分离用的

Methods : The neural retina was isolated from RPE by enzyme one containing 220u/ml hyaluronidase and 0.1% trypsin, later the RPE cells was isolated from the Bruch"s membrane by enzyme two containing 0.25% trypsin, then the resembled RPE cells was injected into the host"s subretinal space with a special equipment after producing an artificial bleb detachment of neural retina.

用酶Ⅰ(含220u/ml透明质酸酶和0.1％胰蛋白酶)分离视网膜神经上皮层与RPE之间的联系，再用酶Ⅱ(含0.25％胰蛋白酶)将RPE从玻璃膜上分离下来获取悬浮的RPE细胞，用特制的移植针头通过人为造成的局限性视网膜脱离区将其移植入受体视网膜下间隙。

The company mainly produces air breathing apparatus, positive pressure air respirators, emergency escape breathing apparatus devices | EEBD, marine oil-water separators | Marine oil-water separator devices | Marine Bilge oil water separator devices, marine fire equipment | fire equipment, positive pressure breathing apparatus air cylinders standby, marine fire extinguisher, PQ8.C foam-type portable air gun device, the international shore connection, fire insulation clothing | clothing insulation, fire anti-chemical suits, many with water cannon, fire-resistant rope | refractory lifeline, explosion-proof lights, the release of hydrostatic pressure, and heat appliances, life jackets, life-saving thermal insulation suits, life buoy, life jacket lights, life buoy lights, liferaft lights, life raft racks, marine oil-water separator devices, marine red flame signal, Marine orange smoke signal, lifebuoy from the bright lights floating smoke signal, lifebuoy with orange smoke signal, Marine line-throwing equipment, marine life-saving throw rope, and marine oil-water separator devices, etc. Products.

公司主要生产的空气呼吸器，正压式空气呼吸器，紧急逃生呼吸器装置|EEBD，船用油水分离器|船用油水分离器装置|船用舱底油污水分离器装置，船用消防员装备|消防员装备，正压式空气呼吸器备用气瓶，船用灭火器，PQ8.C型手提式空气泡沫枪装置，国际通岸接头，消防隔热服|隔热服，消防防化服，多用水枪，耐火绳|耐火救生绳，防爆灯，静水压力释放器，保温用具，救生衣，保温救生服，救生圈，救生衣灯，救生圈灯，救生筏灯，救生筏架，船用油水分离器装置，船用红色火焰信号，船用橙色烟雾信号，救生圈自亮浮灯烟雾信号，救生圈用橙色烟雾信号，船用抛绳设备，船用救生抛绳器，船用油水分离器装置等产品。

It is more practical to obtain one uranium product and one uraniumplutonium mixture product through partial separation of uranium and plutonium.

系统分析了用稀硝酸反萃分离萃入30％TBP／煤油中的铀钚的工艺，分析了各操作变量的影响，结果表明用稀硝酸反萃分离铀钚是可能的，铀钚部分分离得到有机相铀产品和水相铀钚混合物产品的工艺较为现实可行。

Recombinant plasmid pSVH 7 DNA of avian influenza virus H7 subtype heamagglutinin gene was encapsulated with DC-chol/DOPE liposomes and PC/chol/SA liposomes separately. Two-week old SPF chickens were intramuscularly inoculated with 50 μ g/0.2ml of the liposome entrapped PSVH 7 DNA. Four-weeks later, each chicken was challenged with 0.1ml 〓 AIV . One week after the challenge, the secretion of the cloacas was collected and transfected to chicken embryos to isolate the virus. The virus was isolated from 6/6 of the control group, 1/6 of the naked DNA group, 1/6 of the PC/chol/SA entrapped DNA group and 0/6 of the DC-chol/DOPE liposome entrapped group. The HI antibody titers (log2) of the four groups were 6. 83±0.98, 7. 0±1. 26, 7. 83±1. 17 and 8. 00±0.89 respectively 1-week after challenge, and 8. 5±0.55, 8. 17±0.82, 8. 68±0.45 and 9. 33±0.54 respectively 2-week after challenge. The results showed that inoculation of liposome entrapped DNA significantly enhanced resistance to virosis in animals.

