分离用的

Three developing systems were used for chromatography of TLC. UV 254nm, 366nm and anisaldehyde as a chromogenic agent were used for detecting compounds of these strains.

本研究设计4种内生菌发酵培养基，对89株分离自傣药植物且具有高抗菌活性的内生菌及5株潜在放线菌新种进行摇瓶发酵，发酵液的提取物用3种展层系统作薄层色谱展层，用UV(254nm、366nm)和茴香醛试剂进行检测。

According to the frequently used methods of extraction and isolation of natural products from organic body, the freeze-dried ascidian body was exhaustively extracted with methanol/toluene ( 3 : 1 ) and then the combined extracts were filtered and the solvent was separatelyconcentrated under vacuum.

按照目前比较常用的天然产物提取分离方法，将样品冷冻干燥后，用甲醇：甲苯(3：1)浸泡，收集滤液，减压蒸馏得到的浓缩液用NaNO_3水溶液萃取，收集水相，减压浓缩至NaNO_3晶体析出。

The main job and contribution of this paper are as follows:Based on narrowband signal processing, we propose a new method for FH signal blind detection and blind parameter estimation. Firstly, compute the cross-correlation function of FH signals from multichannels, then we get the cross power spectral density matrixCSDM Secondly, select the hops of FH signals from the CSDM via processing and analyzing the CSDM, meanwhile reduce the influence coming from noise, fixed frequency signal and other interferences; Lastly, estimating the DOA of every hop and according to the DOA, we can separate the hops, get the number of FH signals and their parameters. Propose a new method for designing the threshold in FH signal detection. This method makes it easy to get the threshold by theoretic computing and it can extremely reduce the interference of non-FH signals.Based on broadband signal processing, we propose a new method for FH signal blind detection and blind parameter estimation. Firstly, get the DOA of the signals by wideband spatial spectrum estimation; secondly, separate the signals by spatial filtering using wideband beamformer; Lastly, we propose two methods for FH signal blind detection and blind parameter estimation, one is for several constant hop-rate FH signals whose dwell-time are different and another is for a single FH signal whose hop-rate is alterable.By employing spatial interpolation in broadband signal beamforming, it can reduce the number of antenna elements and corresponding RF modules, A/D converters, etc. It can also eliminate the mutual coupling between antenna elements while getting the narrower main beamwidth and lower sidelobe level.

本文的主要研究工作和贡献如下：●提出了一种基于窄带处理的FH信号盲检测和参数盲估计的实现方法，即首先求出多路信号之间的互相关，进而得到互功率谱密度矩阵，然后通过对CSDM进行对折处理来抑制噪声、定频信号及其他干扰的影响，并将所有属于FH信号的hop(每一跳的驻留时间在时频图上持续的线段)从CSDM中筛选出来，最后用阵列信号处理中的到达方向估计理论在频域中估计出各个hop的方向参数，利用方位信息完成FH信号分离并得到FH信号的数目及其各自的参数集；●提出了一种确定FH信号检测门限的策略，该策略不仅使得检测门限能够通过理论计算得到，而且还显著地抑制了非跳频信号的干扰，从而能有效地检测出FH信号；●提出了一种基于宽带处理的短波FH信号盲检测和参数盲估计的实现方法，即首先利用宽带空间谱测向技术得到接收信号的DOA，然后采用宽带波束形成技术对特定方向进行空间滤波，实现多个信号的分离，最后分别提出了两种FH信号的盲检测和参数盲估计方法，第一种方法适用于空间某个特定方向上存在着几个驻留时间不同的恒跳速FH信号和其他干扰信号的混合信号，第二种方法适用于空间某个特定方向上仅含一个可变跳速的FH信号和噪声的混合信号；●将空间插值的概念和宽带波束形成技术结合起来，提出了一种基于频域处理的宽带恒定束宽的波束形成方法。

In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2～0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%～80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0～0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法，将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合，建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺：胰酶用pH4.5醋酸缓冲溶液提取后，粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5，洗脱缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5)含0.2～0.35mol／LNaCl分两步洗脱，收集激肽释放酶的洗脱峰；DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液：0.01mol／LTris-HCl缓冲液(pH7.5，洗脱液：0.5mol／LNaCl溶液，一次洗脱，收集胰蛋白酶洗脱峰；最后，亲和层析未吸附液用30％～80％硫酸铵分级盐析处理，沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5含1mol／L(NH_4)_2SO_4，洗脱缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5)含0～0.6mol／L(NH_4)_2SO_4，分两步洗脱，收集糜蛋白酶的洗脱峰。

Methods Density gradient centrifugalization combined with adherence method were used to segregate and cultivate rat MSCs. MSCs cultivated to the 3 rd passage were characterized using flow cytometry technique. TGF-β1 with 1, 2 and 5 μg/L were pretreated on MSCs for 8 h, then serum and oxygen deprivation were used to cause analogous ischemia of MSCs in vitro. AnnexinV/PI flow cytometry technique was used to evaluate apoptosis and survival of MSCs.

