分离用的

Methods The lymphocytes of rats were isolated from the peripheral blood and cultured with sodium arsenite under 37 ℃.The ROS content was detected by DCFH-DA;The content of LPO was detected by fluoresce method.

体外分离大鼠外周血淋巴细胞后，施加处理因素，在37℃条件下恒温培养，用2'7'-二乙酰二氯荧光素染色法检测细胞内的ROS水平，用硫代巴比妥酸荧光法测定细胞内LPO含量。

The sediment of sulfide can be separated from the solution by filtration and syphon. After the sediment is dissolved by diluted nitric acid, Ni 2+ and Co 2+ in solution can be determined by the flame atomic absorption spectrometry method.

将沉淀过滤，或者加入明矾以加速硫化物的沉降、凝结，再用虹吸法吸去上层清液的方法将沉淀分离，用热稀硝酸将沉淀溶解后直接用火焰原子吸收法测定镍、钴的含量。

Methods: The sample was absorbed with glass fiber filters paper and was desorpted by toluol. Trinitrotoluene was analyzed by capillary gas chromatography method with ECD detector.

用玻璃纤维滤纸采集空气中的三硝基甲苯，用溶剂洗脱后，经色谱柱分离，电子捕获检测器检测。

Methods : We subjected C57BL/6J mice to CS (whole body exposure, 90 min/day) for various periods, and used laser capture microdissection to isolate bronchiolar epithelial cells for analysis of mRNA by quantitative reverse transcription-polymerase chain reaction.

研究方法：研究者将 C57BL/6J 小鼠分别在不同时间周期暴露于CS（全身暴露，90分钟/天），用激光捕获显微切割技术分离细支气管上皮细胞，然后用定量逆转录多聚酶链反应分析上皮细胞的mRNA。

Methods Using petroleum ether to extract crude fat from the Capparis spinosa fruit and identify separately with GC-MS method after methanolysis.

方法］用石油醚提取刺山柑果实中的粗脂肪，甲酯化后，用GC-MS法进行分离鉴定。

This experiment with dilute alkali swelling on collagen extraction and separation, then purified by gel from collagen, and then glutaraldehyde, carbodiimide, has three types of di-isocyanate cross-linking agent on the plastic recombinant protein molecules cross-linked, the final test of infrared spectroscopy analysis of the results of their cross-linking to determine the extent of the effectiveness of cross-linking cross-linking agent to have an initial understanding.

本实验用稀碱溶胀法对胶原蛋白进行提取与分离，接下来用凝胶层析纯化所得胶原蛋白，然后用戊二醛、碳化二亚胺、己二异氰酸酯三种交联剂对胶原蛋白分子进行重组交联，最后通过对红外光谱测试结果的分析，对其交联程度进行判断，对交联剂的交联功效有一个初步的了解。，谢谢。

This paper studied the post-treatment process of polyvinylidene fluoride emulsion consisting of coagulation, washing-up, centrifugalization and drying procedure.

结果表明，用凝聚剂破乳、离心分离的PVDF乳液后处理工艺是可行的，凝聚效果明显，凝集粒径达到了28—32μm；离心分离完全，洗涤母液的电导率小于5μS/cm，制得的PVDF制品色泽纯白。

Myocardial SR and NE were fractionated with velocity and isopycnic gradient centrifugation.

用差速离心和密度梯度离心法分离心肌 SR和 NE，用同位素标记配体研究 Ry R的动力学特征。

Methods Compounds were isolated and purified by various column chromatographic techniques and their structures were elucidated on the basis of spectral analysis (IR, ESIMS, 1HNMR, 13CNMR). The inhibitory activity against the proliferation of HL60, REH and Raji cell lines was determined by using MTT assay.

用色谱方法分离栀子中化学成分，用波谱技术(IR，ESIMS，1HNMR，13CNMR)进行结构鉴定，用四唑盐比色法测定化合物对白血病细胞（HL60人早幼粒细胞白血病、REH人急性淋巴细胞白血病、 Raji淋巴瘤）增殖的影响。

METHODS: Separating the constituents of Schisandra chinensis using various kinds of chromatography and identifying their structures on the basis of spectral analysis.

用各种色谱方法分离北五味子的化学成分，用波谱技术鉴定结构。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。