分离性

Methods: 1. Experimental laparoscopic liver resection: We selected pigs as experimental models aiming to approach human beings physiological and anatomical condition. After CO2 pneumoperitoneum was established, the ligaments around liver were dissociated. Livers were cut off by the methods such as clamp dissection, microwave tissue coagulator, ultrasound dissector, Ligsure dissection, hand-assisted, short abdominal incision laparoscopy-assisted, and Endo-GIA switcher. The operation time and bleeding volume were compared between different methods of laparoscopic liver resection. The advantages and limitations of different methods were summarized. 2. Clinical laparoscopic liver resection: 15 cases with tumors in segment V of anterior right lobe or segments II, III of left lateral lobe or segment IV of left internal lobe were selected in this study. The liver diseases included hepatocellular cancer, hepatic cavernous hemangioma and hepatic focal nodular hyperplasia. The ligaments around liver were dissociated after CO2 pneumoperitoneum established. Laparoscopic liver resection were carried out by some of the methods combined such as clamp dissection, microwave tissue coagulator, hand-assisted, short abdominal incision laparoscopy-assisted, ultrasound dissector, rotation and suction dissector.

1、实验性腹腔镜肝切除：以猪为实验动物，旨在接近人体的生理、解剖环境下进行实验，建立操作空间后游离肝脏周围韧带，采用钳夹分离切肝法、微波刀切肝法、超声刀切肝法、Ligsure切肝法、手辅助腹腔镜肝切除法、小切口腹腔镜辅助切肝法、Endo-GIA切肝法等方法实施腹腔镜下肝切除，比较不同切肝方法的手术时间、出血量，总结各种方法的优越性及其局限性。2、临床腹腔镜肝切除：选择肿瘤位于肝右前叶第Ⅴ段、肝左外叶第Ⅱ、Ⅲ段及左内叶第Ⅳ段的15例患者，病种包括原发性肝癌、肝海绵状血管瘤、肝脏局灶性增生，建立气腹后游离肝脏周围韧带，采用钳夹分离断肝、微波刀固化后断肝、手辅助腹腔镜肝切除、小切口腹腔镜辅助肝切除、超声刀断肝、旋吸断肝等多种方法分离切线肝组织，暴露管道结构，再采用高频电凝、钛夹夹闭、缝扎止血、止血纱布覆盖、氩气刀止血、生物胶止血、Endo-GIA处理等多种方法相结合处理肝断面，统计手术完成情况、手术时间、手术出血量、术后并发症及术后住院时间等，评价腹腔镜肝切除的可行性及安全性。

Ascites syndromes disease is a common and ubiquitous disease in the industrial culture with turbot, which often results in turbot's massive mortalities. In June 2006, an outbreak of ascites syndromes disease of the cultured turbot Scophthalmus maximus occurred in an indoor farm in Shandong Province of China Through the isolation, purification as well as cultivation of the bacteria in the diseased fish, and by tracing the bacteria in the well water, rearing pond water along with the feeding trash fish, it was found that the bacterium RF-1 which existed in the trash fish was similar to the bacterium FS-1 in the diseased turbot; artificial infection test demonstrated that the bacteria RF-1 and FS-1 had the same infection symptoms for healthy turbots.

腹水病是工厂化养殖大菱鲆的一种常见病和多发病，常造成鱼类大规模的死亡。2006年6月山东某大菱鲆Scophthalmus maximus养殖场发生大规模腹水病，通过对病鱼细菌的分离、纯化和培养，对养殖井水、养殖池水和所用饵料小杂鱼进行细菌追踪调查，将从该养殖场所用小杂鱼中分离得到的细菌RF-1与鱼体分离的细菌FS-1进行比较，并对其进行人工感染试验，结果证明这2株菌对健康大菱鲆都具有很强的致病性，而且感染症状完全一致。

Ascites syndromes disease is a common and ubiquitous disease in the industrial culture with turbot, which often results in trubot's massive mortalities. In June 2006, an outbreak of ascites syndromes disease of the cultured turbot Scophthalmus maximus occurred in an indoor farm in Shandong Province of China. Through the isolation, purification as well as cultivation of the bacteria in the diseased fish, and by tracing the bacteria in the well water, rearing pond water along with the feeding raw fish, it was found that the bacterium RF-1 which existed in the trash fish was similar to the bacterium FS-1 in the diseased turbot; artificial infection test demonstrated that the bacteria RF-1 and FS-1 had the same infection symptoms for healthy turbots.

