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Joba knows his dad's history, how he was apart from his family, which lived on the Winnebago Indian Reservation, 107 miles north of Lincoln.

Joba知道父亲的过去,他如何与他的亲生家庭分离,就在距离林肯市北方107里远的印地安保留区。

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果:1、哇巴因作用于晶状体钠泵后,光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙,电镜下全层细胞空泡化、线粒体肿胀出现髓样结构,RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后,光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离,电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失,RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后,光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整,电镜下细胞器丰富、细胞功能旺盛,RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后,光镜下晶状体质膜完整、细胞排列紧密规整,电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝,RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后,光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化,电镜下囊侧膜内有高电子密度带、细胞核仁丰富,RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后,光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏,电镜下质膜变性、线粒体肿胀,RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

Six strains of Phytophthora parasitica var. nicotianae were isolated from disease tobaccos in Yongzhou and Ningxiang from heavy disease plots;. Pathogenicity was tested to six strains, the results shows the strain of phy3 have strong pathogenicity. We also studied the effect of several solutions for producing zoosporangium in Artificial culture condition, the result shows the hyphea culture with 0.1% KNO3 produced the most quantity of zoosporangiums.

2001~2003年,从湖南永州和宁乡等地发病重的田块采集病株共分离到烟草黑胫病菌(Phytophthora parasitica var.nicotianae)菌株6株,经离体叶片致病力筛选,烟草黑胫病菌株phy3为强致病力菌株,病情指数达到89%;并在实验室条件下,研究了几种溶液诱导孢子囊的产生效果,结果证明经0.1%KNO,处理产生孢子囊最多。

A new painting way evading Chinese traditional ones may start here, the trial. Through these conceptual works, we can find that Chinese artists gradually step further and further from the fondness for portrait and plot, get rid of the embarrassed state of traditional paintings and separate from the acuminous and logic visional experience.

这样的尝试可能会为中国逃避传统的绘画方式拉开序幕,那么,透过这些概念化的作品,我们可以窥见中国艺术家在最近的几十年中,已逐渐地游离开传统绘画对物象和情节的迷恋,并摆脱了常规绘画的尴尬处境,从中分离出敏锐的、逻辑的视觉体验。

But,because this right blurs the function of the prosecutor and the judge,violates the principle of separating prosecute and adjudicature,ruins the neutrality of judges,harms the equality of judgments,makes mass in beth procedure theories and judicial practice.Therefore,it is desirable to eliminate the judge's right to investigate outside the court,and accordingly,measures should be taken to meet this requirement.

但是,由于它混淆了侦查与审判的职能,违背了诉审分离的原则,破坏了法官的中立形象,损害了审判的公正,给诉讼理论和司法实践都造成了混乱,因此,建议取消法官的庭外调查权,相应地完善有关的配套措施。

Based on this, a rapid,simple and accurate method for the determination of hexavalent chromium in alkalescent soil has been developed.

结果表明,用0.4mol/LKCl为提取剂,离心分离提取液,可简单、快速、准确地测定土壤中Cr6+,所建立的方法适用于北方偏碱性土壤。

Transgenic plants expressing GNA showed significant insecticidal activity towards \\omoptera insects such as aphids and brown planthopper in bioassay and feeding tests.In this paper a lectin gene was firstly isolated from leaves of Zephyranthes grandiflora, belonging to Amaryllidaceae, by the use of rapid amplification of cDNA ends technique. Characterization, functional prediction and construction of plant and prokaryotic expression vector of this novel gene were studied.

本实验采用cDNA末端快速扩增(Rapid amplification of eDNA ends,RACE)技术,首次从中国特有的野生植物韭莲(又名风雨花,Zephyranthes grandiflora)的叶片中分离克隆出了一个新的凝集素基因,对基因进行了序列分析和功能预测,并成功地构建了韭莲凝集素基因的植物表达载体和原核表达载体。

The difference is that the collection, separation, and return are all performed inside a machine which is connected to the donor through a needle placed in the arm, typically the antecubital vein.

不同点在于收集、分离和返回过程都在一个机器内部操作,机器通过在胳膊上放置的针与捐献者相连,典型地针头的放置位置在肘前静脉。

RESULTS: Myocardial cells of the adult rats were preserved with ultrahypothermia liquid nitrogen storage and a sufficient number of bacilliform myocardial cells which met the quality standard were separated.

结果:采用成年大鼠心室肌细胞超低温液氮保存分离法,成功地分离出足够数量符合质量的杆状心肌细胞。

From the ester n, the intermediate Ⅲwas produced by the Sonogashira coupling reaction with terminal alkynes under the co-catalysts of Pd2 and Cul. These final products of alkynyl benzamide derivatives IV were obtained by the cleavage of support with primary or second amines.

液相合成兼容了固相合成可快速简便地对产物进行分离纯化以及溶液相合成可在均相条件下进行反应和用常规手段对反应中间体进行分析检测的优点,用聚乙二醇4000作为可溶性聚合物支持体,通过醚键将[1,3,5]氯三嗪连接在PEG上制备成PEG支持的新型的可溶性聚合物试剂I。

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Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车,和一双合并为一移交&整个lotta爱&。

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