分离用的

Hydrastine(97.7%) was obtained with extracted rate 65%by extracting the Goldenseal with mixed Solvent;Berberine hydrochloride (99.9%) was obtained by adding hydrochloride acid to alcohol (alcohol∶H 2O=1∶1) solution of the extract and Hydrastine (99.5%) was gained by CLC method with the yield 1%.

原药材用混合溶剂直接提取分离，可得到含北美黄连碱 97.7%的结晶体，提取率达 6 5%；提取物经醇水溶解、酸化，可直接获得小檗碱盐酸盐结晶，纯度达到 99.9%；用柱色谱法，可得到 99.5%的北美黄连碱，收率约 1

The Trypsin inhibitor from Cucurbita pepo var. striaca Seeds was isolated by salting-out and chromatography of Sephadex G-50, the inhibitor activity was detected with BAPNA, the Mw was determined with SDS-PAGE and stablity was studied with different temperature, the side chains of Lys and Cys were modified.

采用分级盐析和Sephadex G-50的方法分离纯化无种皮南瓜籽胰蛋白酶抑制剂，用BAPNA法进行抑制活性的测定，用SDS-PAGE法测定蛋白质的分子量，用不同的温度处理方法对它的热稳定性进行研究，并对抑制剂的Lys和cys进行了化学修饰。

The charomatographic conditions of separation and direct dermination of benzophenone-4(MS40), salizyle acid sodium, salol, benzophenone-3(M40), octyl dimethyl PABA(6007), butyl methoxy dibenzoyl methane(1789), octyl methoxyl cinnamate and octyl salicylate(587) from u-Bondapak C18 Column were studied with methanol-water-THF as elution. A HPLC method for these compounds was founded.

研究了用甲醇-四氢呋喃-水作为流动相，从C18柱上分离和测定羟苯甲酮-5-磺酸(MS40)、水杨酸钠、水杨酸苯酯、羟苯甲酮(M40)、对-二甲酸氨基苯甲酸-2-乙基己酯(6007)、4-叔丁基-4-甲氧基二苯甲酸甲烷(1789)、对-甲氧基肉桂酸-2-乙基己酯、水氧酸-2-乙基己酯(587)等8种紫外线吸收剂的色谱条件，建立了用HPLC测定上述物质的方法。

Shutdown, removed the row fan, fan struck by hand, the sense of rotation of the fan is very flexible, so use flat screwdriver inserted under the roots of the gap fanleaf bend to the will of the fan and fan-Block separation and jumped over the fan, the fan to see in the center of a small arc with several magnet surrounded a round shield-shaped, it is clear that this is the fan motor rotor, while the stator is fixed together with the fan-Block a micro-winding, if the fan and fan blocks together, the micro-coil is inserted to the internal rotor.

关机，将该排风扇拆下，用手拨动扇叶时，感觉扇叶的转动很不灵活，故用平头起子插入扇叶根部底下的缝隙内向上翘，将整个扇叶与扇座分离，翻过扇叶时，看到在扇叶的中心部位有一个用数条小弧形磁铁围成的圆罩形，显然，这就是该风扇电机的转子，而定子是与扇座固定在一起的一个微型绕组，若将扇叶与扇座组合在一起，则该微型绕组正好能插在转子的内部。

Firstly, the expressions of free vibration of moderately thick plates in polar coordinates are derived and the general solutions are obtained by the means of method of separation of variables.

首先，导出了用极坐标系描述的中厚板自由振动板的微分方程，用分离变量法求得其一般解。

It regards the cutting track of virtual surgical knife as the cutting intersection of a polygon prism and a triangular mesh model.

该算法用凸柱体模拟虚拟手术刀对三维网格模型进行切割，根据截骨术的切口类型构造柱体，用此柱体进行切割实现骨块的分离。

Reverse-phase chromatography was employed for chiral separation of tropine acid enantiomers on the cellulose tribenzoate chiral stationary phases coated on a small-particle spherical silica support in an eluant system consisting of a mixture of alcohol and water.

摘 要：以乙醇-水流动相，用纤维素-三作为手性固定相对外消旋体托品酸进行了高效液相色谱手性分离考察了不同比例的乙醇-水流动相、不同流速以及用不同醇类酯化对托品酸酯在手性色谱柱上的色谱行为。

The method of electrolysis of nonaqueous solution could analyze the inclusions accurately through the experiment.

介绍了用金相法研究钢中夹杂物以及用酸性电解液电解分离钢中夹杂物所存在的问题，通过实验证明了采用非水溶液电解法能保证夹杂物分析的准确性。

Another method that has been proposed to identify S.aureus from nonclinical sources employs an immunoenzymatic assay using a monoclonal antibody assay using a monoclonal antibody prepared against S.aureus endo-β-acetyl-glucosaminidase, an enzyme produced by all isolates of this species.

另一种方法，提出了确定非临床源金黄色葡萄球菌从员工的免疫酶检测用单克隆抗体检测用单克隆抗体制备对金黄色葡萄球菌内切β-乙酰氨基葡萄糖苷酶，这种酶产生的所有分离这一物种。

Nodosus Pilin gene, the ovine IL-2 gene with synthesized primers with EcoR1 and BamH1 recognition sequence at 5'and 3'end respectively was amplified and cloned from the peripheral blood of ovine.

从健康绵羊血中分离白细胞，用TRIZOL试剂盒提取绵羊白细胞RNA，以RNA为模板，用设计OviIL-2的引物进行RT-PCR和PCR，扩增出设计有BamHl和EcoRl限制性酶切位点的绵羊白细胞介素2。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。