分离性

OBJECTIVE: To study the isolation of postembryonic pluripotent stem cells from fetal bone marrow, proliferative culture in vitro, induction and differentiation; transplantation to the liver of SCID mice with hepatic failure, and detect therapy effects.

目的：根据胚胎后亚全能干细胞表达CD105的特性分离胎儿骨髓源性胚胎后亚全能干细胞，经体内外诱导使其分化为肝细胞样细胞并检测其功能。

Cognitive behavior of the grown-up offspring that the clozapine-exposed in pregnance lead to the the retardation of the weight of filial rats in earlier age.

体格生长：子代大鼠出生后第4天和第7天，对照组和用药组大鼠的体重差异有显著性(F=12.56，P＜0.01；F=7.51，P＜0.01)11，17天则没有差别，显示氯氮平孕期给药可导致子代大鼠早期的体重增长迟缓；孕期使用氯氮平对子代大鼠耳廓分离，出牙时间，开眼时间没有影响。

The results indicate that (1) After acclimation and induction, Phosphorus Accumulating Organism not only has the characteristics of denitrification, but also has the characteristics of takeup phosphate in aerobic;(2) Denitrifying phosphate accumulating bacteria using NO3- as electron acceptor indeed existed in the conventional enhanced biological phosphorus removal system;(3) The facultative anaerobe seperated during the process of acclimation are Corynebacterium, Pseudodiphtheria corynebacterium, Staphylococcus and Moraxella; all of which can be functioned as denitrifying phosphorus accumulating to various degree.

试验结果表明：(1)好氧聚磷菌经驯化诱导后不但具有反硝化产氮特性，而且具有好氧聚磷特性；(2)在传统的A2O 工艺中存在有以NO3-为电子受体的反硝化聚磷菌；(3)在驯化过程中分离到的兼性厌氧微生物有棒状杆菌属、假白喉棒杆菌、葡萄球菌属及莫拉氏菌属，且都有不同程度的反硝化聚磷功能。

Pulseless electrical activity (PEA, formerly termed electromechanical dissociation) may indicate cardiac tamponade, tension pneumothorax, and/or profound hypovolemia.

无脉搏心电活动（PEA，先前称为电机械分离）可提示急性心包填塞、张力性气胸和/或重度血容量不足。

Results Gallbladder puncturation and internal jejunal drainage through stent has less operation duration and faster recovery of gastrointestinal tract recovery(P.01),less side effects(P.05),yet the jaund...

结论对一般情况较差、肿瘤靠近肝门部且分离胆总管、肝总管或左、右肝管困难者或手术耐受差的恶性梗阻性黄疸患者，选择经胆囊床穿刺支架空肠内引流术。

TheMn complexes with internal quaternary ammonium and/or pyridinium salt, either formed in-situ or isolated, displayedsignificantly higher activities than analogous complexes and Jacobsens catalyst in theaforementioned reaction.

通过研究发现，含有分子内季铵盐和／或吡啶盐的手性吡咯烷Mn配合物，无论是原位生成的还是分离的，都比类似的配合物以及Jacobsen催化剂的活性明显高。

Methods Combined normal silica pressure column with L-aa-OCH3HCl as chiral pool, four racemic mixtures were synthesized and separated.

利用普通的硅胶加压柱联合L-氨基酸甲酯盐酸盐作为手性衍生化试剂将具有同一结构式的4个外消旋混合物进行了分离。

A detail comparison demonstrates that such preconditioned IbpB is distinguished from the non-preconditioned IbpB by a remarkable conformational transformation, including a significant increase in the flexibility of the N- and C-terminal regions, as well as enhanced dynamic subunit dissociation/reassociation.

我们的比较研究表明，这样的经过热休克预处理过的 IbpB蛋白与未经历过热休克条件的蛋白之间存在很大的构象上的差异，包括前者在N-和C-末端区域可曲性的明显增强，以及亚基动态分离/重结合的加强。

The DNA of Yak PrP expressed plasmid extracted from the recombinant expressed bacterium was identified by PCR amplification and two-ezyme digestion. The expressed product was obtained from the recombinant bacterium inducted under the optimized expressed condition (37℃, 1 mmol/L IPTG, 6 h), and it was determined by SDS-PAGE and Western blotting. The GST-BoPrP(23—242) fusion proteins were collected by two ways: the first is to purify and renature from the inclusion bodies; the second is to separate by SDS-PAGE.

将保存的含有牦牛PrP基因重组表达菌提取质粒DNA，进行PCR扩增和双酶切鉴定，并在优化诱导表达条件(37℃，1mmol/L IPTG，6h)下，获得的表达产物进行SDS-PAGE和Western blotting检测；将鉴定之后的重组表达菌诱导表达后，通过两种方法回收GST-BoPrP(23～242)融合蛋白：其一，是从包涵体中提取和复性GST-BoPrP(23～242)融合蛋白；其二，是通过SDS-PAGE直接分离并纯化GST-BoPrP(23～242)融合蛋白。

Using volatile acid and alkali to regulate the pH of pressed juice from bamboo shell,saltless Fraction Ⅰand Fraction Ⅱwere got separately.the residue of squeezed banboo shell was used as paper making material and medium for cultivation mushroom.

采用挥发性酸和碱调节鲜笋壳压榨液 pH值，分别获得无盐Fraction Ⅰ和fraction Ⅱ；其压榨后的蒲渣作为造纸原料和栽培食用菌的基质；对分离Fraction Ⅰ和Fraction Ⅱ后的汁液，加入Fe2 +并超滤浓缩，经硫酸铵盐析和丙酮分级后，进行CM 2 2纤维素离子交换柱层析，以 pH5 。2乙酸钠分级洗脱，获得RZ为 3 。0以上的高纯竹笋过氧氧化物酶。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。