分离用的

Simplier and more efficient ion extraction methods for atomic vapor laser isotope separation AVLIS were evaluated using a 2-D electron-equilibrium model to compute and compare three electrostatic methods the parallel electrode method, the Π type electrode method, and the improved M type electrode method.

为探索用于原子蒸气激光同位素分离AVLIS的简单有效的离子引出方法，用二维流体理论电子平衡模型计算比较了3种静电场方法平行板电场法，Π型电极法，改进M型电极法计算发现，二维流体理论电子平衡模型并不能很好地描述Π型电极法对另外两种静电场方法，计算了不同外加电压、不同的初始原子密度和不同的目标同位素丰度等条件下的离子引出情况从计算结果可以看出，改进M型电极法不论是从离子引出时间还是从碰撞损失率，都要比平行板电极法好得多当外加电压较高或初始原子密度较高时，这种好处就更加明显因此，改进M型电极法是一种可供实际采用的比较好的静电场方法

We would like to make our students to know and master the basic way of the chemistry experiments, the use of the normal apparatus of inorganic and analytical chemistry, the character of the normal inorganic compounds and

课程的目标是使学生了解和掌握化学实验的基本方法和常见的无机与分析化学仪器的使用，了解和掌握一般无机化合物的性质以及分离提纯的方法；熟悉常见的无机化合物的制备方法，掌握测定有关重要化学常数的方法和原理；熟悉滴定分析的基本操作，掌握定量测定无机化合物的主要化学滴定的方法和原理，从而培养学生能用化学观点与方法来观察分析和解决实际问题的能力。

We used Aeromonas hydrophila which we separated from the diseased Paralichthys olivaceus to made a inactivated whole cell bacterin .We injected this vaccine in the mice and the Paralichthys olivaceus .Both the mice and the Paralichthys olivaceus gained a good protection of 80% and 70%.The fish with dipping immersion gained a good protection of 85%,and the incidence of disease was 6%.We detected the antibody level in the antiserum by agglutination reaction and indirect ELISA which seemed to be immunogenic crucially.We anatomized the mice then we found that all the purtenance of the immunzied mice are healty.All of this proved that the vaccine is effective.

本实验将所分离到的嗜水气单胞菌用终浓度为0.2%~0.5%的甲醛对菌体进行灭活，制备了灭活疫苗，并优化灭活疫苗的灭活条件；将制备的灭活疫苗分别注射小鼠和牙鲆，均取得了较为理想的效果，其中小鼠的免疫保护率达到80%，牙鲆的免疫保护率达到70%；采用渗透压差法大规模浸泡免疫牙鲆也取得了很好的效果，浸泡免疫过的牙鲆发病率仅为6%，攻毒后免疫保护率为85%；通过应用间接ELISA方法检测受免动物的抗血清，并将牙鲆的抗血清注射新西兰白兔，从而获得兔抗鱼血清，更便于检测，结果都表明动物体内产生了较高的抗体水平；剖检攻毒后的动物，发现免疫过的动物内脏健康无异样，表明该疫苗安全有效，从而为防治牙鲆腹水症开辟了一条新途径，也为进一步制备其基因工程疫苗奠定了基础。

The motionless backgroup situation usually happens at the video camera parked to opposite quiescent condition , the first wipe off yawp by using pretreatment method,the second using the method of difference Statistic in two frame to analyse and compute background , Above this base to utilize the resumeed background and present frame message detect movement target and it"s movement track ; In dynamic backgroup movement image,it is very difficult to exactly compute ground since the background is varied complexly , the article research the optical flow model、 background model、 moving object detection based on entropy,and depart target from background in movement picture , detect moving target and plain it"s track.

静止背景的情况通常发生在摄像机相对静止状态，首先用预处理的方法去除噪声，其次采用帧间差分统计的方法进行分析计算背景，在此基础之上利用恢复的背景及当前帧的信息检测运动目标及其运动轨迹；对于动态背景的运动图像，由于背景是不断变化且呈现出复杂的特征，因此很难准确求出静止背景，本文在光流矢量场和配准方法的基础上探讨研究了光流模型、背景模型和基于熵的方法，在动态图像上分离不同的目标与背景，直接检测出运动目标并画出相应的轨迹。针对运动图像的复杂性，本文提出了构建模型知识库的设想。

And a Bottle of Rum tells the raucously entertaining story of America as seen through the bottom of a drinking glass.

就象是从一瓶装满水的瓶子底部往上看一样，《一瓶朗姆酒的故事》从一个独特的视角告诉了我们美国历史上所发生的一些有趣故事，这本书对每一种鸡尾酒都用了专门的一章来叙述-从公海上酗酒的船员到酒吧里跳舞的现代青年，威尼克提斯向我们揭示了本土文化，这种文化是从曾迅速发展的糖类贸易而产生的工业废弃物中分离出来的，已经成功渗透到新世界的每一个角落。

The cells were cultured under the following serum microenvironment. The primary cells of autoserum group were cultured with autoserum, changing the medium with fetal bovine serum after passage. The primary cells of homogeneity foreign serum group were cultured with homogeneity foreign serum, changing the medium with fetal bovine serum after passage. The primary cells of fetal bovine serum group were cultured with fetal bovine serum, and cultured with fetal bovine serum after passage. The primary cells of Dulbecco's modified Eagle's medium group were cultured with serum-free DMEM, changing the medium with fetal bovine serum after passage.

