分离地

Distribution and isolation of cellulolytic myxobacteriaWe assayed distribution of and isolated cellulolytic myxobacteria of several environmental types of samples, i.e.

为了更深入地研究和开发溶纤维素粘细菌资源，本文在溶纤维素群粘细菌的分离纯化、生态分布、系统分子分类、降解纤维素机制进行了初步探索。

The soil and bark samples collected from Yunnan, Hebei and Shanxi provinces of China, and also from Vietnam, were used as the materials in this experiment. And dung pellets of rabbits or goats that had been autoclaved can be used to induce many kinds of fruiting bodies of the Myxobacteria in different colors, sizes, and shapes. We isolated and purified Myxobacteria strains with the improved method which was found by our laboratory.

本实验以采集自越南以及我国的云南、河北、陕西各地的土样和各种树皮、腐木作为实验材料，利用兔、羊粪球和滤纸作为基质诱导出多种颜色丰富、形态多样的粘细菌子实体，采用本实验室摸索出的较为有效的纯化粘细菌的方法，在实验器材上用24孔细胞培养板代替传统的平皿，有效地缩短了粘细菌的纯化周期，提高了分离纯化的效率。

The avenge recovery rates of icariin and naringin were 100.04% and 100.23%, respectively.

用HPLC法梯度洗脱能将淫羊藿苷和柚皮苷较好地分离检测，提高时效，减少误差。

Isopentanol, nitrochlorobenzene, nitrotoluene and diethylbenzene were well separated in the range of optimal carrier gas velocity of 13~17cm/s.

SE-30系列毛细管色谱柱的最佳载气线这范因为13~17cm/s，且在最佳线速度下很好地分离了异戊醇、硝基氯苯、硝基甲苯及二乙苯同分异构体体系。

To prove the capability of 042BS to nodulate both soybean and alfalfa, constitutively expressed green fluorescence protein gene (gfp carried by pMP2444) was introduced to 042BS, and the recombinant strain 042BSG was obtained. The reisolates from nodules of the soybean and alfalfa inoculated with 042BSG were observed using the confocal laser-scanning microscope, and the expressions of gfp were detected, respectively.

将其接种保定苜蓿和北引1号大豆，并重新分离出根瘤菌，在激光共聚焦荧光显微镜下检测，可以清楚地观察到标记基因的表达，从而确证了042BS能同时在苜蓿和大豆上结瘤。

Based on this idea, the author has chosen two typical loess sections of Luochuan, and Xifeng for this study, collected the successive loess columns; To probe the conditional experiments of BeO sample preparation and measurement, and improve the existing method; On the basis of the technology improvement, the author has finished 402 samples' BeO preparation and AMS measurement; Also at the same time, to separate quartz from samples at 1 cm interval for both Luochuan and Xifeng sections systematically, to carry out measurements of quartz grain-size, SUS, grey scale, carbonate content and bulk sample density; By using the grain size model, the author has set up the chronological framework for both Luochuan, and Xifeng sections and the time sequence of millennial scale abrupt climate events recorded in loess strata, and reconstructed the East Asian monsoon history over 130ka by using multi proxies; Finally, to discuss the possible mechanisms of palaeoclimatic changes tentatively.

详细地球科学的发展离不开高精度和高分辨率的研究。运用长寿命核素如〓Be作为环境过程的示踪，已成为不可替代的有效工具。本论文选择洛川、西峰两个典型的黄土剖面，连续采集柱状样；探索了BeO样品制备、测量的条件实验，改进了原有的方法，完成了402个BeO样品的制备和AMS测量；同时还系统地分离洛川、西峰剖面高分辨率（1cm）黄土、古土壤样品中的碎屑石英，测量了碎屑石英粒度，以及全样的磁化率、灰度、碳酸盐含量和容重等古气候代用指标；采用粒度年代模式建立了洛川剖面和西峰剖面的年代标尺，建立了黄土地层记录的千年尺度的气候突变事件的时间序列，多指标重建了最近130ka东亚季风变迁史；初步讨论了古气候变迁的可能机制。

Endophtic bacteria strains were isolated from healthy tobaccos from heavy disease plots in Yongzhou, Ningxiang and Liuyang. By Laboratory dual culture test, seventeen actinomycetes to Phytophthora parasitica var. nicotianae were screened.

同时在湖南永州、浏阳和宁乡等地发病重的田块采集健株共分离到内生细菌120株，经室内平皿对峙培养法测定，其中8株内生细菌菌株在室内有较强的拮抗作用。

These methods are useful to se parate the constituents of stellera chamaejasmel.

用此实验方法可有效地分离瑞香狼毒的化学成份。

NOTE 5—If confirmation of channels or incomplete seal areas in peelable packages is necessary, hand peel such suspected package completely separating the two material components and inspect the seal area of the transferred adhesive for the same incomplete seal attributes as the unopened package.

如果渠道或在peelable包裹的残缺不全的密封区的确认是必要的，递果皮完全地分离二个物质组分的这样被怀疑的包裹并且检查转移的胶粘剂的密封区残缺不全的封印属性和未打开的包裹一样。

In accordance with the invention, the hG-CSF protein can be prepared with high purity through rather simple process facilitating secretion of large amount of hG-CSF fusion protein into the periplasm, which does not require complicated processes such as solubilization and subsequent refolding required for isolation of the hG-CSF protein produced in cytoplasm as insoluble inclusion bodies by conventional techniques, thus, the hG-CSF protein can be widely used as an active ingredient in the development of supplementary agents for anticancer therapy.

根据本发明，通过促进大量hG－CSF融合蛋白分泌到周质中的相当简单的方法可以高纯度制备hG－CSF蛋白，所述方法不需要复杂的加工过程例如对于通过常规技术分离胞质中产生的作为不溶性包函体的hG－CSF所需的溶解和随后再折叠，因此，在开发用于抗癌治疗的补充药物的过程中可用hG－CSF蛋白广泛地作为活性成分。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。