分离用的

At the end of 12 and 18 weeks, rats were killed, taken the muscle of posterior limb, after separating the gastroenemius、soleus、plantaris, to prepare the frozen section of the each kind of muscle respectively to be confirmed to form the myopathy model by the histochemical stain.(3) RT-PCR、Western blot and immunohisto-chemistry stain were respectively used to detect the expression of NF-κB、MMP-2 and MMP-9 during different period.

2实验组用酒精给大鼠灌胃，对照组用含与酒精体积相等的生理盐水灌胃，两组均喂饲改良的全营养高脂饲料，于12周及18周后，取后肢肌肉，在分离出腓肠肌、比目鱼肌、跖肌后，将各类肌肉均分别地制备成冰冻切片和石蜡切片，经组织化学染色确认成模。

Healthy kunming mice mate to produce embryo. sacifice prognant mice at 14dpc, make preparation of fetal liver and born marrow. then proceed 40%-70% percoll gradient seperation to obtain nucleated erythroid cells. resuspend the purified erythroid cells in DMEM medium without serum, adjust cell concentration to 5×10〓.

正常健康昆明小鼠交配，待胚胎发育至14天时，处死怀孕母鼠，取胎鼠肝脏，用另一组成年小鼠制备骨髓；然后应用40％-70％Percoll梯度分离法分离胎肝和骨髓中的有核红细胞，纯化后的细胞用无血清DMEM培养基悬浮，调整细胞浓度为5×〓／ml。

Bis (P—bromobenzylamino) ethane and 1,2—bis (m—Iodobenzylamino) ethane may be prepared by condensation of the corresponding halo benzaldehyde with ethylenediamine and followed by catalytic hydrogenation of the Schiff's bases over Pto_2 in glacial acetic acid solutions: Both Ⅱa and Ⅱb may react in neutral mediam with aldehydes to form well—defined crystalline tetrahydro—imidazole, which may be served as derivatives of identification for aldehydes: Under the same condition, however,Ⅱb may ...

用1，2-二乙烷与二十余种醛类作用，得到有固定熔点的晶体衍生物，它们的熔点分布在85—210℃之间，产率为40—96％，这试剂与甲基酮类在相同条件下无反应，因此，它可作为鉴定醛类的专属试剂。 2。上述衍生物用稀盐酸在室溫处理，能发生水解，可以定量地收回原来的醛，因此这试剂又可作为分离醛类的试剂。 3。上述衍生物可以用非水滴定法半微量地测定其分子量可作为鉴定醛类的另一项参考数据。 4.1，2-二乙烷与醛类能生成晶体衍生物，但这试剂也与甲基酮在同样条件下发生反应，所以不适合作为鉴定醛类的专属试剂。

Methods Firstly, red chilli powder was soaked with organic solvent, then the extract was saponified under different conditions scheduled by temperature, time and solvent content to obtain crude capsanthin which was refined by silica gel chromatography.

方法先用有机溶剂浸提粉碎的干辣椒粉；然后皂化处理浸提物，比较辣椒红色素在不同温度、时间、溶剂浓度下提取的效果；最后用硅胶柱层析进行精制分离。

Methods Firstly, red chilli powder was soaked with organic solvent, then the extract was saponified under different conditions scheduled by temperature, time and solventcontent to obtain crude capsanthin which was refined by silica gel chromatography.

先用有机溶剂浸提粉碎的干辣椒粉；然后皂化处理浸提物，比较辣椒红色素在不同温度、时间、溶剂浓度下提取的效果；最后用硅胶柱层析进行精制分离。

Proton and C-13 NMR spectroscopy analysis assessed the carbon aromaticity of the supercriticaly extracted bitumens to be 0.32-0.34, it appears in fair agreement with the aromaticity determined directly by using solid state C-13 NMR techniques.

然后，分别用核磁共振波谱法测定它们的芳碳率，结果分别为0.30和0.34。将超临界流体抽提沥青分离为族组分，用核磁共振与X-射线衍射法分析测定它们芳碳部分的主要结构参数及其聚集态。

The ELISA assay established with the crude antigen-specfic monoclonal antibodies could detect both of the clinical and environmental isolates of Aspergllius, while the other assay could only detect Aspergillus fumigatus of both clinical and environmental isolates.And no cross reaction with the cell culture of Penialllium marneffei and Candidas was observed with the two methods.

用mAbs-1建立的双抗体夹心ELISA法可检测19种常见曲霉株培养液；用特异性针对烟曲霉抗原单克降抗体(mAbs-2)建立的双抗体夹心ELISA法可特异性检测临床和环境分离株烟曲霉培养液；与其他曲霉株无交叉反应；2种双抗体夹心ELISA法与马尔尼菲氏青霉菌及念珠菌培养液均无交叉反应。

Crude solution of chitosanase is obtained by fungal fermented filtrates firstly, and then through affinity adsorption by cross - linked chitosan resin, with no protein flowing by suffer, and then through eluting by 1-5per mill Cu2 . After that, eluent is dialysed and concentrated and then separated by gel filtration chromatography.

将产壳聚糖酶真菌发酵液离心得粗酶液，经交联壳聚糖树脂亲和吸附，用缓冲液洗至无蛋白流出，再以1-5‰的Cu 2 洗脱，洗脱液透析浓缩后用凝胶过滤层析进行分离，获得电泳纯的壳聚糖酶。

The lung tissues were extracted with acetonitrile and 0.1M potassium dihydrogen phosphate buffers. The extracts purified by C18 solid-phase extraction cartridges. The eluates were determined by HPLC at 288nm. Limits of detection were 0.025 ug/mL for tilmicosin in plasma and 0.025ug/g for tilmicosin in lung, respectively.

采用C18 SPE柱分离和提纯血浆中的替米考星，用乙腈和0.1M磷酸二氢钾缓冲液提取肺脏中的替米考星，用C18 SPE柱净化，均采用反相高效液相色谱测定替米考星，检测波长288nm。

METHODS: After isolated the PBMCs and blood-plasma from adult human peripheral blood by Ficoll-Hypaque centrifugation, the PBMC culture procedure with or without the self-blood -plasma was applied to polarize T cells in vitro , these cells were polarized by PHA(20 mg/L),non-PHA respectively.

用Ficoll-Hypaque梯度离心法分离出正常成人抗凝外周血单个核细胞和血浆，将PBMC置于含或不含有自身血浆环境中，并在植物血凝素(PHA ，2 0mg L)的刺激下进行培养，培养 2 4、48、72h后收获细胞，分别用双色免疫荧光标记，以流式细胞术对CD4+ T细胞表达表面分子CCR3、CCR5进行分析。结果：CD4+ T细胞受自身血浆和PHA刺激后向Th1 Th2两个方向极化，2 4、48、72h后极化率分别达到(13 2 8± 1 5 9)%(12 70± 1 6 5 )%、(17 19± 1 0 3)%(17 5 0± 1 30 )%、(19 49± 2 87)%(18 5 8± 1 49)%；而只有PHA刺激的极化率很低，两组有显著性差异(P 0 1)；Th1∶Th2之比约等于 1。

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。