分离用的

Then the intracellular calcium concentration of neurons was measured by laser confocal scanning microscope , represented by the mean intracellular fluorescent intensity. On the ice, the cerebral cortical tissue at the infarction margin of the section after optic chiasm was rapidly dissociated about 100mg, then made into brain homogenate in low temperature. The high performance liquid chromatography was used to determine the Glu, Asp and Gly contents in homogenized brain tissue.

视交叉平面用2％TTC染色，用图像处理软件计算出脑梗死面积占该片脑组织面积的百分比；取视交叉前平面梗死边缘区皮层组织约100mg，用酶消化法制作神经细胞悬液，用激光扫描共聚焦显微镜检测皮层神经元细胞内游离钙离子浓渡，以细胞平均的荧光强度代表细胞内钙浓度；冰上迅速分离视交叉后梗死边缘区皮层组织约100mg，于低温下制作脑组织匀浆，用高效液相色谱法测定皮层匀浆中谷氨酸、天门冬氨酸、甘氨酸的含量。

First 27 rabbits of the experimental group were destabilizated by operated on L6、7.Inj Diazepam 0.25 mg/kg,Inj Ketamine 0.02 g/kg and Inj Atropine 0.125 mg/kg was one by one injected into the rabbit through the auris vein,shearing the rabbit hair of backside waist,fixing the rabbit on operation table in face lying,using 1% Povidone Ioding to degerm the operation area.Every rabbit was incised at the backside of its waist,that incisal opening is located in the center of the intervertebral space (L6、7 space) that both side iliac crest line correspond ,from the meso-ordinate direction cut about 4 cm incision,cutting open skin and subcutaneous tissue,thoroughly ,exposing the spinous processes the vertebral plates and the upper-inferior articular processes,entirely segregating the muscles that cohere the spinous processes the vertebral plates and the articluar process,then excising the supraspinal ligaments and interspinal ligaments,biting off two sides the inferior articular processes of L6,in order that resulting in intervertebral destabilization,using 0.9% Inj.Sodium Chloride to washout the incisal opening,in order sewing up each layer tissues.

方法］ 取48只6个月龄日本大耳白兔，雌雄不限，体重为（2.5±0.2） kg，随机进行分组，分为对照组和实验组，对照组为21只；实验组为27只；先将实验组兔腰背部皮毛剪除，用安定注射液1.25 mg/kg、氯胺酮0.02 g/kg、阿托品0.125 mg/kg顺次耳缘静脉注射麻醉后，俯卧固定于手术台上，用1%碘伏消毒手术区域，以髂嵴平对椎间隙（即L6、7）为中心，从正中取一长约4 cm纵行切口，切开皮肤及皮下组织，锐性分离，暴露棘突、椎板及上下关节突，将附着于棘突、椎板及小关节的肌肉全部分离开，然后依次切除L6、7棘上及棘间韧带，咬除第6腰椎两侧下关节突，造成椎间失稳，用无菌生理盐水冲洗切口，依次缝合各层组织；术后动物在笼中自由活动。

Inj Diazepam 0.25 mg/kg,Inj Ketamine 0.02 g/kg and Inj Atropine 0.125 mg/kg was one by one injected into the rabbit through the auris vein,shearing the rabbit hair of backside waist,fixing the rabbit on operation table in face lying,using 1% Povidone Ioding to degerm the operation area.Every rabbit was incised at the backside of its waist,that incisal opening is located in the center of the intervertebral space (L6、7 space) that both side iliac crest line correspond ,from the meso-ordinate direction cut about 4 cm incision,cutting open skin and subcutaneous tissue,thoroughly ,exposing the spinous processes the vertebral plates and the upper-inferior articular processes,entirely segregating the muscles that cohere the spinous processes the vertebral plates and the articluar process,then excising the supraspinal ligaments and interspinal ligaments,biting off two sides the inferior articular processes of L6,in order that resulting in intervertebral destabilization,using 0.9% Inj.Sodium Chloride to washout the incisal opening,in order sewing up each layer tissues.

方法］ 取48只6个月龄日本大耳白兔，雌雄不限，体重为（2.5±0.2） kg，随机进行分组，分为对照组和实验组，对照组为21只；实验组为27只；先将实验组兔腰背部皮毛剪除，用安定注射液1.25 mg/kg、氯胺酮0.02 g/kg、阿托品0.125 mg/kg顺次耳缘静脉注射####后，俯卧固定于手术台上，用1%碘伏消毒手术区域，以髂嵴平对椎间隙（即L6、7）为中心，从正中取一长约4 cm纵行切口，切开皮肤及皮下组织，锐性分离，暴露棘突、椎板及上下关节突，将附着于棘突、椎板及小关节的肌肉全部分离开，然后依次切除L6、7棘上及棘间韧带，咬除第6腰椎两侧下关节突，造成椎间失稳，用无菌生理盐水冲洗切口，依次缝合各层组织；术后动物在笼中自由活动。

In continuing efforts to identify active components from traditional Chinese herbal medicine, professor Yao and co-worker also obtained this compound from the rhizome of Dioscoreaceae by repeated bioactivity-guided isolation and tested its anticancer activities in vitro with a panel of 60 human cancer cell lines performed in NCI.

