分离

Methods: MSCs were isolated from human adult bone marrow through density gradient with Ficoll paque (1.077g/ml) and then amplified .

采用Ficoll淋巴细胞分离液(1.077g／ml)密度梯度离心法从人的骨髓中分离出骨髓间充质干细胞进行体外扩增。

Methods Rat bone mesenchymal stem cells were isolated from adult rats bone marrow by Ficoll -Paque density gradients, cultured and amplified in vitro, and then identified by flow cytometry ; Middle cerebral artery occlusion model of regional cerebral ischemia in rats were made by thread embolizing.

方法Fieoll-Paque分离液梯度离心分离大鼠骨髓间质干细胞（rat bone mesenchymal stem cells，rMSCs），经体外培养扩增并流式细胞术鉴定；线拴法制作大鼠大脑中动脉闭塞脑局灶缺血模型，Hoechst33342标记细胞，分别通过静脉移植及立体定向局部移植到大鼠缺血侧纹状体。

A normal-phase column was used to separate nonparaffinic and paraffinic materials without resolving the latter into individual components.

利用正相色谱柱对石蜡和非石蜡组分进行了分离，而无需对石蜡组分进行逐一分离。

The combination of Domain Decomposition Method and Monte Carlo method makes it possible to calculate parallelly in time and space zone.

通过引入辐射传递系数，将该系数的计算与能量方程的求解分离，从而分离了计算的难点，使得在时间域上能把整个问题分解为若干个子问题并行处理。

To control tobacco black shank disease rationally, reduce resistance, 34 strains of Phytophthora parasitica var. nicotianae were isolated from 6 region of Yunnan province and all isolates were determined their resistance to metalaxyl. The results indicated that of all tested isolates, there were 12 high resistance, 5 middle resistance and 17 low resistance isolates respectively.

为合理防治烟草黑胫病，减少抗药性产生，从云南省6个地州分离烟草黑胫病菌菌株34个，就分离菌株对甲霜灵的抗药性进行了测定，结果表明，供试34个菌株中中高抗菌株12个，中抗菌株比例较高5个，低抗菌株17个。

In addition, Mycoplasma bovis, E. coli, Salmonella, Streptococcus, Staphylococcus and Aspergillus fumigatus was successfully isolated from the lungs and other tissues, however, no Pasteurella was isolated.

从肺等组织中分离到牛支原体以及大肠杆菌、沙门氏菌、链球菌、葡萄球菌和烟曲霉，没有分离到巴氏杆菌。

Compared studies of routine DIF and DIF of 1mol/L Nacl-separated patients skin and IIF using 1mol/L NaCl-separated normal human skin as substrate were clone in 19 patients with bullous pemphigoid and 5 with epidermolysis bullosa acquisita.

对19例大疱性类天疱疮和5例获得性大疱性表皮松解症病人进行了常规DIF、1mol／LNaCl分离表皮DIF和1mol／LNaCl分离正常人皮肤IIF的对比研究。

Results Nine compounds from supercritical CO2 extraction were identified and the main constituents were pentadecane, ethyl pmethoxycinnamate and ethyl cinnamate. Twentyeight compounds from water distillation were identified and the main constituents were δ3carene, ethyl pmethoxycinnamate and ethyl cinnamate.

结果 沙姜超临界CO2萃取物中分离鉴定出9种化合物，主要成分为十五烷、肉桂酸乙酯、对甲氧基肉桂酸乙酯等；水蒸气蒸馏法中分离鉴定出28种化合物，主要成分为δ3蒈烯、肉桂酸乙酯和对甲氧基肉桂酸乙酯等。

Results Nine compounds from supercritical CO2 extraction were identified and the main constituents were pentadecane, ethyl p-methoxycinnamate and ethyl cinnamate. Twenty-eight compounds from water distillation were identified and the main constituents were δ-3-carene, ethyl p-methoxycinnamate and ethyl cinnamate.

结果 沙姜超临界CO2萃取物中分离鉴定出9种化合物，主要成分为十五烷、肉桂酸乙酯、对甲氧基肉桂酸乙酯等；水蒸气蒸馏法中分离鉴定出28种化合物，主要成分为δ-3-蒈烯、肉桂酸乙酯和对甲氧基肉桂酸乙酯等。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。