分离用的

Actomyosin were purified from human heart, and further isolated into CMLC with urea degradation and ammonium sulfate precipitation, CMLCⅠ and CMLCⅡ were purified later from this CMLC by the improved PAGE method, both CMLCⅠ and CMLCⅡ were without any protein contamination.

从人心肌提取肌动球蛋白，用尿素降解法和硫酸铵沉淀法从肌动球蛋白中分离、纯化CMLC，应用改进的聚丙烯酰胺凝胶电泳法从总CMLC中分离CMLCⅠ和CMLC Ⅱ，二者均不含有任何杂蛋白。

In this paper, a method of analysis which was based on thin layer chromatography was introduced. Benzimidazole derivative and amine were separated at first.

文中采用薄层层析法分离苯并咪唑衍生物及杂质，首先分离出苯并咪唑衍生物，然后用非水滴定方法分析含量，得到了准确的分析结果。

In this paper, the recent development of high-speed countercurrent chromatography applied in the fields, including the selection of solvent system, the separation of active ingredient and the method of separation, are reviewed.

文中对高速逆流色谱用於分离中药有效成分的溶剂系统选择、采用该方法分离的中药有效成分以及分离手段等方面的研究状况进行了综述，并展望了该领域的研究前景。

In this dissertation, firstly, the conditions of isolation and purification of specific toxin fractions produced by Exserohilum turcicum has been studied, and a high-efficient method has been established;secondly, the toxins were analysed by UV-Vis spectrophotometry;then the effect of specific toxin on death rate of corn leaves protoplast was studied by FDA as a tinct reagent;finally, using ANS as a probe and SDS-PAGE to study the membrane protein of the corn leaves with Htl gene. In a word, the aim of the research is to search some existent evidences of the toxin binding site on protoplasmic membrane, and provide proofs for nosogenesis of specific toxin in terms of cytology and the molecular biology.

本研究对玉米大斑病菌1号小种毒素的特异性组分进行分离，建立了高效的特异性毒素分离纯化方法，并对其进行了紫外波谱分析；用荧光素双醋酸酯染色法，测定了毒素特异性组分对原生质体死亡率的影响；进而用1-苯胺基-8-萘磺酸荧光探针标记法和SDS-PAGE电泳法对特异性毒素组分与Htl基因玉米细胞互作过程中的质膜蛋白进行了研究，旨在证明Htl基因玉米细胞质膜上存在特异性毒素组分结合蛋白，为从细胞和分子生物学角度阐明毒素的致病机理提供证据。

In this paper, we studied the separative effect of protein components in crude Morinda polysaccharide by the Sevag method in order to compareison through Kjeldahl Determination of protein content change before and after separation, and to anthrone-sulfuric acid measured the content changes polysaccharide content before and after separation.

本文通过以凯氏定氮法测定巴戟天粗多糖经Sevag法分离前后蛋白质含量的变化，并以蒽酮一硫酸法测定分离前后多糖含量的变化比较，研究了用Sevag法对巴戟天粗多糖中的蛋白质成分分离的效果。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum.

为了比较大鼠胎血和骨髓中非造血成体干细胞（non-hematopoietic adult stem cells，NASC）体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同，在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓，用Ficoll-hypague分离液分离出单个核细胞后，种植于含10%胎牛血清的DMEM/LG培养液内。收获的NASC传代培养，用流式细胞仪检测细胞的免疫表型。

The authors carried many researches of the feasibility about enriching and separating trace zinc from almost cadmium, and the rate of recoveries of several common metal ions according to this methodo Through repeatedly probed and tried out, at last, a thought as collaborated with traditional methods was established - using diantipyrinylmethane as complex reagent ,with ammonium thiocyanate and zinc forming the triple ionic compound in the medium of hydrochloric acid , extracted by trichloromethane, extracted the organic system by ammonium hydroxide in order to gain zinc .

本文主要研究了从镉基体中富集分离微量锌的可行性，以及采用该方法几种常见金属离子的回收率。通过反复的摸索、试验，最终确立了与传统方法相结合的思路—采用二安替比林甲烷作为络合剂和硫氰酸铵、锌在盐酸介质中形成三元离子缔合物，用三氯甲烷作为萃取剂，最后用氨水作为反萃剂将有机相中的锌洗脱出来与镉分离。

The effect of bacteriostatic such as penicillin, norfloxacin, nalidixic acid, potassium dichromate and tatin were studied individually. The orthogonal design was used in this experiment and the software STATISTICA6.0 was employed to analyse the result. The result showed that the potassium dichromate was the best bacteriostatic among those bacteriostatices.

研究了青霉素、萘啶酸、制霉菌素、重铬酸钾和氟哌酸5种抑制剂对分离土壤放线菌的影响，其中以重铬酸钾的分离效果最好，其次为制霉菌素；通过正交试验研究了重铬酸钾、萘啶酸、制霉菌素3因子组合对放线菌分离的影响；最后用STATISTICA6.0进行分析。

Methods: A randomized, single blind controlled clinical design was used to accomplish the series clinical trials included 123 cases. The troop of gatifloxation injection with sodium chloride included 20 cases in gatifloxation group and 23 cases in ciprofloxation group; The troop of gatifloxation injection with glucose included 21 cases in gatifloxation group and 19 cases in levofloxacin group; The troop of gatifloxation pivoxil included 19 cases in gatifloxation group and 21 cases in ciprofloxation group. The antibacterial activity of clinical isolates were detected by scrips which contain gatifloxation(5ug/L), levofloxacin (5ug/L), ciprofloxation (5ug/L), spafloxation(5ug/L), cefotaxime(30ug/L), penicillin(10U/L) respectively. The MICs of sixkinds of antibiotics were detected by 2-fold agar dilution method. Result: The common data of patients in the two groups of 3 troops were similiar.

采用区组分层均衡随机单盲试验设计，分别完成甲磺酸加替沙星氯化钠注射液组试验药20例及对照药23例、甲磺酸加替沙星葡萄糖注射液组试验药21例及对照药19例、甲磺酸加替沙星片剂组试验药19例及对照药21例共123例感染患者的临床试验；用K-B法测定临床分离致病菌对加替沙星(5ug／L)、左氧氟沙星(5ug／L)、环丙沙星(5ug/L)、司帕沙星(5ug/L)、头孢噻肟(30ug／L)、青霉素(10U／L)纸片的敏感性，再用按美国国家实验室标准委员会(National Committee for Clinical Laboratory Standards，NCCLS)推荐的琼脂平皿对倍稀释法测定临床分离菌株对加替沙星、左氧氟沙星、环丙沙星、司帕沙星、头孢噻肟、青霉素的最低抑菌浓度(Minimum Inhibitory Concentration，MIC)以了解其体外抗菌活性。

Methods Four pretreatments and 5 media supplemented with different inhibitors were used for isolation.

用不同的样品预处理方式和分离培养基，并添加不同的抑制剂进行分离；根据放线菌的菌落数和出菌率确定最佳分离方法组合。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。