分离点

Methods Marrow-derived mesenchymal stem cells were isolated and purified from mouse femoral bone and shinbones using differential adherent methods. Cells at the third passage were induced by 20% FBS in conditioned medium, conditioned medium alone, 20% FBS or 10% FBS alone respectively. Mouse aortic smooth muscle cells were cultured as the positive control. Levels of mRNA and protein expression of myoeardin and several smooth muscle cells marker genes were determined by immumofluorescenee, RT-PCR and Western blot before and 3, 7, 10, 14d after the induction. The presence of smooth muscle myofilaments was detected by using transmission electron microscope.

采用伞骨髓贴壁法分离骨髓问充质干细胞，CM联合20%FBS诱导骨髓间充质干细胞，同时设立持续10%FBS、单20%FBS及单CM诱导下的骨髓间充质十细胞对照组和SMC阳性对照组，分别于诱导前及诱导3、7、10和14 d时观察细胞形态的变化，并在相应的时间点用免疫荧光法、逆转录聚合酶链反应法、Western blot半定量分析法榆测myocardin以及SMC表面各种标志基因的表达变化，用透射电镜检测诱导后细胞内肌丝存在以此来证实诱导分化成功。

This study ac- quired an appropriate protein extraction method from ovules of Chinese pine and optimized the conditions for protein identification by mass spectrometry.

将电泳分离得到的蛋白点用肽质量指纹图谱的方法进行鉴定，得到了满意的结果，优化了蛋白质质谱鉴定的条件。

The sampling sites were divided into Suaeda salsa land, Spartina land and beach according to the vegetation. The microorganisms were isolated and counted with pour plate technique.

根据植被不同，将采样点划分为盐蒿带、米草带和光滩带，采用稀释平板法进行微生物分离计数。

The permanent wilting point is reached when the plant has wilted to a point where it will not recover, even if more water is added, because the cells have plasmolyzed.

当植物的萎蔫达到一定程度就不可以再复原，这一极限被称为永久萎蔫点。超过此极限，补充再多水分也不能被吸收，因为已经胞质浓缩已经到达质壁分离的程度。

As applications of the method, the linear wave making problems of Wigley hull and Series 60 hull are solved, with less collocation-points than that in traditional panel method, and the Kelvin wave-patterns calculated are in accord with the test data. Reasonable Kelvin wave-pattern and wave resistance of a high-speed round-bulge ship with transom stern are obtained by enforcing a condition, resembling Kutta-type condition, of smooth flow detachment at the transom stern. In addition to that, the linear solution of a catamaran is also presented.

本文运用该方法求解了Wigley船型与系列60船型的线性兴波问题，以较少的配置点计算出了与实验结果相近的Kelvin波系及合理的波形阻力；以在方尾部强加类似于库塔条件的光滑分离边界条件求解了高速圆舭型方尾船的线性兴波问题，得到了合理的三维波型及波形阻力；另外还求解了双体船的线性兴波问题。

And this paper also presents the klo-RANSAC technique,to extract rotational surface from the noise and the back ground data,which improves the performance and robustness greatly.

算法使用基于特征约束的klo-RANSAC（k-local-RANSAC）算法将特征点从自由曲面背景以及噪声中提取和分离，进一步提高了算法的收敛速度与健壮性。

On a grating type gear integrated error measuring equipm ent, this technique employs the three-point method error separation technique fo r separation of the systematic error of the measuring chain of equipment spindle system including measuring worm error, grating transducer error, bearing rota ry error etc.

在一台光栅式齿轮整体误差测量仪上，它用三点法误差分离技术能分离开仪器轴系测量链的系统误差(包括测量蜗杆误差、光栅传感器误差、轴承回转误差等)和被测齿轮的全谐波误差。

Association of two homologous chromosomes began to separate, but in many of the points referred to as cross They also linked together.

联会的两条同源染色体开始分离，但在许多称作交叉的点上它们还连在一起。

One hour later, sarre dosage of oleic acid was injected through one side femoral vein by same wayAll rats were killed 3h,6h after operation, respectively.

分别在静脉注药后3h、6h2个时间点处死动物，左心室完全取血，分别放入试管中，分离血清或血浆，待测ACE、AⅡ、ET-1。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。