分别以下列血清微环境培养：自身血清组：分离细胞后原代用含大鼠自身血清的培养基培养，传代后换用含胎牛血清的培养基培养；同种异体血清组：分离细胞后原代用含同种异体血清的培养基培养，以后用胎牛血清培养基培养；胎牛血清组：分离后用含胎牛血清培养基培养，以后一直用含胎牛血清培养基培养；DMEM组：分离细胞后原代用不含血清的DMEM培养基培养，传代后换用含胎牛血清的培养基培养。

The soil and bark samples collected from Yunnan, Hebei and Shanxi provinces of China, and also from Vietnam, were used as the materials in this experiment. And dung pellets of rabbits or goats that had been autoclaved can be used to induce many kinds of fruiting bodies of the Myxobacteria in different colors, sizes, and shapes. We isolated and purified Myxobacteria strains with the improved method which was found by our laboratory.

本实验以采集自越南以及我国的云南、河北、陕西各地的土样和各种树皮、腐木作为实验材料，利用兔、羊粪球和滤纸作为基质诱导出多种颜色丰富、形态多样的粘细菌子实体，采用本实验室摸索出的较为有效的纯化粘细菌的方法，在实验器材上用24孔细胞培养板代替传统的平皿，有效地缩短了粘细菌的纯化周期，提高了分离纯化的效率。

DHCC Constance in the chemistry belongs to the onlighted Cyclopentanoperhydrophenanthrene when it reacted together with the acid Constance it could transform into the fluorescence phenol.we did the flouric determination for the DHCC, in which treated by the ethanol-sulfuric acid or acetic anhydride-methylene chloride-sulfuric acid, acquired standard curve in the 0-40μg/ml presented the liner relet...

DHCC类物质在化学上属于不发光的环戎烷多氢菲类，但与酸性物质一起反应时，可转化为产生荧光的酚类。用乙醇硫—酸或乙酸酐—二氯甲烷—硫酸处理后的DHCC进行荧光检测，获得标准曲线0—40μg/ml范围内呈线性关系，检出的最高灵敏度可达300ng/ml。该方法可用于分离后的DHCC及单一种DHCC代谢物的定性及定量分析。

objective to establish immunological methods specific for detecting antigens in different groups of monoclonal antibodies.methods indirect immnofluorescence assay was applied to identify specificity of the two groups of monoclonal antibodies prepared with crude antigen and recombinant antigen of aspergillus fumigatus,respectively.two different double monoclonal antibody sandwich elisa assays established with the two groups of antibodies were performed to detect antigents in the cell culture supermatants of 19 common species of aspergillus,penicillium marneffei,and 5 species of candidas.results the results of indirect immnofluorescence assay indicated that the monoclonal antibodies prepared with crude antigen of aspergillus fumigatus were specific for antigens in both clinical isolates and environmental isolates of aspergillus, whereas the other group of monoclonal antibodies was proved to be specific for aspergillus fumigatus of both clinical and environmental isolates.the elisa assay established with the crude antigen-specfic monoclonal antibodies could detect both of the clinical and environmental isolates of aspergllius, while the other assay could only detect aspergillus fumigatus of both clinical and environmental isolates.and no cross reaction with the cell culture of penialllium marneffei and candidas was observed with the two methods.conclusion the elisa assays can detect both of the clinical and environmental isolates of aspergillus,and differentiate aspergillus fumigatus from other species of aspergillus.

目的 用2组曲霉单克隆抗体建立特异性识别不同种类曲霉抗原的检测方法。方法采用天然烟曲霉抗原免疫，获得广谱针对曲霉抗原的单克隆抗体；采用重组烟曲霉抗原获得特异性针对烟曲霉抗原的单克隆抗体，用间接免疫荧光鉴定，并分别建立2种双抗体夹心elisa法，对19种常见的环境和临床分离曲霉株、马尔尼菲氏青霉菌及念珠菌培养液进行检测。结果间接免疫荧光显示，用天然烟曲霉抗原免疫获得的单克隆抗体(mabs-1)可广谱识别多种曲霉分离株，而重组烟曲霉抗原获得的单克降抗体(mabs-2)仅能特异性结合临床和环境分离的烟曲霉抗原。用mabs-1建立的双抗体夹心elisa法可检测19种常见曲霉株培养液；用特异性针对烟曲霉抗原单克降抗体(mabs-2)建立的双抗体夹心elisa法可特异性检测临床和环境分离株烟曲霉培养液；与其他曲霉株无交叉反应；2种双抗体夹心elisa法与马尔尼菲氏青霉菌及念珠菌培养液均无交叉反应。

Main works of the author:(1) Has developed a design of charge amplifier which using a kind of integrated chip replace a large number of leave components, though all the realized proposal of measurement circuit for the piezoelectric accelerometer;(2) Has made the circuit board;(3) Has proposed a programme-controlled scheme in the sensitivity throw-over of charge convert and gain throw-over of output amplifier;(4) Has done the experiment, give the experimental result;(5) Has done the error analysis based on the experimental result and the principle of the charge amplifier.

作者完成的主要工作包括：(1)通过对压电加速度传感器的测量电路的各种实现方案的深入调研，提出了一种用集成芯片取代大量分离元件的电荷放大器的实现方案；(2)制作了完整的电路板；(3)在电荷转换部分的灵敏电容的切换和输出放大级的增益切换电路的实现上提出了程控的方案；(4)进行了实验验证，给出了实验结果；(5)在实验结果的基础上，结合电荷放大器原理对系统作了误差分析。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。