分离性

Firstly,we isolated clinically and induced artificially multi-resistant Escherichia coli,detected antibacterials intake by different resistant level strains with CCCP and fluorescence spectro method and ascerained existing active efflux system in this strains; Secondly, construced internal standard of quantitive RT-PCR and detected AcrAB mRNA level of different resistant E.coli with quantitive RT-PCR; Thirdly, prepared AcrA antibody and detected AcrAB protein level of different resistant E.coli; eventually,designed and constructed Taqman probe of AcrAB and detected AcrAB level of different resistant E.coli with fluorescence quantitive PCR.

临床分离及人工诱导大肠杆菌多重耐药株，氰氯苯腙结合荧光分光法检测不同耐药水平菌株对抗菌药物的摄入，确证主动外排系统的存在；构建了定量RT-PCR的内标准DNA，通过定量RT-PCR检测动物源性大肠杆菌AcrAB mRNA的水平；制备AcrA抗体，采用Western-Blotting检测不同耐药株AcrAB蛋白的表达水平；设计和构建AcrA、AcrB的Taqman探针，检测不同耐药菌株AcrAB的水平。

Recently, a series of acridone alkoids, which showed the inhibiting active for the nucleoside transport and could be used to treat several kinds of cancers, was isolated from the root bark of Citrus genus.

另外，近几年由本属植物的根皮所分离得到的acridone alkaloids 则具有抑制nucleoside transport 及广效性抗癌作用。

Process of flotation of acture ore is the following: firstly, adding Na_2CO_3, ZnSO_4, CSU-1 to the pulp while grinding, and thus in this condition of low Eh, marmatite is inhibited; Secondly, adding CSU-1 to the pulp out of grinding machine, then amesonite and iron sulfur mineral can be flotated using diethyldithiocarbamate as collector; High quality lead and antimony concentrate can be got by adding CSU-1 and calcareousness; Lastly, marmatite is flotated by adding calcareousness, CuSO_4, xanthe, and D-250 to get zinc concentrate.

小型试验研究表明，首先在脱硫粗选作业只加入适量的Na_2CO_3、ZnSOO_4、和CSU-1至磨矿机，利用磨矿机的研磨和磨机中的低电位环境，使铁闪锌矿受到抑制，而最容易浮的脆硫锑铅矿和黄铁矿、磁黄铁矿等硫化矿物则依然具有可浮性；在磨机出来的矿浆中分别加入CSU-1进一步抑制易浮的铁闪锌矿，然后加入乙硫氮将脆硫锑铅矿和硫化铁矿快速浮净，加入石灰和CSU-1，浮选分离出高质量的铅锑精矿；接着添加石灰、CuSO_4、黄药及2~#油选别铁闪锌矿。

We first isolated CD4+CD25+ T cells isolated from nonautoimmune DBA2/NZW F1 mice and adoptively transferred to NZB/NZW F1 mice, which spontaneously develop lupus.

我们首先从正常老鼠(DBA2/NZW F1)分离纯化出CD4+CD25+ T细胞当作正常的调性T细胞，并且利用尾静脉注射移植到红斑性狼疮小鼠中。

MAIN OUTCOME MEASURES: Effect of glutamine,γ-amido butyric acid, and naloxone on the excitability of acutely isolated frontal cortex pyramidal neurons.

主要观察指标：谷氨酸和γ氨基丁酸及纳洛酮对急性分离的FCX锥体细胞兴奋性的影响。

The method is characteristic and unique, and can be used for quality control of amomum tsao-ko.

不同产地草果油的指纹图谱中各成分均得到了较好的分离，具有特征性和唯一性。因此本方法可作为草果油质量的控制方法。

Flower sex expression of monoecious lines (ATA-A and ATA-B) and andromonoecious lines and their offsprings (F1 and F2 and BC1) was investigated. Results showed that the flower expression character of that monoecious lines of melon was controlled by a pair of genes at one single locus,and the Monoecious character was controlled by the dominant gene.

通过对甜瓜单性花系ATA-A、ATA-B及他们的杂交后代F1、F2、BC1的花性型分离情况进行统计分析研究后认为，甜瓜单性花性状由一对基因控制的质量性状，单性花性状由显性基因所控制，其遗传符合孟德尔遗传规律。

Independent component analysis was used to separate the fMRI data of 8 patients with anisometropic amblyopia and 11 healthy controls, and a goodness-of-fit analysis was applied at the individual subject level to choose the resting-state visual network.

采用独立成分分析(independent component analysis， ICA)这一数据驱动方法对8名屈光参差性弱视患者和11名正常对照的静息数据进行分离，并采用拟合度值(goodness-of-fit scores)分析挑选出静息视觉网络，将结果进行组内分析和组间分析。

In the present study, differential display polymerase chain reaction was used to isolate three antennal -specific mRNA from the moth of cotton bollworm. Sequence analysis indicated that A4-1 clone was homologous to the glutathione S-transferase family of proteins.

利用差异显示PCR的方法分离得到了3条棉铃虫触角专一性的mRNA片段，在GenBank中进行同源搜索表明，克隆A4-1与昆虫谷胱甘肽-S-转移酶基因家族具有很高的同源性。

The article discusses the nature between the nous of science and technology and the nous of the value, which is inevitable in seperation and with the same unlimited approachment from the theory. This approachment is the same as the human and social development phase of Marxism, targeting at the full freedom and completed liberation ultimately.

本文论述了科技理性与价值理性的本质内涵，从理论上证明了二者分离之必然性和同一的无限趋近性，这种趋近和马克思关于人类社会的发展阶段的论述是一致的，其终极目标指向人的全面自由和彻底解放。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。