分离用的

Methods Human pancreas tissue was digested with collagenase P using a semi-automated pancreas-digestion system followed by purification in a HCA-Ficoll continuous gradient using Cobe2991 cell separator.

方法使用自制的半自动人胰腺消化分离装置及胶原酶P进行人胰腺的消化分离，用COBE2991细胞分离机及HCA-Ficoll纯化人胰岛细胞。

Methods SH-SY5Y cells, a human neuroblastoma cell line, were incubated with different concentrations of fluoride for 48 hr and somle of them were treated with vitamin E precedently. The functional situation of cells was measured by MTT method; lipid peroxidation was detected by High-Performance Liquid Chromatography; phospholipid was separated by a Silica SepPak cartridge and neutral lipids by HPLC.

体外培养SH-SY5Y人脑神经母细胞瘤细胞，在培养液中加入不同浓度的氟化物或加入抗氧化剂，培养48h后用测定细胞MTT的方法来了解细胞的损伤程度，用高效液相色谱法分离和测定培养液中脂质过氧化物水平，用过柱和比色法测定细胞生物膜磷脂含量，用高效液相色谱法测定细胞生物膜辅酶Q和胆固醇含量。

Isolation purification of NRCs:The NRCs were collected from the heart of neonatal rats,they were digested with steapsin and different speed of adhesive-screeing .

NRCs的分离、纯化：取出生1天的SD乳鼠，用胰酶消化和差速贴壁方法分离、纯化NRCs。

The separation of optical isomers of 2, 2'-dihydroxy-1, 1'-binaphthyl and 2, 2'-diamino-1, 1'-binaphthyl was studied by reversed-phase high performance liquid chromatography on a chiral stationary phase consisting of-hydroxypropyl-β-cyclodextrin (Cyclobond Ⅰ 2000 SP)using methanol/water as the mobile phase. The effects of mobile phase ratio, flow rate and column temperature on separation of optical isomers of DHBN and DABN were investigated.

在S-羟丙基－β-环糊精手性柱(CyclobondⅠ2000 SP)上用反相高效液相色谱法研究了2，2'-二氨基－1，1'-联萘和2，2'-二羟基－1，1'-联萘光学异构体的分离，考察了流动相组成、流速以及柱温等对联二萘胺和联二萘酚光学异构体分离的影响。

Traditional separation method: Several years ago, as a result of the equipment condition limit, the material particle DNA separation with the CsCl balanced equilibrium density centrifuge process, the idiomorphic become generally the gradient.

传统的分离方法：数年前，由于受设备条件限制，质粒DNA的分离一般用CsCl平衡等密度离心法，自形成梯度。

Methods Human umbilical cord blood samples were obtained sterilely from healthy parturients. MSCs were isolated by lymphocyte separation medium (density 1.077g/ml), MSCs were obtained by adhere culture. The surface antigen expression of MSCs was detected by flow cytometry.

将无菌条件下采集健康产妇脐带血，用相对密度为1.077g/ml的人淋巴细胞分离液分离脐血单个核细胞，利用间充质干细胞贴壁生长的特性获得MSCs，流式细胞仪检测其表面抗原标志。

In chapter 3, 83 volatile chemical constituents have been identified from the petroleum ether extracted part of ethanol extract of Debregeasia longifolia Wedd., and the relative percentages of the compounds are determined by GC method. From the extract of trichloromethane and ethyl acetate, 8 compounds have been isolated.

第三章首次对长叶水麻乙醇提取物的石油醚萃取部分分离并鉴定出83种成分，并用毛细管气相色谱面积归一化法测定了各组分的相对百分含量；从氯仿和乙酸乙酯萃取部分分离得到了8种化合物，用波谱学方法鉴定了其中2种成分。

The results showed: after local leafhoppers were inoculated with RDV- SX isolate, RDV- LQ isolate, the vector viruliferous rates were similar as 84 % and 75% respectively,but the rice incidence rates were dissimilar as 28.1% and 2.8 % respectively.

研究表明：用本地黑尾叶蝉分别接种RDV本地分离物和云南分离物，斑点杂交显示介体带毒率相似，分别为84%、75%，但生物学接种结果差异相当大，分别为28.1%，3.8%，另外斑点杂交显示云南病区的黑尾叶蝉带毒率为88%，说明介体叶蝉的传毒能力具有地域性，介体叶蝉带毒率与传毒能力也存在一定差异。

Albicans strains with fluconazole. Method: A series of 16 strains C. albicans from the same body were induced to form pseudo-hyphae in RPMI 1640 medium and DMEM medium at 37 癈 for twenty-four hours respectively. The pseudo-hyphae and yeast were counted and the ratio between pseudo-hypha and total cells was calculated. C. albicans strains were induced to form pseudo-hyphae in RPMI 1640 medium for seven days by transferring twelve times. The genome DNAs of the hyphal-form of C. albicans was cleaved by two restriction enzymes EcoR I and Bgl II, the products were electrophored and photoed.

以同一母体来源的白念珠菌CA-1～A-17(共16株)作为研究对象，采用RPMI1640和DMEM培养基，37℃培养24h诱导其假菌丝形成，记录不同观察时间时的菌丝相和酵母相细胞数，计算菌丝相白念珠菌形成率；以RPMI1640培养基，37℃连续传代培养7天(转种12次)后，分离菌丝相白念珠菌并提取其基因组DNA；以EcoRⅠ和BglⅡ消化菌丝相基因组DNA，消化产物进行琼脂糖凝胶电泳并拍照；用随机引物(5'…TCACCACGGT…3')扩增菌丝相基因组DNA，采用变性聚丙烯酰胺凝胶电泳分离扩增产物并拍照。

Then they were stocked by three preservation methods using slant culture, liquid paraffin and liquid nitrogen, respectively.

分离纯化了形态差异较大的21株座壳孢，用斜面低温、液体石蜡和液氮保存这三种方法对分离的菌株进行了保藏。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。