分离性

Sensitivity comparison between the novel one-step RT-PCR and DAS-ELISA for PVX revealed that the former method was 20 times more sensitive than the later one. The novel one-step RT-PCR system was also successfully tested with field samples of turnip mosaic virus in 8 different crucifer vegetable crops.

为验证该设想，本研究建立了一步RT-PCR技术扩增体系，并利用该体系扩增和分析了国内几种蔬菜作物部分CMV分离物的2b基因序列同源性，选择特定分离物的全长2b基因，先利用PVX侵染性载体初步分析其正义和反义序列间的互作，再构建植物表达载体转化烟草和番茄，并研究转基因烟草抗CMV的表现，获得了抗CMV的转基因烟草植株。

In Chapter 1, the ubiquity of chiral phenomena and its important role in various disciplines, the methods for enantiomer separation, the principles of direct chromatographic separation of enantiomers and the status of chiral CGC are discussed and/or reviewed.

第一章论述或评述了手性特征的普遍性和在众多学科中的重要性、手性分离方法、色谱直接分离手性化合物对映体的基本理论、手性气相色谱研究现状。

To explore the influence of reticuloendotheliosis virus and subgroup J avian leucosis virus infection on chicken cellular immunity,the mitogenic response to ConA of blood and spleen lymphocytes tested and analyzed with 3H-thymidine(3H-TdR) incorporation assay at different intervals after infection or co-infection of single or both viruses.

自1974年分离出网状内皮增生症病毒[1]和1991年分离出禽白血病病毒J亚群[2]以来，在我国先后分离出不同毒株的REV和ALV-J，并开展了病原学、流行病学、致病性和免疫反应以及预防诊断等方面的研究，揭示出不同的致病性，并且确认它们都可造成感染鸡群免疫抑制，成为一些

The effect of temperature on the chiral separation of the molecular imprinting polymer was investigated and the thermodynamic parameters were calculated.

考察了温度对分子烙印固定相手性分离的影响，计算了手性分离过程中的热力学参数。

The present invention is process of separating and assembling metallic single wall carbon nanotube and semiconductive single wall carbon nanotube synchronously. The process includes the following steps: photoetching silica substrate to prepare Au electrode, cutting intervals in the electrode with focused ion beam, soaking the electrode in DMF solution of single wall carbon nanotube, and applying electric field of 2-8 V magnitude and 1-10 Hz frequency.

本发明的目的在于提供一种金属性和半导体性单壁碳纳米管的同步分离与组装方法，该金属性和半导体性单壁碳纳米管的同步分离与组装方法包括如下步骤：通过常规光刻在含二氧化硅的衬底上制备金电极的步骤；利用聚焦离子束在电极上刻出若干个间隙的步骤；将上述电极浸入到单壁碳纳米管的DMF溶液的步骤；施加大小为2～8V、频率为1～10Hz的电场的步骤。

Within this context, microseparation and in vitro culture of early zygotic embryos from polyembryony Citrus is very important because it makes possible the use of immature hybrid embryos and accelerates the sexual hybridization breeding in Citrus.

本研究初步建立了多胚性柑桔早期合子胚的显微分离、离体培养及合子苗的鉴定体系，成功显微分离了柑桔早期合子胚并通过离体培养再生植株，具体结果如下： 1、多胚性柑桔早期合子胚显微分离的最适时期为授粉后50—55d。

The results showed that Non-haemagglutinating activity was showed in the isolate. In agar diffusion test,there was a clear precipitin band formation to anti-IBDV specific serum. 28-day-old chicks inoculated with the isolate showed disease,and the isolate was recovered from the tissues of these chickens. The 1 041bp specific fragment were amplified by RT-PCR with sequence-specific primers based on IBDV VP3 gene. The VP3 gene shared 97.7%~98.2% nucleotide identities to very virulent IBDV,97.7%~95.2% nucleotide identities with classical strains from seqiemce analysis.

结果表明，该分离病毒无血凝性，在琼脂扩散试验中能与抗IBDV特异性血清出现1条清晰的白色沉淀线；人工感染28日龄雏鸡出现与临床一致的病变，并回收到病毒；应用针对IBDVVP3基因的特异性引物进行RT-PCR，能扩增到长度为1041bp的特异性目的片段；序列分析发现分离毒VP3基因与IBDV超强毒和经典毒株的核苷酸同源性分别为97.7%~98.2%和95.2%。

The effects of racemate initial concentration, volume ratio of surfactant to organic solvent, pH value in internal or external phase, pH gradient from external to internal phase, ligand metallicion and system temperature on the performances of chiral separation were discussed.

摘 要：研究在含有Cu2+和N-n-十二烷基-L-脯氨酸手性配体的乳状液膜体系中手性萃取分离氧氟沙星对映体的效果，以及外消旋体的初始浓度、表面活性剂与有机溶剂的体积配比、内外水相pH值、从外水相到内水相pH值梯度变化以及配位金属离子与体系温度对液膜手性分离性能的影响。

The results show that jamesonite and pyrrhotite have good floatability in the pH range of 2-10 and marmatite has good floatability in the acid and it is difficult to separate these minerals. In the pH range of 6-8, the separation of jamesonite and pyrrhotite, marmatite is possible when using mercaptoacetic acid and mercaptoethanol as depressants. In case of the presence of Cu2+ ion, however, it is difficult to separate the three minerals. Infrared spectrum demonstrates that there are a number of function groups such as —SH,—COOH,—OH in the molecular structure of depressant.

结果表明：用丁黄药作捕收剂，脆硫锑铅矿和磁黄铁矿在pH 2~10范围内都有良好的可浮性，铁闪锌矿在酸性条件下可浮性好，三者分离困难；在pH 6~8之间，采用巯基乙酸和巯基乙醇作抑制剂，脆硫锑铅矿与磁黄铁矿和铁闪锌矿的分离是可能的；但铜离子存在时， 3种矿物之间的选择性分离是难以实现的。

Considering the resemblance between paper chromatography and electrophoresis on distinguishing mixture of different monosaccharides or amino acids and eluting of interest component,we have a try to separate the components of some amylases which have been hydrolyzed by paper chromatography before polyacrylamide gel electrophoresis.

考虑到纸层析技术与电泳技术在分离物质(都可以对糖类混合物或者氨基酸混合物进行分离)和后期处理(都可以将含有目标成分的部分进行洗脱)电泳技术的相似处，本文在使用电泳分离出F_1-ATP合酶之前，先对水解后的多糖进行了尝试性的纸层析分离。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。