分离用的

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum.

为了比较大鼠胎血和骨髓中非造血成体干细胞（non-hematopoietic adult stem cells，NASC）体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同，在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓，用Ficoll-hypague分离液分离出单个核细胞后，种植于含10%胎牛血清的DMEM/LG培养液内。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum. The acquired NASCs were subcultured for passage.

为了比较大鼠胎血和骨髓中非造血成体干细胞（non-hematopoietic adult stem cells，NASC）体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同，在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓，用Ficoll-hypague分离液分离出单个核细胞后，种植于含10%胎牛血清的DMEM/LG培养液内。

METHODS: Bone marrow was sterilely separated from human. After heparinization, human BMSCs were harvested using density gradient centrifugation and adherence method. At the fifth passage, BMSCs at 1×108/L were incubated in the 6-well plate and divided into 2 groups. BMSCs in the edaravone group were 50% confluent and incubated in L-DMEM containing basic fibroblast growth factor and fetal bovine serum for 24 hours. After washing in PBS, these BMSCs were incubated in serum-free L-DMEM containing 20 mg/L edaravone for 24 hours. BMSCs in the blank control group were incubated in L-DMEM, supplemented with 10% fetal bovine serum.

无菌抽取的骨髓经肝素化后，采用密度梯度离心法及贴壁筛选法分离获得人骨髓间充质干细胞，传至第5代按1× 108 L-1接种于6孔板内，设立2组，依达拉奉组细胞达50%融合时用含碱性成纤维生长因子、胎牛血清的L-DMEM预诱导24 h，PBS洗涤后再用20 mg/L依达拉奉无血清L-DMEM诱导24 h；空白对照组始终用含体积分数为10%胎牛血清的L-DMEM培养，不加任何预诱导剂和诱导剂。

Methods The resistance of Salmonella isolates were detected by drug susceptibility tests with KirbyBauer diffusion method. PCR was designed to detect tetA, tetB, tetC, tetD, tetE, tetG and tetK. The amplified products were sequenced and alignment analysis was performed.

用KB法测定分离株对四环素等21种抗生素的耐药情况，用PCR方法检测四环素耐药基因tetA、tetB、tetC、tetD、tetE、tetG及tetK，并对扩增产物进行测序。

Methods The resistance of Salmonella isolates were detected by drug susceptibility tests with Kirby Bauer diffusion method. PCR was designed to detect tetA, tetB, tetC, tetD, tetE, tetG and tetK. The amplified products were sequenced and alignment analysis was performed.

用KB法测定分离株对四环素等21种抗生素的耐药情况，用PCR方法检测四环素耐药基因tetA、tetB、tetC、tetD、tetE、tetG及tetK，并对扩增产物进行测序。

Methods resistance of Salmonella isolates were detected by drug susceptibility tests with KirbyBauer diffusion method. PCR was designed to detect tetA, tetB, tetC, tetD, tetE, tetG and tetK. The amplified products were sequenced and alignment analysis was performed.

用KB法测定分离株对四环素等21种抗生素的耐药情况，用PCR方法检测四环素耐药基因tetA、tetB、tetC、tetD、tetE、tetG及tetK，并对扩增产物进行测序。

Methods The resistance of Salmonella isolates were detected by drug susceptibility tests with KirbyBauer diffusion method. PCR was designed to detect tetA, tetB, tetC, tetD, tetE, tetG and tetK.

用KB法测定分离株对四环素等21种抗生素的耐药情况，用PCR方法检测四环素耐药基因tetA、tetB、tetC、tetD、tetE、tetG及tetK，并对扩增产物进行测序。

The clenbuterol and ractopamine of specimen was extracted by 0.5% tungstenic acid: methanol (80:20). It was then purified by Oasis MCX. The mobile phase was 4 mmol/L ammonium acetate: acetonitrile (80:20). PDA detector was used for separation and detection.

采用0.5%钨酸：甲醉(80：20)提取试样中的盐酸克伦特罗和莱克多巴胺，用Oasis MCX小柱净化，4mmol/L乙酸胺溶液：乙腈(80：20)为流动相，用PDA检测器分离测定。

Except as described in 12.4.1.3, an insulated conductor of one circuit shall be separated or segregated from any uninsulated live part of a different circuit.

12.4.1.1除非提出了额定的最高绝缘电压，电路中绝缘导体同电源的分断用挡板断开或分离，除了12.4.1.3中所述的情况外，电路的绝缘导体应与不同电路的活动导体分离或断开。

The selected process consists of several steps as follows. Firstly, ammonifying the oily mixture of PXA pyrolysis by ammonia or aqueous ammonia, then adding the organic solvent to extra

实验结果表明，合适的分离提纯工艺为：先将裂解产物用氨水氨化、有机溶剂萃取，然后用硫酸酸化11-CUA铵盐水溶液、再将粗品11-CUA于合适介质中结晶，得到高纯度11-CUA晶体；优化工艺条件为：氨化pH值8.0～10.0、温度25-35℃，甲苯为萃取剂，酸化pH值1.5～2.0、温度55-60℃，环己烷为结晶介质，粗品11-CUA在环己烷中溶解温度50～55℃、与环己烷初始质量比4：100、结晶前沉降分层时间10min、结晶温度20℃。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。