采用密度梯度离心结合贴壁法分离、培养大鼠MSCs，对培养第3代的MSCs进行鉴定；用1、2、5μg/L的TGF-β1分别预处理细胞8 h，然后用血清剥夺和低氧处理建立MSCs体外模拟缺血性损伤模型，通过流式细胞术评价MSCs的凋亡及存活情况；用Western blot方法检测2μg/L的TGF-β1对Akt和p-Akt表达的影响。

It is nothing else than impurities prenatally inherent in ore that seriously affect the quality of the latter, which is formed as a result of geological vicissitudes including diastrophic movement, eruption of volcano, sedimentation, glaciation and weathering etc., under the action of which pyrogenic rocks, volcanic complex, aqueous rocks, sedimentary rocks etc., come into being, some of which exist in a stage of symbiosis, the main cause of the absence of pure rocks in nature, wherein lies the reason for the need of separation technology and apparatus, namely, ore-dressing devices and equipment, so far impotent to meet the requirements of metallurgical industry the scientists make every endeavor to elevate to a new high by laser separation.

没有什么比矿石形成之前就存在的杂质对矿石品质的影响更为重大。矿石是火成岩、火山群、水成岩、沉积岩等成形时，在包括地壳运动、火山爆发、沉积、冰蚀、风化等地质变化的作用下而形成的。而有些地质变化在一个发展阶段中存在共生现象。自然界无杂质岩石缺乏的主要原因在于需要分离技术和设备来完成，即：需要选矿设备和装置。而迄今为止，这些设备还难以适应冶金业的需要，虽然科学家们尽一切努力用激光分离技术将冶金业提高到一个新的高度。

Cephalomappa sinensis is a monotypic genus ever-green tree endemic to the calcareous mountainous region in southwest of Guangxi Zhuang Autonomous fresh branches are used as feeding stuff for cattle,horse,sheep,and can make them find the possibility of developing feed additive or medicine for livestock,the chemical constituents from this plant were chemical constituents of its roots had been reported in our earlier continuation of our investigation on the branches of Cephalomappa sinensis,a new norneolignan,named Ceplignan(1),was isolated from ethanol extract through column structure was elucidated on the basis of spectroscopic evidence.

肥牛木(或称肥牛树，Cephalomappa sinensis)为大戟科一单种属植物，为常绿乔木，其分布区狭窄，仅限于广西西南部石灰岩山地[1]。肥牛木为优良木本饲料树种，是牛、马和羊的好饲料，用该植物饲养牛，可使牛肥壮，故有肥牛木的名称[1]。研究其化学成分，目的在于探讨开发饲料添加剂或兽药的可能性。我们曾报道了肥牛木根的化学成分[2，3]，在对其枝条的进一步研究中，又分离得到一个新的降新木脂素，命名为肥牛木素。本文报道其分离方法和结构鉴定结果。

The bonding form in extracted species can be represented by:〓 It was the direct bonding of donors that made the extraction of partially hydrolyzed iron much more effective, and the stereoselectivity of coordination that made the extraction of unhydrolyzed iron depressed, so that the extraction system became selective to the partially hydrolyzed iron .

研究中选择仲辛醇作为协萃剂对ZnSO〓溶液中的Fe-Zn分离进行了进一步的考查，无论对配制的溶液或是真实的ZnS高压浸取液，除铁及Fe-Zn分离的效果都非常好，而且反萃铁时的用酸量与传统的伯胺除铁过程相比降低了10倍以上，该体系是一个较为经济合理的具有一定发展潜力的萃取除铁体系。

The separation of iron from sulphate solutions has been firstly investigated using a mixed solvent system consisting of tertiary amine N_(235 and tributyl phosphate.

首先，采用N_(235)-TBP混合溶剂体系萃取含铁、锌、锰的硫酸盐溶液中的铁，实现铁与锌、锰的分离；然后用P_(204)萃取含锌、锰的萃余液中的锌，达到锌、锰分离的目的。

The separation of iron from sulphate solutions has been firstly investigated using a mixed solvent system consisting of tertiary amine N_(235 and tributyl phosphate. Then, zinc was extracted with di(-2-ethyIhexyl)phosphoric acidP_(204 from the raffinate in order to separate zinc and manganese.

首先，采用N_(235)-TBP混合溶剂体系萃取含铁、锌、锰的硫酸盐溶液中的铁，实现铁与锌、锰的分离；然后用P_(204)萃取含锌、锰的萃余液中的锌，达到锌、锰分离的目的。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。