腹水病是工厂化养殖大菱鲆的一种常见病和多发病，常造成鱼类大规模的死亡。2006年6月山东某大菱鲆 Scophthalmus maximus 养殖场发生大规模腹水病，通过对病鱼细菌分离、纯化和培养，对养殖井水、养殖池水和所用饵料小杂鱼进行细菌追踪调查，将从该养殖场所用小杂鱼中分离得到的细菌RF-1与鱼体分离的细菌FS-1进行比较，并对其进行人工感染试验，结果证明这2株菌对健康大菱鲆都具有很强的致病性，而且感染症状完全一致。

Five kinds of bacteria were isolated from disease Meretrix meretrix and culture water (two isolated from the M. meretrix, three from the culture water) in Lvsi harbor, Jiangsu province. The results of infection showed that strain WG1702 can cause disease with the mortality rate 100 percent and the same symptoms as original. It was cued that strain WG1702 was the pathogenic bacteria and caused the death in large scare, a preliminary research was done on the bacteria including morphological, physiological, biochemical characteristics, phylogenetic position and pathogenic. Strain WG1702 was a straight-rod gram negative bacterium, the physiological and biochemical indicators were lysine decarboxylase, ornithine decarboxylase, reduction of NO3- to NO2-, mannitose, oxidase and methylred reaction positive, arginine decarboxylase, arginine dihydrolase, salicin and voges-proskauer negative.

本文从江苏吕泗文蛤养殖区发病文蛤和养殖水体中分离到5株优势菌(病蛤中分离到3株优势菌，养殖水体中分离到2株优势菌)，人工感染实验结果显示，菌株WG1702能引起供试文蛤发病，死亡率为100%，且发病症状与原发病症状相同，提示该菌是引起文蛤大面积死亡的主要致病菌，对其形态、生理生化特征、分类地位及致病性进行了初步研究，菌株WG1702为革兰氏阴性菌，直杆状，生理生化指标为：赖氨酸脱羧酶、鸟氨酸脱羧酶、硝酸盐还原、甘露糖、氧化酶、甲基红阳性，精氨酸脱羧酶、精氨酸双水解酶、水杨素、V.P阴性。

Armillaria luteo-virens is a ectomycorrhizal fungus symbiotic with some species of Kobresia in Tibet Plateau, China. A strain was recently isolated from fruitbody tissue of the fungus. To determine whether the strain is the pure culture of the fungus, the internal transcribed spacer region and the first intergenic spacer region (IGS-1) of the nuclear ribosomal DNA of fruiting body and the isolated strain were sequenced and compared. Further, the 5.8S/ITS and IGS-1 sequences of the species were BLAST matched with GenBank database on homology and a phylogenetic tree was constructed based on 5.8S/ITS sequence and two phylogenetic trees were constructed separately based on two IGS-1 sequences.

首次从采自青藏高原、与高原牧草嵩草属Kobresia草本植物形成外生菌根的黄绿蜜环菌Armillaria luteo-virens子实体中分离获得一组织分离菌株，运用rDNA-ITS和rDNA-IGS-1测序技术对该组织分离菌株是否为黄绿蜜环菌的纯培养菌种进行分子鉴定，并基于黄绿蜜环菌的5.8S/ITS和IGS-1序列进行核酸序列数据库GenBank同源性检索比对、构建系统发育树。

Among them, strains of Bacillaceae occupied the predominance with 59.1% in various wheat organs. Oxytetracycline resistant strains of Bacillus licheniformis were isolated from wheat rhizosphere, root and stem indicating their high abilities of spreading. Opportunistic human pathogenic bacterial strains with oxytetracycline resistance such as Bacillus cereus and Stenotrophomonas sp. were also isolated from various wheat organs. No oxytetracycline resistant bacterium could be detected from wheat seeds.