将含禽流感病毒H7亚型血凝素基因的重组质粒pSVH7用DC-chol阳离子脂质体和胆固醇／卵磷脂／十八胺脂质体包裹，免疫2周龄SPF鸡，4周后用同型禽流感病毒进行人工感染，1周后采集泄殖腔分泌物分离病毒，结果未免疫组6／6分离到病毒，裸质粒DNA免疫组1／6分离到病毒胆固醇／卵磷脂／十八胺脂质体包裹DNA免疫组1／6分离到病毒，DC-chol脂质体DNA免疫组没有分离到病毒（0／6）：人工感染后1周各组的HI抗体（Log2）分别为6.38±0.98，7.00±1.26，7.83±1.17，8.00±0.89，2周后为8.50±0.55，8.67±0.82，8.68±0.45，9.33±0.52，脂质体包裹组在同期均高于未免疫组和裸DNA免疫组，表明脂质体包裹质粒DNA免疫动物后，能增加动物对病毒感染的抵抗力和反应能力。

Take the 10~12ml single-valve capacity as the example, with flings turns the head to separate evenly, 36.000rpm 60 the hour, turns the head with the angle type to separate 45, OOOrpm 36 the hour, the former including adds the deceleration to use in common 130,000,000 revolution of actuation department lives, the latter must use 100,000,000 revolution of actuation department lives, this was 100 ~ 20,000,000,000 transfers to at that time the supercentrifuge total life looked, without doubt each time tests the expense to be excessively high, in addition the CsCl amount used are many, price expensive and so on factors, causes this kind of separation purification work to become the very expensive experiment.

以10~12ml单管容量为例，用甩平转头分离，36.000rpm 60小时，用角式转头分离45，OOOrpm 36小时，前者包括加减速在内共用去1.3亿转驱动部寿命，后者也要用去1亿转驱动部寿命，这对当时超速离心机总寿命为100~200亿转来看，无疑每次实验费用过高，加上CsCl用量多、价格贵等因素，使这类分离纯化工作成为非常昂贵的实验。

Methods:Human embryonic tissues containing genital ridges and dorsal mesenteries were isolated and then cultured in 5 different patterns: simple mechanical disaggregation,trysinization, combination of the former 2 and with/without feeder cells.

体外分离人胚胎生殖嵴和肠系膜组织，按单纯机械分离、酶消化分离、二者结合法及是否有饲养层将组织分为 5种不同的方式培养后，用碱性磷酸酶标记，计数阳性克隆并进行比较。

In the energy-utilization analysis stage, based on the improved shiftwork targeting method, the top-level analysis of lowtemperature process system and the overall energy integration strategy of lowtemperature separation process are used to analyze, diagnose and improve the energy-utilization of low-temperature process system, specially the lowtemperature separation system.

在用能分析阶段，结合低温过程系统顶层分析方法和低温分离过程系统整体能量集成策略，以减少冷冻系统轴功为目标，并辅以经济性分析，侧重于低温分离过程系统的能量集成进行低温过程系统用能的分析、诊断和调优；在复合式方法的用能优化阶段，则根据用能分析阶段所得调优结果，采用分层—逐级式低温换热网络与冷冻系统集成优化方法，以换热网络与冷冻系统总费用最小为优化目标，对低温换热网络与冷冻系统进行集成优化。

The optimized conditions for isolation and culture of endophytic bacteria isolated from grasses in alpine area in this study. The different tissues of grasses were immersed in 0.1% of SDS for 15 min, 3% of NaClO for 3 min, 0.1% of mercuric chloride for 10 min, 75% of alcohol for 1 to 2 min. The endophytic bacteria were isolated after aseptic grinding and diluted to 10^(-3) and using TSA culture medium for 5 to 7 days under 28℃.

通过对高寒牧草内生细菌的分离培养方法研究，得出了优化的分离培养条件，即牧草不同组织器官用0.1%SDS浸泡15min、3%NaClO浸泡3min0.1%升汞浸泡10min、75%酒精1~2min处理后（各步间均用无菌水冲洗3~4次），研磨并稀释至10^(-3)，涂布于TSA培养基上，置28℃恒温条件下培养5-7d，可从高寒牧草组织中分离获得数量、种类较多的内生细菌。

Unscrew the locking nuts on the release lever, meanwhile unscrew the adjusting nuts using a spanner, while doing that measure the gaps between the release levers and bearings by means of feeler gauge, until the gap reads 0.3-0.5mm. Repeat it for three release levers, their gaps should be the same, after that screw up the locking nuts, and check it to make sure.

b、旋松分离杠杆上的锁紧螺母，一面用扳手转动调节螺母，一面用厚薄规测量分离杠杆与分离轴承之间的间隙，直到间隙为0.3-0.5毫米为止，三只分离杠杆依次都调整好，再将锁紧螺母锁紧，然后复查一遍。

B. Unscrew the locking nuts on the release lever, meanwhile unscrew the adjusting nuts using a spanner, while doing that measure the gaps between the release levers and bearings by means of feeler gauge, until the gap reads 0.3-0.5mm.

b、旋松分离杠杆上的锁紧螺母，一面用扳手转动调节螺母，一面用厚薄规测量分离杠杆与分离轴承之间的间隙，直到间隙为0.3-0.5毫米为止，三只分离杠杆依次都调整好，再将锁紧螺母锁紧，然后复查一遍

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。