚乙二醇4000作为可溶性聚合物支持体，通过醚键将[1，3，5]氯三嗪连接在PEG上制备成PEG支持的新型的可溶性聚合物试剂I。

The fact that namespace definitions can be discontiguous means that we can compose a namespace from separate interface and implementation files. Thus, a namespace can be organized in the same way that we manage our own class and function definitions

命名空间定义可以不连续意味着，可以用分离的接口文件和实现文件构成命名空间，因此，可以用与管理自己的类和函数定义相同的方法来组织命名空间

Using PAN as indicator and CuSO4 as indirect titrant the aluminium content is determined.

用氢氧化钠溶液溶解样品的同时将铁与铝分离，加入过量的EDTA与铝络合，以PAN为指示剂，用硫酸铜标准溶液间接滴定法测定铝。

According literature above,its considerd that the former is proper to be interpreted by means of multiple alleles hypothesis,and the fertility heredity of the latter is appropriate to be interpreted using inhibition.

根据大白菜及其近缘芸薹属作物核不育材料育成的纯合两型系和杂合两型系可育株间互交F~1|可育株自交，其子代可能出现无育性分离情况或者产生13∶3两种表型的育性比资料，认为前者宜用复等位基因假说解释，而后者用抑制作用解释为妥。

Methods: Oocytes in the GV stage were separated from ovary by squeezing method. In mouse germinal vesicle GV stage, the expression of ATP8 gene in the mitochondria in the single oocyte was detected by RT-PCR, in which, cDNA was synthesized with two methods: one was the single GV-stage oocyte directly to be placed RT, the other was to perform RT after eliminating mtDNA and nucleus DNA with the EeoR Ⅰ enzyme and Dnase. And the product of RT-PCR was cloned and sequenced.

应用挤压法从卵巢中分离获得生发泡期(germinal vesicle， GV)卵母细胞；用RT-PCR检测GV期单个卵母细胞中ATP8基因的表达：其中cDNA的合成分两种方法进行：一是将GV期单个卵母细胞直接进行RT合成cDNA，二是先用DNA酶加EcoR Ⅰ酶祛除mtDNA和核DNA后再进行RT；回收产物构建克隆质粒并测序。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。