长期以来，姚新生教授等一直致力于从传统中草药中寻找活性成分的研究，他们采取活性追踪分离方法，从粉荜解中同样分离得到了methyl protodioscin并经NCI用60种人癌症细胞株筛选其体外抗癌活性。

The results show that jamesonite and pyrrhotite have good floatability in the pH range of 2-10 and marmatite has good floatability in the acid and it is difficult to separate these minerals. In the pH range of 6-8, the separation of jamesonite and pyrrhotite, marmatite is possible when using mercaptoacetic acid and mercaptoethanol as depressants. In case of the presence of Cu2+ ion, however, it is difficult to separate the three minerals. Infrared spectrum demonstrates that there are a number of function groups such as —SH,—COOH,—OH in the molecular structure of depressant.

结果表明：用丁黄药作捕收剂，脆硫锑铅矿和磁黄铁矿在pH 2~10范围内都有良好的可浮性，铁闪锌矿在酸性条件下可浮性好，三者分离困难；在pH 6~8之间，采用巯基乙酸和巯基乙醇作抑制剂，脆硫锑铅矿与磁黄铁矿和铁闪锌矿的分离是可能的；但铜离子存在时， 3种矿物之间的选择性分离是难以实现的。

The subendocardial and sub-epicardial tissues were separated from the mid-layer region with opthalmic scissors. Single cells in each region were obtained by gentle shaking and dispersing in a chamber filled with normal Tyrode's solution. The whole-cell patch clamp technique was used to record the current while change the composition of the perfusate.

采用胶原酶二步消化法分离兔心肌细胞，用锐利眼科剪分离左室游离壁内、中、外三层心肌，改变灌流液的成份，采用全细胞膜片钳记录离子电流。

Respectively, glutamic acid derivatized β-CD has been synthesized with epoxy chloropropane as the intermediate, a water-insoluble yellow liquid was got after the acetylating of the by using acetic anhydride, which was used as a novel gas chromatographic CSP; A water-soluble β-CD polymer cross-linked with epoxy chloropropane has been synthesized, and the derivatives of β-CD polymer were synthesized by its alkylating and acetylating. The different β-CD derivatives were coated on silica capillary columns by dynamic method, the chromatographic properties and retention behaviors of the fused silica capillary columns were studied, the results showed that the β-CD derivatives had good stereoselectivity and separation results to some compounds, especially to various positional isomers and enantiomers.

本文还分别以环氧氯丙烷为中间体，合成了七{2，6-二-O-[3-(1，3-二羟基丙氨酸)]}-β-环糊精，再用醋酸酐将其乙酰化，得到了一种淡黄色粘稠液体，合成了一种新型的环糊精衍生物；以环氧氯丙烷作交联剂合成了一种水溶性的β-环糊精聚合物，再将其烷基化和酰基化，合成了β-环糊精聚合物的不同衍生物，用动态法将不同类型的β-环糊精衍生物涂渍在石英毛细管柱上，通过对所制备柱柱性能的测试和对一系列化合物的分离和保留行为的研究，结果表明所合成的β-环糊精衍生物对各类化合物，特别是对位置异构体和对映异构体具有较好的分离效果，显示了很强的立体选择性。

Further, based on the improved shiftwork targeting method, integrated exergy grand composite curve of distillation column and the top-level analysis approach to low-temperature process system are combined and extend to form the overall energy integration strategy of the low-temperature separation process.

面向低温分离过程系统的能量优化综合问题，基于复杂蒸馏塔的用能剖面分析法，提出了蒸馏塔集成的有效能总组合曲线，用以指导蒸馏塔的集成：并以此为核心工具，结合改进轴功目标法，发展了低温分离过程系统的整体能量集成策略，用于指导以减少冷冻系统轴功为目标的低温分离过程系统与低温背景过程的整体集成。

This study concerns on a new technology on preparation of alkyd industrial primer paint with tall oil obtained from waste fluid in papermaking.

研究用简单分离工艺从造纸废液中分离塔尔油制备工业底漆的新工艺。

Results: The raio of cell seperation is more than 95% by using Selfmade magneticmicrospheres.

结果：用直接包被法制备的免疫磁珠分离CD4+T淋巴细胞亚群，分离率大于95％。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。