土霉素抗性的Bacillus licheniformis在小麦根际土壤、根、茎等器官中都分离得到表明该抗性菌种在小麦内生系统中具有较高的传播性；具有土霉素抗性的人类机会致病菌Bacillus cereus和Stenotrophomonas属的细菌在小麦的根际土壤、根以及叶子中也均分离到，但是在小麦种子中没有分离到任何土霉素抗性的内生菌。

We, then, following the Holy Fathers, all with one consent, teach men to confess the one and same Son, our Lord Jesus Christ, the same perfect in Godhead and also perfect in manhood; truly God and truly man, of a reasonable soul and body; consubstantial with the Father according to the Godhead, and consubstantial to us according to the Manhood; in all things like unto us, without sin; begotten before all ages of the Father of the Father according to the Godhead, and in these latter days, for us and for our salvation, born of the Virgin Mary, the Mother of God, according to the Manhood; one and the same Christ, Son, Lord, Only-begotten, to be acknowledged in two natures, inconfusedly, unchangeably, indivisibly, inseparably; the distinction of natures by no means taken away by the union, but rather the property of each nature being preserved, and occuring in one Person and one Subsistance, not parted or divided into two persons, but one and the same Son, and only begotten, God the Word, the Lord Jesus Christ, as the prophets from the beginning concerning him, and the Lord Jesus Christ himself has taught us, and the Creed of the holy Fathers has handed down to us.

我们跟随圣教父，同心合意教人认识同一位子，我们的主耶稣基督，是神性完全、人性亦完全者；他真是上帝，也真是人，具有理性的灵魂，也具有身体；按神性说，他与父同体；按人性说，他与我们同体；在凡事上与我们一样，只是没有罪；按神性说，在万世之前，为父所生；按人性说，在晚进时日，为求拯救我们，由上帝之母，童女马利亚所生；是同一基督，是子，是主，是独生的，具有二性，不相混乱，不相交换，不能分开，不能离散；二性的区别不因联合而消失；各性的特点反得以保存，会合于一个位格，一个实质之内；而非分离成为两个位格，却是同一位子，独生的，道上帝，主耶稣基督；正如众先知论到他自始所宣讲的，主耶稣基督自己所教训我们的，诸圣教父的信经所传给我们的。

For confirmation of this ancient legend and found out the sexual improvement compounds the testosterone and NO in serum of mice were measured,the weight of sexual organs of mices was detected and the sex behaviour of mice was observed.The testosterone and NO concentration in serum of mice as indicator for screening some improving sexual compounds from Chinese chives was builted.1、The preliminary experiment of Chinese chives confirm sexual improvement functionThe sexual improvement function experiments of Chinese chives comparing with herba epimedii,herba cynomorii and radices morindae which were acknowledged as chinese herbals having sexual improvement function were carried out.The result was showed that the Chinese chives was confirmed having sexual improvement function,though its efficacy was less than herba epimedii,herba cynomorii and radices morindae.

为了证实古代医学认为韭菜的壮阳功能，揭示韭菜起温肾壮阳作用的化学物质基础，开发其功能性物质成分，本实验首先应用韭菜酒精初提液对小鼠睾酮、NO含量、性器官、性行为等指标的测定，验证韭菜是否具有改善性功能作用，然后以小鼠睾酮、NO含量等为指标对韭菜中的壮阳物质进行逐步分离和改善性功能作用筛选，以鉴定出几种改善性功能的物质。1、韭菜改善性功能的验证将韭菜与淫羊藿、琐阳、巴戟天三种有温肾补养功能的中草药进行比较，发现韭菜改善性功能作用不如淫羊藿、琐阳、巴戟天壮阳中草药的效果明显，但是韭菜还是具有显著的改善性功能作用的(P＜0.001)。

The replacement of toxic and volatile organic solvents as reaction media or an efficient catalyst with environmentally acceptable alternatives are an area of tremendous importance in modern organic synthesis. Basing the review about solid-phase synthesis with simple separation, the liquid phase organic synthesis supported by the solution polyethylene glycol and the chemical reactions in the medium of PEG, herein we are going to study the synthesis of 1-amidoalkyl-2 -naphthols in presence of the solution catalyst or the solid catalyst and the synthesis of thiourea in PEG-400 medium.

难溶性载体试剂参与下的固相合成使分离操作简单易行；可溶性聚乙二醇支载下的液相合成显示出液相反应的稳定高效性，同时兼顾大分子聚合物容易沉降分离的优点；无毒且稳定的小分子PEG作为反应介质，往往表现出醚和醇的双重性质和功能。

Some compounds possessing chiral center themselves may be racemates and appear racemic phenomenon in the extraction and separation from medicine herbs, structure modification and chemical synthesis.

但是，由于含有手性中心的中药有效成分本身可能是消旋体、或者在分离提取、结构改造及化学合成时会出现外消旋化现象，使新药研制与开发中面临对映体成分的分离制备、体内手性药物分析及对映体特异性药代动力学研究等重大难题。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。